| 郭 媛,孙大燕,李 轶,郝 萌,王久存.1-磷酸鞘氨醇改善缺氧诱导的肺上皮细胞损伤[J].现代生物医学进展英文版,2022,(1):1-5. |
| 1-磷酸鞘氨醇改善缺氧诱导的肺上皮细胞损伤 |
| Protective Effect of Sphingominol 1-phosphate for Hypoxia-induced Lung Epithelial Cells Injury |
| Received:May 30, 2021 Revised:June 27, 2021 |
| DOI：10.13241/j.cnki.pmb.2022.01.001 |
| 中文关键词: 1-磷酸鞘氨醇 低氧暴露 肺上皮细胞 线粒体 氧化损伤 |
| 英文关键词: S1P Hypoxia Lung epithelial cells Mitochondrion Oxidative Stress |
| 基金项目:上海市科技重大专项 （2017SHZDZX01） |
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| 中文摘要: |
| 摘要 目的：如何减轻缺氧造成的肺损伤是平原人群进入高原环境时面临的难题。本研究旨在探索外源性1-磷酸鞘氨醇（S1P）对低氧暴露诱导肺上皮细胞损伤的改善作用。方法：对肺上皮细胞（BEAS 2B细胞）进行4 h不同浓度的S1P预处理，之后放入低氧培养箱（氧气浓度为1%）模拟24 h和48 h的低氧暴露，检测细胞的增殖活性、早期凋亡以及线粒体相关功能；通过实时荧光定量PCR检测受体基因（S1PR1-3）的表达水平。结果：外源性S1P预处理可在BEAS 2B细胞中显著提高S1PR3的表达水平；对于24 h - 48 h的急性低氧暴露，给予1 μM浓度的S1P预处理时对细胞具有显著的保护作用，主要表现在线粒体功能改善、细胞增殖活性提升及早期凋亡率下降，包括：线粒体膜电位（MMP）和三磷酸腺苷（ATP）水平显著升高（P＜0.0005），线粒体活性氧（ROS）产生显著减少（P＜0.0001），从而显著提高了细胞的增殖活性（P＜0.005），并降低早期凋亡率。结论：外源性S1P预处理能通过改善低氧诱导的氧化应激损伤保护肺上皮细胞。S1P在预防急性高原病、改善高原反应方面具有潜在应用价值。 |
| 英文摘要: |
| ABSTRACT Objective: How to reduce the lung ingury caused by hypoxia is a challenge when people enter the high altitude. The purpose of this study is to explore the protective effect of exogenous sphingosinol 1-phosphate (S1P) on lung epithelial cells during hypoxic exposure. Methods: BEAS 2B cells were pretreated with S1P at different concentrations for 4 hours and then placed in a hypoxia incubator (1% oxygen concentration) to simulate hypoxia exposure during 24 h and 48 h, and then detect the proliferative activity，early apoptosis and mitochondria-related functions; in addition, the expression level of the receptor genes(S1PR1-3) were tested by real-time qPCR. Results: Exogenous S1P preconditioning can increase the expression level of S1PR3 significantly in BEAS 2B; During 24 h - 48 h of hypoxia exposure, S1P pretreatment at 1 μM had a significant protective effect on BEAS 2B, especially improving Mitochondrial Membrane Potential(MMP) and Adenosine Triphosphate(ATP) levels(P＜0.0005), reducing Reactive Oxygen Species(ROS) production(P＜0.0001), Thus, increase the proliferation activity(P＜0.005) and reduce the early apoptosis rate of cells. Conclusion: Exogenous S1P preconditioning can protect lung epithelial cells by inhibition of hypoxia-induced oxidative stress injury. S1P has important application value in preventing altitude sickness. |
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