| 陈志强,陈永杰,段兴邦,邵 帅,李 利.miRNA-125a在脑卒中的作用及其机制研究[J].现代生物医学进展英文版,2021,(19):3685-3689. |
| miRNA-125a在脑卒中的作用及其机制研究 |
| The Role and Mechanism of miRNA-125a in Stroke |
| Received:March 27, 2021 Revised:April 23, 2021 |
| DOI：10.13241/j.cnki.pmb.2021.19.017 |
| 中文关键词: 缺血性卒中 miR-125a 水通道蛋白4 |
| 英文关键词: Stroke miR-125a Aquaporin 4 |
| 基金项目:哈尔滨市科技创新人才项目（2017RAQXJ173） |
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| 中文摘要: |
| 摘要 目的：探究miR-125a在脑卒中的作用及其分子机制，为miR-125a在脑缺血治疗中的应用提供理论基础。方法：收集正常和脑卒中患者的血浆，Realtime PCR检测miR-125a的表达水平，ROC曲线分析敏感性和特异性。Person相关性分析miR-125a表达水平与患者NIHSS评分和脑梗死体积的相关性。Realtime PCR检测MCAO模型大鼠脑皮质和血浆中miR-125a表达水平，并进行相关性分析。9.4T磁共振测量MCAO模型大鼠脑梗死体积。Realtime PCR和Western Blot法检测AQP4 mRNA和蛋白的表达水平。结果：研究一共收集到70例正常和50例缺血性脑卒中患者的血浆样本，两组在性别（χ2 = 1.469，P = 0.225）和年龄（Z = -0.208，P = 0.835）上无差异。缺血性脑卒中患者血浆中miR-125a表达水平高于正常组（Z = -7.01，P = 0.000）。预后良好患者血浆中miR-125a表达水平低于预后不良患者（Z = -2.183，P = 0.029）。血浆中miR-125a表达水平在区分正常者和缺血性脑卒中患者的敏感性和特异性较好（ROC = 0.876，P = 0.000）。血浆中miR-125a表达水平与患者NIHSS评分正相关（r = 0.303，P = 0.032），和脑梗死体积正相关性（r = 0.399，P = 0.004）。MCAO大鼠脑皮质中miR-125a表达水平升高（t = 8.918，P = 0.000），血浆中miR-125a表达水平升高（t = 4.928，P = 0.000），脑皮质与血浆中miR-125a表达水平呈正相关（r = 0.823，P = 0.044）。第3天时，sh-miR-125a组脑梗死体积小于NC组。sh-miR-125a组AQP4 mRNA和蛋白表达水平小于NC组。结论：血浆中miR-125a表达水平在区分正常和脑卒中患者具有较好的敏感性和特异性，抑制miR-125a表达会通过降低AQP4的表达，减少脑梗死体积，为miR-125a在诊断和治疗脑缺血中的应用提供了理论基础。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the role and mechanism of miR-125a in stroke, and provide a theoretical basis for the application of miR-125a in the treatment of cerebral ischemia. Methods: Collected the plasma of normal and stroke patients, detected the expression of miR-125a by Realtime PCR, and analyze the sensitivity and specificity of ROC curve. Person correlation analyze the correlation between the miR-125a level and patient's NIHSS score, and cerebral infarction volume. Realtime PCR detected the expression of miR-125a in the cerebral cortex and plasma of MCAO model rats, and analyze the correlation. 9.4T magnetic resonance measurement of cerebral infarction volume in MCAO model rats. Realtime PCR and Western Blot methods were used to detect the expression of AQP4 mRNA and protein. Results: 70 normal and 50 ischemic stroke patients' plasma samples were collected in this study. There was no difference in gender (χ2 = 1.469, P = 0.225) and age (Z = -0.208, P = 0.835) in the two groups. The expression of miR-125a in the plasma of ischemic stroke patients was higher than that in the normal group (Z = -7.01, P = 0.000). The expression of miR-125a in the plasma of patients with good prognosis was lower than that of patients with poor prognosis (Z = -2.183, P = 0.029). The expression of miR-125a in plasma has good sensitivity and specificity in distinguishing normal patients from ischemic stroke patients (ROC = 0.876, P = 0.000). The expression of miR-125a in plasma was positively correlated with the patient's NIHSS score (r = 0.303, P = 0.032), and positively correlated with cerebral infarction volume (r = 0.399, P = 0.004). The expression of miR-125a in the cerebral cortex of MCAO rats increased (t = 8.918, P = 0.000), the expression level of miR-125a in the plasma increased (t = 4.928, P = 0.000), and the level of miR-125a in the cerebral cortex and plasma was increased and they were positively correlated (r = 0.823, P = 0.044). On the 3rd day, the cerebral infarction volume of the miR-125a knockdown group was smaller than the blank control group. The mRNA and protein expression of AQP4 in the miR-125a knockdown group were lower than the blank control group. Conclusion: The expression of miR-125a in plasma has good sensitivity and specificity in distinguishing between normal and stroke patients. Inhibition of miR-125a expression will reduce the expression of AQP4 and the volume of cerebral infarction, which provides a theoretical basis for miR-125a in diagnosis and treatment of cerebral ischemia. |
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