| 何 杨,肖 帅,李 逦,曾婷艳,唐 俊.富血小板纤维蛋白对人牙周膜细胞成骨能力、炎症因子表达和 Wnt/β-catenin信号通路的影响[J].现代生物医学进展英文版,2022,(6):1180-1185. |
| 富血小板纤维蛋白对人牙周膜细胞成骨能力、炎症因子表达和 Wnt/β-catenin信号通路的影响 |
| Effects of Platelet-rich Fibrin on Osteogenic Ability, Expression of Inflammatory Factors and Wnt/β-Catenin Signaling Pathway in Human Periodontal Ligament Cells |
| Received:August 23, 2021 Revised:September 18, 2021 |
| DOI：10.13241/j.cnki.pmb.2022.06.038 |
| 中文关键词: 富血小板纤维蛋白 人牙周膜细胞 成骨能力 炎症因子 Wnt/β-catenin信号通路 |
| 英文关键词: Platelet-rich fibrin Human periodontal ligament cells Osteogenic ability Inflammatory factors Wnt/β-catenin signaling pathway |
| 基金项目:湖南省卫生健康委员会科研计划课题项目（B2017198） |
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| 中文摘要: |
| 摘要 目的：探究富血小板纤维蛋白（PRF）对人牙周膜细胞（hPDLCs）成骨能力、炎症因子表达和 Wnt/β-连环蛋白（Wnt/β-catenin）信号通路的影响。方法：通过刮取法、组织块法获取 2019年 1月至 2020年 1月期间我院口腔科收治的 10例正畸患者拔除健康阻生牙或正畸牙的牙周组织作为研究样本。将 hPDLCs细胞分为干预 1组、干预 2组、对照组、肿瘤细胞坏死因子 -α（TNF-α）组，各组均采用含有 10% FBSDMEM培养基培养，TNF-α 组加用 10 ng/mL TNF-α 诱导液，干预 1组加用 50% PRF培养，干预 2组加用 10 ng/mL TNF-α 诱导液和 50% PRF。对比各组的细胞增殖、碱性磷酸酶（ALP）活性、炎症因子、Wnt/β-catenin信号通路关键因子的表达差异。结果：（1）相比于其他 3组，干预 1组的吸光度值（OD）明显升高，相比于 TNF-α 组，干预 2组、对照组的 OD值均明显升高（P<0.05）；（2）和其他 3组相比，干预 1组的 ALP活性明显升高，相比于 TNF-α 组，干预 2组、对照组的 ALP活性均明显升高（P<0.05）；（3）和其他 3组相比，干预 1组的骨形态发生蛋白 2（BMP2）mRNA、runt相关转录因子 2（Runx2）mRNA、BMP2及 Runx2蛋白表达水平均明显升高，相比于 TNF-α 组，干预 2组、对照组的 BMP2 mRNA、Runx2 mRNA、BMP2及 Runx2蛋白表达水平均明显升高（P<0.05）；（4）相比于干预 1组、对照组，干预 2组、TNF-α 组的 TNF-α mRNA表达水平明显升高（P<0.05）；（5）和其他 3组相比，干预 1组的细胞周期蛋白 D1（CyclinD1）mRNA、β-catenin mRNA表达水平明显升高，相比于 TNF-α 组，干预 2组、对照组的 CyclinD1 mRNA、β-catenin mRNA表达水平均明显升高（P<0.05）。结论：PRF可以促进 hPDLCs细胞增殖和成骨，并减少炎症因子表达，提升 ALP活性，其可能是通过激活 Wnt/β-catenin信号通路促进 CyclinD1、β-catenin mRNA表达加快hPDLCs细胞成骨。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of platelette-rich fibrin (PRF) on osteogenic ability, expression of inflammatory factors and Wnt/β-catenin (Wnt/β-catenin) signaling pathway in human periodontal ligament cells (hPDLCs). Methods: Periodontal tissues of 10 orthodontic patients who were admitted to the Department of Stomatology from January 2019 to January 2020 were extracted from healthy impacted teeth or orthodontic teeth as research samples by curettage and tissue block methods. The hPDLCs cells were divided into intervention group 1, intervention group 2, control group and tumor necrosis factor-α (TNF-α) group. All groups were cultured with 10%FBSDMEM medium. TNF-α group was added with 10 ng/mL TNF-α induction medium, and intervention group 1 was added with 50% PRF culture. Intervention group 2 was supplemented with 10 ng/mL TNF-α induction solution and 50% PRF. The expression of differences in cell proliferation, alkalinephosp-hatase (ALP) activity, inflammatory factors and key factors of Wnt/β-catenin signaling pathway were compared among all groups. Results: (1) Compared with the other 3 groups, the absorbance value (OD) of intervention group 1 significantly increased, compared with TNF-α group, the OD of intervention group 2 and control group significantly increased (P<0.05). (2) Compared with the other 3 groups, the ALP activity of intervention group 1 significantly increased. Compared with TNF-α group, the ALP activity of intervention group 2 and control group significantly increased (P<0.05). (3) Compared with the other 3 groups, the expression levels of bonemorphogeneticprotein-2 (BMP2) mRNA, runt-relatedtranscriptionfactor-2 (Runx2) mRNA, BMP2 and Runx2 protein of intervention group 1 significantly increased, compared with TNF-α group, the expression levels of BMP2 mRNA, Runx2 mRNA, BMP2 and Runx2 protein in intervention group 2 and control group significantly increased (P<0.05). (4) Compared with intervention group 1 and control group, the expression level of TNF-α mRNA of intervention group 2 and TNF-α group significantly increased (P<0.05). (5) compared with the other 3 groups, the expression levels of CyclinD1 mRNA and β-catenin mRNA of intervention group 1 significantly increased, and compared with TNF-α group, the expression levels of CyclinD1 mRNA and β-catenin mRNA of intervention group 2 and control group significantly increased (P<0.05). Conclusion: PRF can promote the proliferation and osteogenesis of hPDLCs cells, reduce the expression of inflammatory factors, and enhance the ALP activity, which may promote the expression of CyclinD1 and β-catenin mRNA and accelerate the osteogenesis of hPDLCs cells by activating the Wnt/β-catenin signaling pathway. |
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