| 王 敏,卢怀海,庞旭阳,朱海生,王旭东.川陈皮素对脑梗死缺血再灌注大鼠的保护作用及其机制研究[J].现代生物医学进展英文版,2022,(14):2642-2647. |
| 川陈皮素对脑梗死缺血再灌注大鼠的保护作用及其机制研究 |
| Protective Effect of Tangerine on Rats with Cerebral Infarction and Its Mechanism |
| Received:January 23, 2022 Revised:February 18, 2022 |
| DOI：10.13241/j.cnki.pmb.2022.14.008 |
| 中文关键词: 脑梗死 大鼠 缺血再灌注 川陈皮素 炎症细胞 凋亡基因 |
| 英文关键词: Cerebral infarction Rat Ischemia reperfusion Tangerine Inflammatory cytokines Apoptosis gene |
| 基金项目:河北省2018年度医学科学研究重点计划项目（20181685） |
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| 中文摘要: |
| 摘要 目的：研究川陈皮素对脑梗死缺血再灌注大鼠的保护作用及其机制。方法：将90只雄性Wistar大鼠随机分为空白对照组、假手术组、模型组及低剂量组、中剂量组及高剂量组，每组15只。空白对照组不予以任何处理，模型组、低剂量组、中剂量组及高剂量组其余各组大鼠均采用Long线栓法构建脑梗死缺血再灌注模型，假手术组除了不插入拴线，其他操作和模型组相同。造模后，空白对照组、假手术组及模型组分别予以10 mL/（kg?d）的生理盐水灌胃，低剂量组、中剂量组、高剂量组分别予以10 mL/（kg?d）、15 mL/（kg?d）、20 mL/（kg?d）川陈皮素灌胃。检测各组大鼠梗死面积以及神经细胞凋亡率，缺氧诱导因子-1α（HIF-1α）、血管内皮生长因子（VEGF）水平，炎症细胞因子水平，B淋巴细胞瘤-2（Bcl-2），Bcl-2相关X蛋白（Bax），半胱氨酸天冬氨酸蛋白酶-3（caspase-3）表达情况。结果：模型组、低剂量组、中剂量组、高剂量组梗死面积、神经细胞凋亡率以及HIF-1α、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-β）、Bax mRNA、caspase-3 mRNA水平均高于空白对照组及假手术组；且低剂量组、中剂量组、高剂量组较模型组低；降低呈剂量依赖性（均P＜0.05）。模型组、低剂量组、中剂量组、高剂量组VEGF、Bcl-2 mRNA水平均低于空白对照组及假手术组；且低剂量组、中剂量组、高剂量组较模型组高；降低呈剂量依赖性（均P＜0.05）。假手术组梗死面积以及神经细胞凋亡率均高于空白对照组（均P＜0.05）。结论：川陈皮素对脑梗死缺血再灌注大鼠的保护作用明显，其主要机制可能与调控HIF-1α、VEGF、炎症细胞因子以及凋亡相关基因表达有关。 |
| 英文摘要: |
| ABSTRACT Objective: To study the protective effect of tangerine on cerebral infarction ischemia reperfusion in rats and its mechanism. Methods: 90 male Wistar rats were randomly divided into blank control group, sham operation group, model group, low-dose group, middle-dose group and high-dose group according to the random number table method, with 15 rats in each group. No treatment was given to the blank control group. The model group, the low-dose group, the middle-dose group and the high-dose group all constructed the cerebral infarction ischemia-reperfusion model by using the Long line embolization method. The sham operation group performed the same operation as the model group except that the tetter was not inserted. After modeling, 10 mL/ (kg?d) of normal saline was given to the blank control group, the sham operation group and the model group, and 10 mL/ (kg?d), 15 mL/ (kg?d), and 20 mL/ (kg?d) of tangerine were given to the low-dose group, the middle-dose group and the high-dose group, respectively. The infarct area, apoptosis rate of nerve cells, hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), inflammatory cytokines levels, B-cell CLL/lymphoma 2(Bcl-2), Bcl-2 Assaciated X protein(Bax) and Cystein-asparate protease 3(caspase-3) expression were compared in each group. Results: The infarct area, apoptosis rate of nerve cells and mRNA levels of HIF-1α, Tumor necrosis factor-α(TNF-α), Interleukin-1β(IL-1β), Bax and caspase-3 in model group, low-dose group, medium-dose group and high-dose group were all higher than those in blank control group and sham operation group. Low dose group, medium dose group and high dose group were lower than model group, the decrease was dose-dependent (all P < 0.05). The mRNA levels of VEGF and Bcl-2 in model group, low-dose group, medium-dose group and high-dose group were lower than those in blank control group and sham operation group. Low dose group, medium dose group and high dose group were higher than model group, the decrease was dose-dependent (all P<0.05). The infarct area and nerve cell apoptosis rate in sham group were higher than those in blank control group (all P<0.05). Conclusion: Tangerine has a significant protective effect on cerebral infarction ischemia reperfusion rats, and its main mechanism may be related to the regulation of the expression of HIF-1α, VEGF, inflammatory cytokines and apoptosis-related genes. |
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