| 余海静,汪 琳,张 丹,王志斌,刘 阳.IgA肾病患者扁桃体中树突状细胞和高内皮小静脉关系的研究[J].现代生物医学进展英文版,2021,(23):4450-4453. |
| IgA肾病患者扁桃体中树突状细胞和高内皮小静脉关系的研究 |
| The Relationship between Dendritic Cells and High Endothelial Venules in Tonsils of Patients with IgA Nephropathy |
| Received:January 16, 2021 Revised:February 14, 2021 |
| DOI：10.13241/j.cnki.pmb.2021.23.010 |
| 中文关键词: 树突状细胞 IgA肾病 扁桃体切除术 |
| 英文关键词: Dendritic cells IgA nephropathy Tonsillectomy |
| 基金项目:国家自然科学基金项目（81770982；81300812） |
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| 中文摘要: |
| 摘要 目的：探讨IgA肾病（IgA nephropathy，IgAN）患者扁桃体树突状细胞（dendritic cells，DCs）与高内皮小静脉(High endothelial venules, HEVs)的关系。方法：我们检测了25例IgAN患者的扁桃体，并以复发性扁桃体炎（Recurrent tonsilitis, RT）患者的扁桃体22例作为对照。用免疫组化方法检测DCs标志物CD208，用实时聚合酶链反应检测淋巴毒素β（ Lymphotoxin β，LT-β） mRNA水平，用Hematoxylin-eosin染色确定HEVs。结果：IgAN患者扁桃体CD208+DCs明显高于对照组（P<0.001）。IgAN患者扁桃体HEVs明显高于对照组（P<0.001）。此外， IgAN患者扁桃体LT-mRNA水平明显高于对照组（P<0.001）。IgAN 扁桃体中CD208+DC与HEVs明显成正相关（r=0.7427，P<0.001)。结论：扁桃体CD208+DCs可能通过HEVs导致IgAN患者扁桃体免疫的异常而促进肾脏疾病的发展。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the relationship between tonsil dendritic cells（DCs） and high endothelial venules (HEVs) in tonsils of patients with IgA nephropathy (IgAN). Methods: The tonsils of 25 patients with IgAN and 22 patients with recurrent tonsilitis（RT）were examined and compared. The DC marker CD208 was analyzed by immunohistochemistry. The level of lymphotoxin β (LT-β) mRNA was detected by real-time polymerase chain reaction. Hematoxylin-eosin staining was used to identify HEVs. Results: CD208 + DCs of tonsils in IgAN patients was significantly higher than that in the control group（P<0.001）. The HEVs of tonsils in IgAN patients were significantly higher than those in the control group（P<0.001）. In addition, the level of LT-β mRNA in patients with IgAN was significantly higher than that in the control group（P<0.001）. CD208 + DC was positively correlated with HEVs in tonsils of IgAN patients（r=0.7427, P<0.001). Conclusion: CD208 + DCS in tonsil may play a role in the pathogenesis of IgAN through HEVs. The results of this study support the therapeutic significance of tonsillectomy in patients with IgAN. |
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