| 袁瑞丽,柳 娟,刘 妮,赵 娜,尹晶晶,郭 炫.miR-134-5p靶向抑制PAK3表达增强多发性骨髓瘤细胞的化疗敏感性的研究[J].现代生物医学进展英文版,2021,(22):4229-4233. |
| miR-134-5p靶向抑制PAK3表达增强多发性骨髓瘤细胞的化疗敏感性的研究 |
| Study of miR-134-5p Enhances Chemosensitivity in Multiple Myeloma Cells by Inhibiting Expression of PAK3 |
| Received:March 04, 2021 Revised:March 28, 2021 |
| DOI：10.13241/j.cnki.pmb.2021.22.006 |
| 中文关键词: 多发性骨髓瘤 化疗敏感性 miR-134-5p p21活化激酶3 |
| 英文关键词: Multiple myeloma Chemosensitivity miR-134-5p p21 activated kinase 3 |
| 基金项目:陕西省国际科技合作交流计划项目（2016KW-007） |
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| 中文摘要: |
| 摘要 目的：研究miR-134-5p对多发性骨髓瘤（multiple myeloma, MM）化疗敏感性的影响及其可能的作用机制。方法：CCK-8法测定人多发性骨髓瘤细胞KM3及其耐硼替佐米（bortezomib, BTZ）细胞株KM3/BTZ对BTZ的化疗敏感性，RT-PCR法检测KM3和KM3/BTZ细胞株中miR-134-5p和p21活化激酶3（p21 activated kinase 3, PAK3）mRNA的表达，生物信息学软件分析miR-134-5p的靶基因，荧光素酶报告实验进行验证，CCK-8法检测分别抑制miR-134-5p和PAK3后KM3/BTZ的化疗敏感性，Western-blot法检测抑制miR-134-5p后KM3/BTZ细胞株中PAK3蛋白的表达。结果：KM3/BTZ细胞株对BTZ的化疗敏感性显著低于KM3细胞株（P＜0.05）。MiR-134-5p在KM3细胞株的表达显著高于KM3/BTZ细胞株，而PAK3 mRNA在KM3细胞株的表达显著低于KM3/BTZ细胞株（P＜0.05）。PAK3为miR-134-5p的靶基因。MiR-134-5p inhibitor组和PAK3 siRNA组KM3/BTZ细胞株的化疗敏感性显著低于对照组（P＜0.05）。MiR-134-5p inhibitor组PAK3蛋白的相对表达显著高于对照组（P＜0.05）。结论：MiR-134-5p可提高KM3/BTZ细胞株的化疗敏感性，其机制可能与抑制PAK3的表达有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of miR-134-5p on chemosensitivity in multiple myeloma(MM) cells and its potential mechanism. Methods: The chemosensitivity of KM3 and KM3/BTZ cells were detected by CCK-8 method, the expression of miR-134-5p and p21 activated kinase 3(PAK3) mRNA in KM3 and KM3/BTZ cells were detected by RT-PCR, bioinformatics prediction software was used to predict potential target gene to miR-134-5p, and luciferase report experiment was used to verified, the chemosensitivity of KM3 and KM3/BTZ cells after inhibiting miR-134-5p and PAK3 were detected by CCK-8 method, the expression of PAK3 protein in KM3/BTZ cell after inhibiting miR-134-5p were detected by western-blot. Results: The chemosensitivity of KM3/BTZ cell was lower than KM3 cell(P＜0.05). The expression of miR-134-5p in KM3 cell was higher than KM3/BTZ, while PAK3 mRNA was lower(P＜0.05). PAK3 was a target gene of miR-134-5p. The chemosensitivity of KM3/BTZ cell of miR-134-5p inhibitor group and PAK3 siRNA group were lower than control group(P＜0.05). The expression of PAK3 protein in miR-134-5p inhibitor group was higher than control group(P＜0.05). Conclusion: MiR-134-5p could increase the chemosensitivity of KM3/BTZ cell, the potential mechanism might be via inhibiting expression of PAK3. |
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