| 张万平,秦 艳,张海鹰,张 鹏,马世军.七氟醚对脑缺血再灌注损伤大鼠认知功能及海马S100β及PGRN表达的影响[J].现代生物医学进展英文版,2021,(17):3222-3226. |
| 七氟醚对脑缺血再灌注损伤大鼠认知功能及海马S100β及PGRN表达的影响 |
| Effects of Sevoflurane on Cognitive Function in Rats with Cerebral Ischemia Reperfusion Injury and the Expression of the S100β and PGRN in Hippocampus |
| Received:February 17, 2021 Revised:March 13, 2021 |
| DOI：10.13241/j.cnki.pmb.2021.17.005 |
| 中文关键词: 脑缺血再灌注损伤 七氟醚 认知功能 S100β PGRN |
| 英文关键词: Cerebral ischemia reperfusion injury Sevoflurane Cognitive function S100β PGRN |
| 基金项目:国家自然科学基金项目(81273902) |
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| 中文摘要: |
| 摘要 目的：探索用七氟醚预处理后脑缺血再灌注损伤大鼠的认知功能变化情况以及海马细胞内钙结合蛋白（S100β）及颗粒蛋白前体（Progranulin,，PGRN）的表达水平。方法：选取SPF级雄性大鼠36只，按照随机数字表法分为假手术组（A组）、脑缺血再灌注损伤组（B组）、七氟醚预处理组（C组），每组12只。B组和C组大鼠采用线栓法建立脑缺血再灌注损伤模型，缺血2 h，再灌注24 h，假手术组仅切开不插入线栓。术前七氟醚预处理组大鼠吸入体积分数3 %七氟醚和氧流量为2 L/min的混合气体，持续1 h，假手术组和脑缺血再灌注损伤组单纯吸入2 L /min的氧气。建模完成后采用大鼠神经功能缺损评分（modified neurological severity score，mNSS）评估大鼠的神经功能状况；Morris水迷宫实验检测各组大鼠学习认知功能；Western Blot检测各组大鼠S100β和PGRN的表达情况。结果：（1）B、C组大鼠mNSS评分显著高于A组，C组评分较B组有明显降低（P＜0.05）；（2）与A组相比B、C两组逃逸潜伏期明显延长，C组与B组相比逃逸潜伏期显著缩短（P＜0.05）；（3）B、C组大鼠海马S100β和PGRN的表达较A组有显著上调，其中C组S100β表达较B组显著降低，PGRN表达显著升高（P＜0.05）。结论：本文通过观察七氟醚预处理对CIRI大鼠认知功能及海马S100β和PGRN蛋白表达水平的影响，发现七氟醚预处理对CIRI大鼠认知功能有显著提高，其作用机制与海马S100β和PGRN的表达密切相关，为进一步探索其作用机制提供参考证据。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the changes of cognitive function and the expression levels of calcium-binding protein (S100β) and granule-protein precursor (PGRN) in hippocampal cells of rats pretreated with sevoflurane after cerebral ischemia reperfusion injury. Methods: 36 SPF male rats were selected and divided into sham operation group (group A), cerebral ischemia reperfusion injury group (group B) and sevoflurane pretreatment group (group C) according to random number table method, with 12 rats in each group,Cerebral ischemia/reperfusion injury models were established in group B and group C by wire embolization method. The rats were treated with ischemia for 2h and reperfusion for 24h. The sham operation group was only cut without inserting wire embolization. Rats in the sevoflurane preconditioning group were inhaled a mixture of sevoflurane with a volume fraction of 3% and an oxygen flow of 2 L /min for 1h, while the sham operation group and the cerebral ischemia/reperfusion injury group were simply inhaled oxygen of 2 L/min.After the modeling, the neurological function of the rats was evaluated by the Rat Neurological Deficiency Score (MNSS). Morris water maze test was used to detect the learning and cognitive function of rats in each group;The expression of S100βand pGRN in each group was detected by Western Blot. Results: （1）Mnss score in groups B and C was significantly higher than that in group A, and score in group C was significantly lower than that in group B(P<0.05).（2）Compared with group A, the escape latency of group B and group C was significantly longer, and the escape latency of group C was significantly shorter than that of group B(P<0.05). （3）Compared with group A, the expression of S100β and pGRN in the hippocampus of rats in groups B and C were significantly up-regulated, and the expression of S100β in group C was significantly decreased compared with group B, while the expression of pGRN was significantly increased (P<0.05). Conclusion: In this study, the effects of sevoflurane pretreatment on cognitive function and the protein expression levels of S100β and PGRN in hippocampus of CIRI rats were observed. The results show that sevoflurane pretreatment significantly improved the cognitive function of CIRI rats, and the mechanism of action was closely related to the expression of S100β and PGRN in hippocampus, which provided evidence for further exploration of the mechanism of action. |
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