| 李景磊,辛 茜,马 龙,卜国森,周 钰.基于JAK2/STAT3信号通路探讨头针结合艾灸对缺血缺氧脑瘫幼鼠模型的影响及作用机制[J].现代生物医学进展英文版,2022,(3):427-431. |
| 基于JAK2/STAT3信号通路探讨头针结合艾灸对缺血缺氧脑瘫幼鼠模型的影响及作用机制 |
| Based on JAK2-STAT3 Signal Pathway to Explore the Effect and Action Mechanism of Scalp Acupuncture Combined with Moxibustion on the Young Rats Model of Ischemic-Hypoxic Cerebral Palsy |
| Received:May 27, 2021 Revised:June 23, 2021 |
| DOI：10.13241/j.cnki.pmb.2022.03.006 |
| 中文关键词: 缺血缺氧脑瘫 幼鼠 头针 艾灸 JAK2/STAT3 |
| 英文关键词: Ischemic-hypoxic cerebral palsy Young rats Scalp acupuncture Moxibustion JAK2/STAT3 |
| 基金项目:国家自然科学基金项目（81960356） |
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| 中文摘要: |
| 摘要 目的：基于JAK2/STAT3信号通路探讨头针结合艾灸对缺血缺氧脑瘫幼鼠模型的影响及作用机制。方法：选取40只7 d龄的清洁级SD幼鼠，按随机数字表法分为A组、B组、C组、D组、E组，每组8只。E组不予以任何处理，其余各组幼鼠均以改良的缺氧缺血性脑病造模方法构建缺血缺氧脑瘫模型。造模成功后A组、B组、C组分别给予头针结合艾灸治疗、头针治疗、艾灸治疗，D组、E组均给予50 ?滋L生理盐水。对比各组幼鼠脑组织Janus蛋白酪氨酸激酶2（JAK2）及转录活化因子3（STAT3）蛋白表达量、血清炎症因子[白介素-6（IL-6）、肿瘤坏死因子--α（TNF-α）、白介素-1（IL-1）]水平、神经递质[去甲肾上腺素（NE）、5-羟色胺（5-HT）]水平、记忆功能。结果：A组脑组织JAK2、STAT3 蛋白表达量低于B、C、D组，但高于E组（P<0.05）；A组血清IL-6、IL-1和TNF-α水平低于B、C、D组，但高于E组（P<0.05）；A组血清NE、5-HT水平低于B、C、D组，但高于E组（P<0.05）；A组Y迷宫实验正确次数多于B、C、D组（P<0.05），而少于E组（P<0.05）。结论：头针结合艾灸可通过靶向抑制JAK2/STAT3信号通路相关蛋白的表达下调机体炎症反应水平，从而降低缺血缺氧脑瘫幼鼠脑组织损伤，促进其记忆功能恢复。 |
| 英文摘要: |
| ABSTRACT Objective: Based on the JAK2/STAT3 signaling pathway, to explore the effect of scalp acupuncture combined with moxibustion on a rat model of ischemic-hypoxic cerebral palsy and its mechanism. Methods: Selected 40 7-day-old clean-grade SD young rats and divide them into group A, group B, group C, group D, group E according to the random number table method, with 8 in each group. No treatment was given to the group E, and the young rats of the other groups were all constructed models of ischemic-hypoxic cerebral palsy using the modified hypoxic-ischemic encephalopathy modeling method. After successful model building, groups A, B, and C were given scalp acupuncture combined with moxibustion, scalp acupuncture, and moxibustion respectively. Groups D and E were given 50 μL of normal saline. To compare the expression of Janus protein tyrosine kinase 2 (JAK2) and activator of transcription 3 (STAT3) protein, serum inflammatory factors [interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1)] level, neurotransmitter [norepinephrine (NE), serotonin (5-HT)] level, memory function among young rats of each group. Results: The expression of JAK2 and STAT3 protein in the brain tissue of group A was lower than that of groups B, C, D, but higher than that of group E (P<0.05). Serum IL-6, IL-1 and TNF-α levels of group A were lower than groups B , C, D, but higher than group E(P<0.05). Serum NE and 5-HT levels in group A were lower than those in groups B, C and D, but higher than those in group E(P<0.05). Group A has more correct Y maze experiments than groups B, C, and D (P<0.05), but less than group E(P<0.05). Conclusion: Scalp acupuncture combined with moxibustion can down-regulate the inflammatory response level by targeting the expression of related proteins in the JAK2/STAT3 signaling pathway, thereby reducing brain tissue damage in young rats with ischemic-hypoxic cerebral palsy and promoting the recovery of their memory function. |
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