| 蒋文军,于晓倩,史晨毅,田 姗,刘 瑞,高子军.苍耳子提取物抗类过敏作用及活性成分筛选的研究[J].现代生物医学进展英文版,2022,(17):3238-3243. |
| 苍耳子提取物抗类过敏作用及活性成分筛选的研究 |
| Effect on the Anti-anaphylactoid Reactions of Fructus Xanthii Extract and Screen Its Active Ingredients |
| Received:January 26, 2022 Revised:February 23, 2022 |
| DOI：10.13241/j.cnki.pmb.2022.17.007 |
| 中文关键词: 苍耳子 类过敏反应 肥大细胞 MrgX2受体 |
| 英文关键词: Fructus Xanthii Anaphylactoid reactions Mast cells MrgX2 recepter |
| 基金项目:国家自然科学基金项目（81903572）；中国博士后科学基金项目（2019M663755）；陕西省自然科学基础研究计划项目（2019JQ-979） |
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| 中文摘要: |
| 摘要 目的：探讨苍耳子提取物抗类过敏作用及筛选出其中的活性成分。方法：采用测量小鼠脚掌肿胀和组织液渗出，检测小鼠血清中组胺、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、单核细胞趋化因子-1（monocyte chemotactic protein-1，MCP-1）和白细胞介素-8（interleukin-8，IL-8）浓度，检测肥大细胞脱颗粒的方法，观察苍耳子提取物拮抗C48/80诱发的类过敏反应的作用。建立高表达MrgX2/CMC筛选模型，从苍耳子的10种成分中筛选可作用于MrgX2受体的抗类过敏活性成分。结果：苍耳子提取物可减轻C48/80导致的小鼠脚掌肿胀和组织液渗出，降低小鼠血清中组胺、TNF-α、MCP-1和IL-8的含量，抑制肥大细胞脱颗粒（P<0.05）。10种苍耳子成分中筛选出6种有保留成分，其中槲皮素和大黄素可显著抑制肥大细胞释放组胺（P<0.05）。结论：苍耳子提取物可抑制肥大细胞脱颗粒和致敏介质的释放，其成分中槲皮素和大黄素具有潜在的抗类过敏作用。 |
| 英文摘要: |
| ABSTRACT Objective: The aim of this article is to investigate the anti-anaphylactoid effect of Fructus Xanthii extract and screen its active ingredients. Methods: The anti-effect of Fructus Xanthii extract on c48/80 induced anaphylactoid was investigated by measuring paw thickness increasing and tissue fluid exudation in mouse model, detecting concentration of histamine, tumor necrosis factor-α (TNF-α), monocyte chemotactic protein-1 (MCP-1) and interleukin-8 (IL-8) in mouse serum, detecting mast cells degranulation in LAD2 cells. A MrgX2 high-expression HEK293 cells membrane chromatography (MrgX2/CMC) screening model was established, and the anti-anaphylactoid active ingredients that targeted on MrgX2 receptors were screened from 10 ingredients of Fructus Xanthii. Results: Fructus Xanthii extract reduced mouse paw thickness increasing and tissue fluid exudation, reduced the release of histamine, TNF-α, MCP-1 and IL-8 in mouse serum, and inhibited the degranulation of LAD2 cells. There were 6 ingredients retained in the MrgX2/CMC model from the 10 components of Xanthii fructus, among which Quercetin and emodin could significantly inhibit the release of histamine in LAD2 cells. Conclusion: Fructus Xanthii extract can inhibit the degranulation of mast cells and the release of allergic matter induced by C48/80, and quercetin and emodin from its ingredients have potential anti-anaphylactoid effects. |
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