| 左斌生,刘媛瑞,徐建华,罗宇虹,张战锋.HPVm16E7刺激树突状细胞SOCS1基因沉默后对宫颈癌小鼠模型免疫效应的影响[J].现代生物医学进展英文版,2022,(11):2013-2017. |
| HPVm16E7刺激树突状细胞SOCS1基因沉默后对宫颈癌小鼠模型免疫效应的影响 |
| Effect of HPVm16E7 Stimulated Dendritic Cell SOCS1 Gene Silencing on Immune Effect of Cervical Cancer Mouse Model |
| Received:December 08, 2021 Revised:December 30, 2021 |
| DOI：10.13241/j.cnki.pmb.2022.11.003 |
| 中文关键词: 宫颈癌 树突状细胞 SOCS1 免疫治疗 |
| 英文关键词: Cervical cancer Dendritic cells SOCS1 Immunotherapy |
| 基金项目:广东省中医药局科研项目（20222053）；广东省教育厅基础研究重大项目(2017KZDXM019)；创新强院青年科研人才培优项目(2015QN08) |
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| 中文摘要: |
| 摘要 目的：在宫颈癌细胞TC-1的荷瘤小鼠模型中探讨树突状细胞（DCs）的细胞因子信号抑制物1（SOCS1）基因沉默后对宫颈癌细胞免疫效应的影响。方法：构建宫颈癌细胞系TC-1的荷瘤小鼠模型，将含有SOCS1沉默基因的慢病毒载体感染DCs细胞，对荷瘤小鼠进行细胞免疫后监测小鼠体内肿瘤生长、小鼠存活率，并检测小鼠脾细胞对TC-1细胞的体外裂解率以及γ干扰素（IFN-γ）、白细胞介素-12（IL-12）表达等指标。结果：DCs SOCS1基因沉默后可延长小鼠存活期，增强DCs对小鼠体内肿瘤生长的抑制作用，增强小鼠脾细胞对TC-1细胞的体外裂解率(P<0.05)，增加小鼠血清IL-12因子表达(P<0.05)和小鼠脾细胞IFN-γ表达(P<0.05)，但对小鼠脾内效应细胞的数量没有影响(P>0.05)。结论：小鼠体内实验初步证实，DCs SOCS1基因沉默后可一定程度上增强小鼠体内效应细胞对肿瘤细胞的杀伤效果。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of suppressor of cytokine signaling (SOCS1) gene silencing of dendritic cells (DCs) on the immune effect of cervical cancer cells in the tumor bearing mouse model of cervical cancer cell TC-1. Methods: The tumor bearing mouse model of cervical cancer cell line TC-1 was constructed. The lentivirus vector containing SOCS1 silencing gene was infected with DCS cells. After cellular immunization of tumor bearing mouse, the tumor growth and survival rate of mouse were monitored, the cleavage rate of spleen cells to TC-1 cells in vitro and the expression of γinterferon (IFN-γ) and interleukin-12 (IL-12) were detected. Results: DCs SOCS1 gene silencing can prolonged the survival of mouse, enhanced the inhibitory effect of DCs on tumor growth in vivo, enhanced the lysis rate of mouse spleen cells to TC-1 cells in vitro(P<0.05), increased the expression of IL-12 factor in serum of mouse(P<0.05) and IFN-γ expression of mouse spleen cells(P<0.05). However, the number of effector cells in spleen of mouse was not affected(P>0.05). Conclusion: In vivo experiments in mouse show that DCs SOCS1 gene silencing could enhance the killing effect of effector cells on tumor cells in mouse to a certain extent. |
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