| 陈艳霞,哈尼克孜·吐尔逊,梁凌云,刘新莲,马彩玲.长链非编码RNA SNHG14/miR-211对宫颈癌细胞的增殖、侵袭能力的影响[J].现代生物医学进展英文版,2021,(14):2622-2625. |
| 长链非编码RNA SNHG14/miR-211对宫颈癌细胞的增殖、侵袭能力的影响 |
| Effects of Long Non-coding RNA SNHG14/miR-211 on the Proliferation and Invasion of Cervical Cancer Cells |
| Received:February 01, 2021 Revised:February 23, 2021 |
| DOI：10.13241/j.cnki.pmb.2021.14.005 |
| 中文关键词: miR-211 长链非编码RNA SNHG14 宫颈癌 细胞增殖 细胞侵袭 黏蛋白4 |
| 英文关键词: MiR-211 Long non-coding RNA SNHG14 Cervical cancer Cell proliferation Cell invasion Mucin 4 |
| 基金项目:新疆维吾尔自治区自然科学基金面上项目（2020D01C238）；省部共建中亚高发病成因与防治国家重点实验室开放课题项目（SKL-HIDCA-2020-16；SKL-HIDCA-2019-5） |
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| 中文摘要: |
| 摘要 目的：探讨长链非编码(Long chain non-coding，Lnc)RNA SNHG14/微小RNA(microRNA，miR)-211对宫颈癌细胞的增殖、侵袭能力的影响。方法：宫颈癌细胞株SiHa设三组：空白组(不进行转染)、对照组(转染miR-NC)与miR-211组(转染miR-211 mimic)， 噻唑蓝[3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide，MTT] 检测细胞增殖活性，Transwell检测细胞侵袭及转移，实时荧光定量核酸扩增检测系统(Real-time Quantitative PCR, qPCR)检测LncRNA SNHG14与miR-211mRNA水平，Westem印迹法检测黏蛋白4(mucin 4, MUC4)蛋白水平。结果：转染后24 h与48 h，miR-211组的细胞增殖、侵袭、转移指数与LncRNA SNHG14与MUC4蛋白相对表达水平低于空白组和对照组(P<0.05)，miR-211 mRNA表达水平高于空白组(P<0.05)，空白组与对照组对比差异无统计学意义(P>0.05)。结论：过表达miR-211可抑制LncRNA SNHG14的表达，也能抑制MUC4表达，从而能抑制宫颈癌细胞的增殖、侵袭及转移。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of long chain non-coding (Lnc) RNA SNHG14/microRNA(miR)-211 on the proliferation and invasion of cervical cancer cells. Methods: Cervical cancer cell line SiHa were divided into three groups: blank group (without transfection), control group (transfected with miR-NC) and miR-211 group (transfected with miR-211 mimic), used the MTT to detect cell proliferation activity, Transwell to detect the cell invasion and metastasis, qPCR to detect of LncRNA SNHG14 and miR-211 mRNA levels, Western blotting to detect of of Mucin 4(MUC4) protein levels. Results: At 24 h and 48 h after transfection, the cell proliferation, invasion, metastasis index and the relative expression levels of LncRNA SNHG14 and MUC4 in the miR-211 group were lower than those in the blank group and the control group (P<0.05), and the expression of miR-211 mRNA The level were higher than that of the blank group (P<0.05), and the difference compared between the blank group and the control group were not statistically significant (P>0.05). Conclusion: Over-expression of miR-211 can inhibit the expression of LncRNA SNHG14, which can inhibit the expression of MUC4, thereby inhibit the proliferation, invasion and metastasis of cervical cancer cells. |
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