| 王 超,安瑞芳,何菊仙,白昌民,赵 萌.GnRH类似物对子宫肌瘤大鼠模型血液黏度、子宫系数和炎性细胞浸润的影响[J].现代生物医学进展英文版,2022,(4):632-636. |
| GnRH类似物对子宫肌瘤大鼠模型血液黏度、子宫系数和炎性细胞浸润的影响 |
| Effects of GnRH Analogues on Blood Viscosity, Uterine Coefficient and Inflammatory Cell Infiltration in Rat Models of Uterine Fibroids |
| Received:July 06, 2021 Revised:July 31, 2021 |
| DOI：10.13241/j.cnki.pmb.2022.04.007 |
| 中文关键词: 促性腺激素释放激素 子宫肌瘤 大鼠 血液黏度 子宫系数 炎性细胞浸润 |
| 英文关键词: Gonadotropin releasing hormone Uterine fibroids rats Blood viscosity Uterine coefficient Inflammatory cell Infiltration |
| 基金项目:陕西省卫健委科研项目（2018D036） |
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| 中文摘要: |
| 摘要 目的：探讨促性腺激素释放激素（Gonadotropin releasing hormone，GnRH）类似物对子宫肌瘤大鼠模型血液黏度、子宫系数和炎性细胞浸润的影响。方法：子宫肌瘤大鼠模型（n=36）随机平分为三组-模型组、米非司酮组与GnRH类似物组，分别给予腹腔注射0.15 ml的生理盐水、2 mg/kg米非司酮与2 mg/kg GnRH类似物，每周1次，持续8周。结果：米非司酮组与GnRH类似物组治疗第4周与第8周的全血比黏度、卵泡刺激素（FSH）、黄体生成素（LH）值都低于模型组（P<0.05），GnRH类似物组低于米非司酮组（P<0.05）。米非司酮组与GnRH类似物组治疗第8周的子宫系数、子宫白介素（IL）-10与肿瘤坏死因子（TNF）-α表达水平都低于模型组（P<0.05），GnRH类似物组低于米非司酮组（P<0.05）。米非司酮组与GnRH类似物组治疗第8周的子宫内膜厚度、腺间质面积比、腺体面积与腺腔面积都高于模型组（P<0.05），GnRH类似物组高于米非司酮组（P<0.05）。米非司酮组与GnRH类似物组治疗第8周的子宫组织Wnt5b与β-catenin蛋白相对表达水平低于模型组（P<0.05），GnRH类似物组低于米非司酮组（P<0.05）。结论：GnRH类似物在子宫肌瘤大鼠模型的应用能降低子血液黏度，还可抑制血清性激素的分泌，增加子宫系数，抑制Wnt5b与β-catenin蛋白的表达，从而可改善子宫肌瘤大鼠的炎性细胞浸润状态与子宫内膜形态。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of Gonadotropin releasing hormone (GnRH) analogues on blood viscosity, uterine coefficient and inflammatory cell infiltration in rat models of uterine fibroids. Methods: Rat models of uterine fibroids (n=36) were equally randomly divided into three groups-model group, mifepristone group and GnRH analogue group. The three groups were given intraperitoneal injection of 0.15 mL of normal saline, 2 mg/kg of mifepristone, 2 mg/kg GnRH analogue, once a week for 8 weeks. Results: The values of whole blood specific viscosity, follicle stimulating hormone (FSH) and luteinizing hormone (LH) in the 4th and 8th weeks of treatment in the mifepristone group and GnRH analog group were lower than those of the model group (P<0.05), the GnRH analog group were lower than mifepristone group (P<0.05). Uterine coefficient, uterine interleukin (IL)-10 and tumor necrosis factor (TNF)-α expression levels in the mifepristone group and GnRH analog group at the 8th week of treatment were lower than those in the model group (P<0.05), GnRH analog The group were lower than the mifepristone group (P<0.05). The endometrial thickness, gland-to- interstitial area ratio, gland area and gland cavity area of the mifepristone group and the GnRH analog group were higher than the model group at the 8th week of treatment (P<0.05), and the GnRH analog group were higher than that in the GnRH analog group Mifepristone group (P<0.05). The relative expression levels of Wnt5b and β-catenin protein in the uterine tissue of the mifepristone group and the GnRH analog group were lower than the model group at the 8th week of treatment (P<0.05), and the GnRH analog group were lower than the mifepristone group (P< 0.05). Conclusion: The application of GnRH analogues in rat models of uterine fibroids can reduce blood viscosity, inhibit the secretion of serum sex hormones, increase uterine coefficient, and inhibit the expression of Wnt5b and β-catenin proteins, thereby improving morphology of the endometrium uterine fibroids and the infiltration state of inflammatory cells in rats . |
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