| 黄光斌,徐炎安,王 攀,李 涛,胡 平,彭海涛.国产多孔钽材料修复兔胫骨缺损的实验研究[J].现代生物医学进展英文版,2021,(10):1839-1843. |
| 国产多孔钽材料修复兔胫骨缺损的实验研究 |
| Experimental Study on the Repair of Rabbit Tibia Defect with Porous Tantalum Materia |
| Received:August 30, 2020 Revised:September 23, 2020 |
| DOI：10.13241/j.cnki.pmb.2021.10.008 |
| 中文关键词: 多孔钽 胫骨缺损 组织修复 骨整合 |
| 英文关键词: Porous tantalum Defect of tibia Tissue repair Bone integration |
| 基金项目:重庆市卫计委医学科研项目（2016HBRC007）；重庆市科委社会事业与民生保障科技创新专项（cstc2016shms-ztzx10001） |
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| 中文摘要: |
| 摘要 目的：研究国产多孔钽材料能否在兔胫骨缺损模型中顺利实现骨长入，用于修复胫骨缺损。方法：在36只新西兰大白兔双侧胫骨骨干处建立骨缺损模型，每只动物左右侧缺损随机分组，分别进入实验组(植入多孔坦材料)和对照组（不植入多孔坦材料）。植入后4周、8周和12周取材，通过X线检测以及硬组织切片苏木精伊红染色，检测多孔钽材料与骨界面的骨整合情况。采用推出实验检测多孔钽材料与骨界面的结合强度。结果：将术后不同时间点取得的胫骨标本作X射线拍片分析，4周时，骨缺损端与材料结合部位有骨质生成，在8周时材料表面有骨形成现象，逐渐完全覆盖材料表面，在12周时骨量继续增加，形成覆盖材料并桥接骨缺损断端的骨痂。样本行硬组织切片并行HE染色后检测，植入4周后实验组材料两端被新生骨所覆盖，材料深部的孔隙中也可见少量骨组织长入；植入8周后发现实验组材料与骨组织生长良好，多孔钽材料表面和两端材料孔隙内均有骨组织长入，材料孔隙与组织紧密连接，有骨小梁长入；植入12周时两端骨组织长入深度没有明显变化，但材料表面骨组织继续长入，并完全嵌入圆柱体材料内。材料植入后4周与8周比较差异无统计学意义（P＞0.05），材料植入后8周与12周比较差异有统计学意义（P<0.05）。将植入4周、8周和12周后含材料样本置于动态疲劳试验机上进行推出实验，随时间延长所需推出力明显增加，植入后4周和8周相比，虽然后者所需推力较大，但两者比较差异无统计学意义（P＞0.05），而8周和12周比较则差异有统计学意义（P<0.05）。结论：国产多孔坦材料能在胫骨缺损中实现与骨整合，能用于皮质骨缺损修复。 |
| 英文摘要: |
| ABSTRACT Objective: To study whether the domestic porous tantalum can successfully grow with the end of cortical bone and repair the bone defect in the rabbit tibia defect model. Methods: A bone defect model was established at the bilateral tibial diaphysis in 36 New Zealand rabbits. Defects on the left and right sides of each animal were randomly assigned to the experimental group (porous tantalum implanted) and the control group(no tantalum implanted). At 4, 8 and 12 weeks after implantation, the bone integration at the interface between porous tantalum and bone was detected by X-ray examination and hematoxylin eosin staining of hard tissue sections. The bonding strength between porous tantalum and bone was measured by pusher experiment. Results: The tibial specimens of different time points obtained X-ray film analysis. Four weeks, bone defect and material combined with a few bone. Bone formation phenomenon of material surface observed eight weeks, which completely cover material surface and gradually increase bone mass in 12 weeks. At last the callus formation cover material and bridge the whole bone defects. After 4 weeks of implantation, both ends of the materials of the experimental group were covered by new bone, and a small amount of bone tissue was also observed in the deep pores of the materials. After 8 weeks of implantation, it was found that the material and bone tissue of the experimental group grew well. Bone tissue grew on the surface of porous tantalum material and in the pores of both ends of the material. After 12 weeks of implantation, there was no significant change in the depth of bone tissue growth at both ends, but bone tissue continued to grow on the surface of the material and was completely embedded in the cylindrical porous tantalum. There was no statistically significant difference between 4 weeks and 8 weeks after material implantation(P>0.05), while there was a statistically significant difference between 8 weeks and 12 weeks after material implantation (P<0.05). The material be implanted in 4 weeks, 8 weeks and 12 weeks and the samples receive mechanical analyses. With the extension of time, the force of rolling out increased significantly. Compared with the result of 4 weeks and 8 weeks after implantation, the latter required bigger thrust. But the difference is no statistical (P>0.05). While differences of 8 and 12 weeks is statistically significant (P<0.05). Conclusion: Domestic porous tan can achieve osseointegration in rabbit tibia defect and can be used in cortical bone defect repair. |
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