| 顾亚静,李蓉蓉,陈培培,李 悦,李 莉.肠内营养联合益生菌对直肠恶性肿瘤术后大鼠肠粘膜屏障及血清炎症因子的影响[J].现代生物医学进展英文版,2021,(19):3628-3632. |
| 肠内营养联合益生菌对直肠恶性肿瘤术后大鼠肠粘膜屏障及血清炎症因子的影响 |
| Effect of Enteral Nutrition Combined with Probiotics on Intestinal Mucosal Barrier and Serum Inflammatory Factors of Rats after Rectal Cancer Operation |
| Received:March 03, 2021 Revised:March 27, 2021 |
| DOI：10.13241/j.cnki.pmb.2021.19.006 |
| 中文关键词: 肠内营养 益生菌 直肠恶性肿瘤 肠粘膜屏障 炎症因子 |
| 英文关键词: Enteral nutrition Probiotics Cancer of rectum Intestinal mucosal barrier Inflammatory factors |
| 基金项目:国家自然科学基金项目（81960145） |
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| 中文摘要: |
| 摘要 目的：研究肠内营养（enteral nutrition, EN）联合益生菌对直肠恶性肿瘤术后大鼠肠粘膜屏障及血清炎症因子的影响。方法：选取32只SPF级雄性SD大鼠，随机分对照组、模型组、EN组和EC组，每组8只。模型组、EN组和EC组建立直肠恶性肿瘤术后模型，EN组灌胃给予肠内营养混悬液，EC组灌胃肠内营养混悬液加益生菌颗粒，对照组和模型组灌胃同体积的生理盐水，四组大鼠均连续灌胃7 d。观察各组大鼠小肠超微结构；检测occludin蛋白、D-乳糖及炎症因子的表达水平。结果：模型组大鼠小肠上皮细胞紧密连接和绒毛结构遭破坏，EN组和EC组与模型组相比有明显改善。与对照组相比，模型组大鼠occludin蛋白和抑炎因子IL-10的表达水平均较低，D-乳糖、血清白介素1β（interleukin-1β, IL-1β）和肿瘤坏死因子-α（tumor necrosis factor-α,TNF-α）的表达水平较高（P＜0.05）；与模型组相比，EN组和EC组的occludin蛋白和IL-10的表达水平较高，D-乳糖、IL-1β和TNF-α的表达水平较低（P＜0.05）；与EN组相比，EC组occludin蛋白和IL-10的表达水平较高，D-乳糖、IL-1β和TNF-α的表达水平较低（P＜0.05）。结论：肠内营养联合益生菌可有效改善直肠恶性肿瘤术后大鼠的肠道屏障功能，提高occludin蛋白的表达，降低D-乳糖的表达，降低促炎因子IL-1β和TNF-α的表达同时升高抑炎因子IL-10的表达。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effect of enteral nutrition(EN) combined with probiotics on intestinal mucosal barrier and serum inflammatory factors of rats after rectal cancer operation. Methods: 32 male SPF grade SD rats were randomly divided into control group, model group, EN group and EC group, with 8 rats in each group. The model group, EN group and EC group were established postoperative rectal cancer model, the EN group was administrated with enteral nutritional suspension, the EC group was administrated with enteral nutritional suspension combined with probiotic granules, the control group and model group were administrated with same volume of saline. The ultrastructure of small intestine of each group was observed, the occludin protein, D-lactose and inflammatory factors were detected. Results: The tight junction and villi structure of intestinal epithelial cells of model group were disorganized, while EN group and EC group were improved. As compared with control group, the expression of occludin protein and IL-10 of model group were lower, while D-lactose, IL-1β and TNF-α were higher(P<0.05); as compared with model group, the expression of occludin protein and IL-10 of EN group and EC group were higher, while D-lactose, IL-1β and TNF-α were lowedr(P<0.05); as compared with EN group, the expression of occludin protein and IL-10 of EC group were higher, while D-lactose, IL-1β and TNF-α were lowedr(P<0.05). Conclusion: EN combined with probiotics could siginificantly alleviate the intestinal mucosal barrier function, increase the expression of occludin protein and decrease the expression of D-lactose, decrease the expression of IL-1β and TNF-α while increase the expression of IL-10. |
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