| 高 进,杨晓军,高淑靖,邱国宇,曹凤波.中药止血粉联合止血后口服桃仁承气汤对上消化道出血小鼠的治疗效果及相关机制研究[J].现代生物医学进展英文版,2021,(19):3612-3616. |
| 中药止血粉联合止血后口服桃仁承气汤对上消化道出血小鼠的治疗效果及相关机制研究 |
| Study on Therapeutic Effect and Related Mechanisms of Chinese Medicine Hemostatic Powder Combined with Hemostasis and Oral Administration of Taoren Chengqi Decoction on Mice with Upper Gastrointestinal Hemorrhage |
| Received:May 07, 2021 Revised:May 27, 2021 |
| DOI：10.13241/j.cnki.pmb.2021.19.003 |
| 中文关键词: 中药止血粉 桃仁承气汤 上消化道出血 小鼠 核因子κB 白介素-6 |
| 英文关键词: Traditional Chinese Medicine Hemostatic Powder Taoren Chengqi Decoction Upper Gastrointestinal Bleeding Mice Nuclear Factor κB Interleukin-6 |
| 基金项目:国家中医药管理局科研项目（国中医药人教函〔2012〕149号）；广东省教育厅项目（2016SFKC_09） |
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| 中文摘要: |
| 摘要 目的：探讨与研究中药止血粉联合止血后口服桃仁承气汤对上消化道出血小鼠的治疗效果及相关机制。方法：C57野生型雄性小鼠24只随机平分为三组-模型组、止血组、联合组，三组建立了应激性消化道出血模型。建模后2周联合组给予中药止血粉联合口服桃仁承气汤治疗，止血组给予中药止血粉治疗，模型组给予等剂量的生理盐水治疗。结果：实验过程中所有小鼠都存活，止血组、联合组治疗第2周与第4周的体重高于模型组(P<0.05)，联合组高于止血组(P<0.05)。止血组、联合组治疗第2周与第4周的血清白介素(Interleukin，IL)-6与IL-8表达水平低于模型组(P<0.05)，联合组低于止血组(P<0.05)。止血组、联合组治疗第2周与第4周的消化道溃疡出血指数对比都低于模型组(P<0.05)，联合组低于止血组(P<0.05)。止血组、联合组治疗第2周与第4周的胃上皮组织核因子κB(nuclear factor kappa-B，NF-κB)、Caspase-3蛋白相对表达水平低于模型组(P<0.05)，联合组低于止血组(P<0.05)。结论：中药止血粉联合止血后口服桃仁承气汤在应激性消化道出血小鼠中的应用能减轻消化道组织损伤，抑制炎症因子的表达，促进小鼠正常发育，其作用机制可能与抑制NF-κB、Caspase-3蛋白表达有关。 |
| 英文摘要: |
| ABSTRACT Objective: To explore and study the therapeutic effects and related mechanisms of Chinese medicine hemostatic powder combined with hemostasis after oral administration of Taoren Chengqi Decoction on mice with upper gastrointestinal bleeding. Methods: 24 cases of C57 wild-type male mice were randomly divided into three groups-model group, hemostatic group, and combined group. The three groups established a stress gastrointestinal bleeding model. At two weeks after modeling, the combined group were treated with traditional Chinese medicine hemostatic powder combined with oral Taoren Chengqi Decoction, the hemostatic group were treated with traditional Chinese medicine hemostatic powder, and the model group were treated with the same dose of normal saline. Results: All mice were survived during the experiment. The body weight of the hemostatic group and the combined group were higher than the model group in the 2nd and 4th weeks of treatment (P<0.05), and the combined group were higher than the hemostatic group (P<0.05). The expression levels of serum interleukin (IL)-6 and IL-8 in the hemostasis group and the combination group were lower than the model group in the 2nd and 4th weeks of treatment(P<0.05) , and the combined group were lower than the hemostatic group (P<0.05). The bleeding index of peptic ulcer in the hemostatic group and the combined group were lower than the model group in the 2nd week and the 4th week of treatment(P<0.05), and the combined group were lower than the hemostatic group (P<0.05). The relative expression levels of nuclear factor kappa-B (NF-κB) and Caspase-3 protein in gastric epithelial tissues in the hemostasis group and the combined group were lower than the model group in the 2nd week and the 4th week of treatment(P<0.05), The combined group were lower than the hemostatic group (P<0.05). Conclusion: The application of Chinese medicine hemostatic powder combined with hemostasis after oral administration of Taoren Chengqi Decoction in mice with stress gastrointestinal bleeding can reduce gastrointestinal tissue damage, inhibit the expression of inflammatory factors, and promote the normal development of mice. Its mechanism action may be related to inhibit NF-κB and Caspase-3 protein expression. |
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