| 王佳丽,邹 龑,陈淑萍,吴 军,邓晓隽.脓毒症大鼠肝肾功能、凝血功能和炎症指标的变化及意义[J].现代生物医学进展英文版,2021,(24):4628-4631. |
| 脓毒症大鼠肝肾功能、凝血功能和炎症指标的变化及意义 |
| Changes and Significance of Liver and Kidney Function, Coagulation Function and Inflammation Indexes in Sepsis Rats |
| Received:April 28, 2021 Revised:May 23, 2021 |
| DOI：10.13241/j.cnki.pmb.2021.24.005 |
| 中文关键词: 脓毒症 肝肾功能 凝血功能 炎症指标 |
| 英文关键词: Sepsis Liver and kidney function Coagulation function Inflammation index |
| 基金项目:上海市第六人民医院东院院级科学研究基金（DY2018011）；上海市卫生健康委员会科研项目计划（202040341） |
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| 中文摘要: |
| 摘要 目的：研究脓毒症大鼠肝肾功能、凝血功能和炎症指标的变化及意义。方法：选取SPF级雄性SD大鼠20只，以随机数字表法将其分成实验组与假手术组，每组各10只。其中实验组通过盲肠穿刺结扎制备脓毒症大鼠模型，假手术组大鼠仅翻动盲肠，不作结扎处理。分别采集所有大鼠术后0 h、6 h、12 h、24 h时的尾静脉血3 mL，检测并比较上述时间点两组大鼠的肝肾功能、凝血功能和炎症指标水平的差异。结果：实验组术后6 h、12 h、24 h的谷丙转氨酶（ALT）、尿素氮（BUN）以及肌酐（Cr）水平均高于假手术组（P＜0.05）。实验组术后24 h的活化部分凝血活酶时间（APTT）、凝血酶原时间（PT）、纤维蛋白原（FIB）水平均高于假手术组（P＜0.05）。实验组大鼠术后6 h、12 h、24 h的血清白细胞计数（WBC）、白细胞介素-6（IL-6）、降钙素原（PCT）水平均高于假手术组（P＜0.05）。结论：脓毒症大鼠肝肾功能存在明显的受损，凝血功能障碍明显，且机体内会释放大量的炎症因子。 |
| 英文摘要: |
| ABSTRACT Objective: To study the changes and significance of liver and kidney function, coagulation function and inflammation indexes in sepsis rats. Methods: 20 male SPF grade SD rats were selected, and they were randomly divided into experimental group and sham operation group by random number table method, with 10 rats in each group. Sepsis rats in the experimental group were made by cecal puncture and ligation, while rats in the sham operation group were only turned over the cecum without ligation. 3 mL of tail venous blood of all rats at 0 h, 6 h, 12 h and 24 h after surgery was collected respectively, the differences in liver and kidney function, blood coagulation function and inflammation index levels between the two groups at the above time points were detected and compared. Results: The levels of alanine aminotransferase (ALT), urea nitrogen (BUN) and creatinine (Cr) in experimental group at 6 h, 12 h and 24 h after surgery were higher than those in sham operation group(P＜0.05). The levels of activated partial thromboplastin time (APTT), prothrombin time (PT) and fibrinogen (FIB) in experimental group at 24 h after surgery were higher than those in sham operation group (P＜0.05). The levels of serum white blood cell count (WBC), interleukin-6 (IL-6) and procalcitonin (PCT) in the experimental group at 6 h, 12 h and 24 h after surgery were higher than those in the sham operation group(P＜0.05). Conclusion: The liver and kidney functions of sepsis rats are obviously impaired, and the coagulation dysfunction is obvious, and a large amount of inflammatory transmitters are released in the body. |
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