| 林文毅,欧阳慧,周晓宁,张艳玲,翁美娟.叶酸抑制宫颈癌进展过程中miR-642a-5p的表达及其对肿瘤细胞的抑制作用研究[J].现代生物医学进展英文版,2022,(7):1211-1215. |
| 叶酸抑制宫颈癌进展过程中miR-642a-5p的表达及其对肿瘤细胞的抑制作用研究 |
| Folic Acid Inhibits the Expression of miR-642a-5p during the Progression of Cervical Cancer and Its Inhibitory Effect on Tumor Cells |
| Received:October 06, 2021 Revised:October 29, 2021 |
| DOI：10.13241/j.cnki.pmb.2022.07.003 |
| 中文关键词: 叶酸 宫颈癌 miR-642a-5p 细胞增殖 细胞迁移 细胞侵袭 |
| 英文关键词: Folic acid Cervical cancer miR-642a-5p Cell proliferation Cell migration Cell invasion |
| 基金项目:广东省自然科学基金项目（2015A030313606） |
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| 中文摘要: |
| 摘要 目的：探讨叶酸抑制宫颈癌进展中过程中miR-642a-5p的表达及其对肿瘤细胞的抑制作用。方法：选择宫颈癌细胞株SiHa进行传代培养，采用随机法分为4组：低剂量组、中剂量组、高剂量组分别加入1 μg/mL、10 μg/mL、100 μg/mL的叶酸，而空白对照组未加入叶酸处理。利用实时定量聚合酶链式反应（RT-qPCR）检测miR-642a-5p的表达；采用细胞增殖毒性试验（CCK-8法）、细胞迁移实验（划痕实验）和细胞侵袭实验（Transwell法）分别测量宫颈癌SiHa细胞的增殖活性、迁移和侵袭能力。结果：低、中、高剂量组中叶酸显著抑制了宫颈癌SiHa细胞中miR-642a-5p的表达（P<0.05），而且随着叶酸浓度升高，其对miR-642a-5p的抑制作用逐渐增强。此外，低、中、高剂量组中叶酸对宫颈癌SiHa细胞的增殖、迁移以及侵袭具有显著抑制作用（P<0.05），而且叶酸浓度越高，其抑制作用越强。结论：叶酸可以抑制宫颈癌进展过程中miR-642a-5p的表达，而且对宫颈癌SiHa细胞的增殖、迁移和侵袭具有一定的抑制作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the inhibitory effect of folic acid on the expression of miR-642a-5p and tumor cells in the progression of cervical cancer. Methods: The cervical cancer cell line SiHa was selected for subculture and randomly divided into four groups: low dose group, medium dose group and high dose group were added with 1 μg/mL, 10 μg/mL and 100 μg/mL folic acid respectively, while the blank control group was not treated with folic acid. The expression of miR-642a-5p was detected by real-time quantitative polymerase chain reaction (RT-qPCR); The proliferation activity, migration and invasion ability of cervical cancer SiHa cells were measured by cell proliferation toxicity test (CCK-8 method), cell migration test (scratch test) and cell invasion test (transwell method). Results: Folic acid in the low, medium and high dose groups significantly inhibited the expression of miR-642a-5p in cervical cancer SiHa cells (P<0.05), and as the concentration of folic acid increased, its inhibitory effect on miR-642a-5p gradually increased. In addition, folic acid in the low, medium and high dose groups had a significant inhibitory effect on the proliferation, migration and invasion of cervical cancer SiHa cells(P<0.05), and the higher the concentration of folic acid, the stronger its inhibitory effect. Conclusion: Folic acid can inhibit the expression of miR-642a-5p during the progression of cervical cancer, and it has a certain inhibitory effect on the proliferation, migration and invasion of cervical cancer SiHa cells. |
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