| 陈燕伟,武秀权,王 利,李 侠,林 伟,蒋晓帆,张 磊.维生素C对创伤性脑损伤后神经细胞焦亡及NLRP3通路的影响[J].现代生物医学进展英文版,2021,(24):4632-4636. |
| 维生素C对创伤性脑损伤后神经细胞焦亡及NLRP3通路的影响 |
| Effects of Vitamin C on Nerve Cell Pyrolysis and NLRP3 Pathway Following with Traumatic Brain Injury |
| Received:March 23, 2021 Revised:April 18, 2021 |
| DOI：10.13241/j.cnki.pmb.2021.24.006 |
| 中文关键词: 维生素C Nod 样受体蛋白3 创伤性脑损伤 白介素-1β 白介素-18 |
| 英文关键词: Vitamin C Nod-likereceptor family pyrin domain containing 3 Traumatic brain injury Interleukin -1β Interleukin -18 |
| 基金项目:国家自然科学基金面上项目（81974188）；陕西省国际科技合作计划项目（2019KW-039）；陕西省重点研发计划项目（2018SF-154） |
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| 中文摘要: |
| 摘要 目的：考察创伤性脑损伤（TBI）中维生素C（Vit C）预处理抗神经细胞焦亡的作用和分子机制。方法：培养HT22细胞系，随机分为空白组（Sham组）、DMSO组（Con组）和Vit C预处理组（Vit C组），Vit C组分为低剂量和高剂量2组。Vit C组和Con组进行预处理72 h后划伤12 h，制成神经细胞创伤性脑损伤模型。利用荧光免疫组化法和Western-blot检测NLRP3的表达变化；标准ELISA试剂盒检测IL-1β和 IL-18表达变化。结果：相较于Sham组，Con组和Vit C组中HT22细胞中NLRP3的染色阳性率高；与Con组比较，Vit C组的NLRP3表达明显减少(P<0.05)，且IL-1β和 IL-18表达也显著降低(P<0.05)，各组间差异有统计学意义(P<0.05)。结论：TBI后利用不同剂量Vit C均可有效降低神经细胞焦亡，可能是通过下调NLRP3表达水平，减少IL-1β和 IL-18等释放，发挥神经保护作用。 |
| 英文摘要: |
| ABSTRACT Objective: To observed the effect and molecular mechanism of vitamin C(Vit C) pretreatment against nerve cell pyroptosis in traumatic brain injury (TBI). Methods: The HT22 cell lines were randomly divided into the sham group (sham group), DMEM control group (Con group) and the Vit C pretreatment group (Vit C group); Con group and Vit C group pretreatment in 72 h, then the TBI model was induced by scratch injury in the groups for 12 h and then sampling. The expression of NLRP3 was detected by immunofluorescence staining and Western-blot; The standard ELISA kit was used to detect the expression of IL-1β and IL-18. Results: Compared with the Sham group, the positive rate of NLRP3 staining in surviving neurons was high in the Con group and the Vit C groups; Compared with Con group, low and high dose Vit C can obviously reduce the expression level of NLRP3 protein, IL-1β and IL-18(P<0.05), and the difference among the low dose and the high dose groups was statistically significant(P<0.05). Conclusion: Different doses of Vit C effectively reduce nerve cell pyroptosis after TBI, possibly by alleviating the expression level of NLRP3, reducing the release of IL-1β and IL-18, and play important neuroprotective effects. |
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