| 王青瑛,王郁金,邢文文,闫 颖,王相东.苦杏仁苷介导人肝癌细胞HuH-7凋亡的实验研究[J].现代生物医学进展英文版,2021,(17):3227-3231. |
| 苦杏仁苷介导人肝癌细胞HuH-7凋亡的实验研究 |
| Experimental Study on Amygdalin Mediated Apoptosis of Human Hepatocellular Carcinoma Cell Line Huh-7 |
| Received:December 27, 2020 Revised:January 23, 2021 |
| DOI：10.13241/j.cnki.pmb.2021.17.006 |
| 中文关键词: 苦杏仁苷 HuH-7细胞 凋亡 异体移植 |
| 英文关键词: Amygdalin HuH-7 cells Apoptosis Xenograft |
| 基金项目:陕西省中医管理局中医药科研项目（JCPT005) |
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| 中文摘要: |
| 摘要 目的：通过体内体外实验探讨苦杏仁苷在肝癌中的抗肿瘤作用。方法：MTT 法检测不同浓度的苦杏仁苷对肝癌 HuH-7细胞存活率的影响；DAPI染色法观察苦杏仁苷介导HuH-7细胞的凋亡形态学变化；流式细胞术检测苦杏仁苷干预后HuH-7细胞凋亡率变化；Western Blot法检测细胞凋亡相关蛋白Bax、Bcl-2的表达，计算Bax/Bcl-2的比值。建立裸鼠HuH-7细胞移植瘤模型，观察苦杏仁苷对荷瘤裸鼠移植瘤体积的影响。结果：体外实验结果证实苦杏仁苷能够诱导人肝癌HuH-7细胞凋亡的发生（P<0.05）。随着苦杏仁苷浓度的增加，HuH-7细胞的存活率降低，凋亡率升高，干预后Bax/Bcl-2比值明显升高（P<0.05）。体内实验结果表明，裸鼠HuH-7细胞移植瘤的体积增长速度减慢（P<0.05）。结论：苦杏仁苷能够诱导人肝癌HuH-7细胞和裸鼠HuH-7细胞移植瘤细胞发生凋亡，减缓肿瘤生长，从而发挥抗肿瘤作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the antitumor effect of Amygdalin in liver cancer through vitro and vivo experiments. Methods: MTT assay was used to detect different concentrations of amygdalin on the survival rate of HuH-7 cells; DAPI staining was used to observe the morphological changes of apoptosis induced by amygdalin in HuH-7 cells; flow cytometry was used to detect changes of apoptosis rate of HuH-7 cells after amygdalin intervention; expressions of apoptosis related proteins Bax and Bcl-2 were detected by Western blot, the ratio of Bax / Bcl-2 was calculated. Xenograft tumor model of HuH-7 cells in nude mice was established, and the effect of amygdalin on tumor volume in nude mice was observed. Results: The results of in vitro experiment confirmed that amygdalin could induce apoptosis of human hepatoma cell line HuH-7(P<0.05), with the concentration of amygdalin increased, the survival rate of HuH-7 cells decreased, the apoptosis rate increased, and the ratio of Bax/Bcl-2 increased significantly after intervention(P<0.05). The results of in vivo experiment showed that the growth rate of transplanted tumor of Huh-7 cells in nude mice slowed down(P<0.05). Conclusion: Amygdalin can induce apoptosis of HuH-7 cells and transplanted tumor cells of nude mice HuH-7 cells, slow down tumor growth, and play an anti-tumor effect. |
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