| 孟巧云,王 雪,邱国萍,张 苗,戴 春.雌激素对肾缺血再灌注损伤大鼠Th17/Treg平衡、氧化应激及肾组织NF-κB、TGF-β1表达的影响[J].现代生物医学进展英文版,2021,(10):1844-1848. |
| 雌激素对肾缺血再灌注损伤大鼠Th17/Treg平衡、氧化应激及肾组织NF-κB、TGF-β1表达的影响 |
| Effects of Estrogen on Th17/Treg Balance, Oxidative Stress and Expression of NF-κB and TGF-β1 in Renal Tissue of Rats with Renal Ischemia-reperfusion Injury |
| Received:January 02, 2021 Revised:January 27, 2021 |
| DOI：10.13241/j.cnki.pmb.2021.10.009 |
| 中文关键词: 肾缺血再灌注损伤 大鼠 雌激素 氧化应激 Th17/Treg平衡 |
| 英文关键词: Renal ischemia-reperfusion injury Rat Estrogen Oxidative stress Th17/Treg balance |
| 基金项目:江苏省自然科学基金项目（BK20151152） |
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| 中文摘要: |
| 摘要 目的：研究雌激素对肾缺血再灌注损伤（RIRI）大鼠Th17/Treg平衡、氧化应激及肾组织NF-κB、TGF-β1表达的影响。方法：取60只SD大鼠进行实验，随机分作假手术组、模型组以及雌激素组，每组各20只。模型组及雌激素组均建立RIRI模型，假手术组仅暴露肾脏，不进行肾缺血处理。雌激素组大鼠予以雌激素干预，模型组和假手术组均予以适量的生理盐水干预。分析各组肾功能指标水平以及Paller肾小管损伤评分，Th17/Treg平衡指标水平，氧化应激及肾组织NF-κB、TGF-β1表达水平的差异。结果：模型组及雌激素组血肌酐（SCr）、尿素氮（BUN）水平以及Paller肾小管损伤评分均高于假手术组，且雌激素组上述各项指标水平均低于模型组（均P＜0.05）。模型组及雌激素组白细胞介素-17（IL-17）水平高于假手术组，且雌激素组低于模型组；模型组及雌激素组白细胞介素-10（IL-10）水平低于假手术组，且雌激素组高于模型组（均P＜0.05）。模型组及雌激素组丙二醛（MDA）水平均高于假手术组，且雌激素组低于模型组；模型组及雌激素组超氧化物歧化酶（SOD）水平均低于假手术组，且雌激素组高于模型组（均P＜0.05）。模型组及雌激素组肾组织NF-κB、TGF-β1表达水平均高于假手术组，且雌激素组低于模型组（均P＜0.05）。结论：雌激素可有效调节RIRI大鼠Th17/Treg平衡，减轻氧化应激反应，同时有利于改善肾组织NF-κB、TGF-β1表达，缓解其RIRI。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effects of estrogen on Th17/Treg balance, oxidative stress and the expression of NF-κB and TGF-β1 in renal tissue of rats with renal ischemia-reperfusion injury (RIRI). Methods: 60 SD rats were selected for the experiment, they were randomly divided into sham operation group, model group and estrogen group, with 20 rats in each group. RIRI models were established in both the model group and the estrogen group, while only the kidney was exposed without renal ischemia in the sham operation group. The rats in the estrogen group were treated with estrogen intervention, and the model group and sham operation group were treated with normal saline intervention. The differences of renal function indexes levels, Paller renal tubular injury score, Th17/Treg balance index level, oxidative stress and the expression level of NF-κB and TGF-β1 in renal tissues in each group were analyzed. Results: The levels of serum creatinine (SCr), urea nitrogen (BUN) and Paller renal tubular injury score in model group and estrogen group were higher than those in sham group, and the levels of above indexes in estrogen group were lower than those in model group (all P<0.05). The level of interleukin-17(IL-17) in model group and estrogen group were higher than that in sham operation group, and the estrogen group was lower than the model group. The level of interleukin-10 (IL-10) in model group and estrogen group were lower than that in sham operation group, and the estrogen group was higher than the model group (all P<0.05). The levels of malondialdehyde (MDA) in model group and estrogen group were higher than those in sham operation group, and estrogen group was lower than model group. The level of superoxide gasification enzyme (SOD) in model group and estrogen group was lower than that in sham operation group, and the estrogen group was higher than the model group (all P<0.05). The expression levels of NF-κB and TGF-β1 in renal tissues in model group and estrogen group were higher than those in sham operation group, and the estrogen group was lower than the model group (all P<0.05). Conclusion: Estrogen can effectively regulate Th17/Treg balance in RIRI rats, reduce oxidative stress response, and improve the expression of NF-κB and TGF-β1 in renal tissue, and alleviate RIRI. |
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