| 任 娟,都 燕,金蕾蕾,黑江荣,张 锦,任 东,芦 笛.妊娠期母体甲状腺功能减退对胎鼠骨骼肌胰岛素抵抗和线粒体功能的影响[J].现代生物医学进展英文版,2021,(15):2834-2837. |
| 妊娠期母体甲状腺功能减退对胎鼠骨骼肌胰岛素抵抗和线粒体功能的影响 |
| Effects of Maternal Hypothyroidism during Pregnancy on Insulin Resistance and Mitochondrial Function in Skeletal Muscle of Fetal Rats |
| Received:March 03, 2020 Revised:March 29, 2020 |
| DOI：10.13241/j.cnki.pmb.2021.15.007 |
| 中文关键词: 妊娠期母体甲状腺功能减退 胎鼠 骨骼肌胰岛素抵抗 线粒体功能 |
| 英文关键词: Maternal hypothyroidism during pregnancy Fetal rats Skeletal muscle insulin resistance Mitochondrial function |
| 基金项目:国家自然科学基金项目（41761100） |
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| 中文摘要: |
| 摘要 目的：研究妊娠期母体甲状腺功能减退对胎鼠骨骼肌胰岛素抵抗和线粒体功能的影响。方法：构建妊娠期母体甲状腺功能减退小鼠模型，制备胎鼠骨骼肌线粒体，同时选取健康正常胎鼠做本次实验的对照组。采用酶联免疫吸附法和放射免疫分析法测定两组小鼠甲状腺功能；通过全自动生化分析仪检测两组胎鼠胰岛素抵抗结果；利用Clark氧电极测定密闭反应体系评价两组胎鼠线粒体功能结果，并分析母体甲状腺功能减退与胎鼠骨骼肌胰岛素抵抗和线粒体功能的相关性。结果：两组小鼠甲状腺功能结果、两组胎鼠骨骼肌胰岛素抵抗和线粒体结果对比分析之间均有显著差异（P<0.05）。甲减组小鼠血清中促甲状腺激素（Thyroid Stimulating Hormone，TSH）、丙氨酸转氨酶（alanine aminotransferase，ALT）和门冬氨酸氨基转移酶（aspartate aminotransferase，AST）均较对照组低，胎鼠的空腹血糖（fasting blood-glucose，FBG）、动态3（state3，ST3）、动态4（state4，ST4）呼吸速率和呼吸控制比（respiratory control，RCR）也均较对照组低；而甲减组小鼠游离甲状腺素（free thyroxine，FT4）却较对照组高，胎鼠的胰岛素（insulin，INS）和胰岛素抵抗结果（homeostasis model assessment of insulin resistance，HOMA-IR）也较对照组高（P<0.05）。且母体甲状腺功能减退指标中FT4和RCR、ALT和FBG以及RCR之间有负相关关系，母体甲状腺功能减退的其他指标则与胎鼠骨骼肌胰岛素抵抗和线粒体功能的其他相关指标之间呈正相关关系（P<0.05）。结论：妊娠期母体甲状腺功能减退会降低胎鼠骨骼肌胰岛素抵抗和线粒体功能，影响胎鼠的正常发育。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of maternal hypothyroidism during pregnancy on insulin resistance and mitochondrial function in skeletal muscle of fetal rats. Methods: A mouse model of maternal hypothyroidism during pregnancy was established to prepare skeletal muscle mitochondria of fetal mice, and healthy normal fetal mice were selected as the control group. The thyroid function of the two groups of mice was determined by elisa and radioimmunoassay. The results of insulin resistance in two groups of fetal mice were detected by automatic biochemical analyzer. Clark oxygen electrode was used to measure the closed reaction system to evaluate the mitochondrial function of the two groups of fetal mice, and the correlation between maternal hypothyroidism and insulin resistance in skeletal muscle and mitochondrial function was analyzed. Results: The results of thyroid function in the two groups of mice, the results of thyroid function in the two groups of mice and the results of mitochondria in the two groups of fetal mice were significantly different from each other (P<0.05), which was statistically significant. The mice in the hypothyroidism group had lower TSH, ALT and AST than those in the control group, and the respiratory rate and RCR of FBG, ST3 and ST4 in the fetal mice were also lower than those in the control group. However, the mice in the hypothyroidism group had higher FT4, and the fetal mice had higher INS and HOMA-IR（P<0.05）. In addition, there was a negative correlation between FT4, RCR, ALT, FBG and RCR in maternal hypothyroidism, and a positive correlation between other maternal hypothyroidism and insulin resistance in skeletal muscle and other related indicators of mitochondrial function in fetal rats（P<0.05）. Conclusion: Maternal hypothyroidism during pregnancy can reduce insulin resistance in skeletal muscle and mitochondrial function in fetal rats, and affect the normal development of fetal rats. |
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