| 毛应岚,刘克舜,姜 维,胡 娜,于娇妍,张 琰,郑 瑾.川芎白芷对过敏性皮炎模型的血流变及炎性因子表达影响的实验研究[J].现代生物医学进展英文版,2021,(10):1829-1833. |
| 川芎白芷对过敏性皮炎模型的血流变及炎性因子表达影响的实验研究 |
| Experimental Study on the Effect of Ligusticum Chuanxiong and Angelica on Hemorheology and Expression of Inflammatory Factors in Allergic Dermatitis Model |
| Received:September 21, 2020 Revised:October 16, 2020 |
| DOI：10.13241/j.cnki.pmb.2021.10.006 |
| 中文关键词: 川芎 白芷 过敏性皮炎 血流变 炎症因子 |
| 英文关键词: Ligusticum chuanxiong Angelicae Dahuricae Allergic dermatitis Hemorheology Inflammatory factors |
| 基金项目:陕西省重点研发计划一般项目（2019SF-279）；空军军医大学军事医学"珠峰工程"；陕西省中医药管理局中医药项目（15JC017） |
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| 中文摘要: |
| 摘要 目的：从皮下血流变和炎性因子表达的角度，研究川芎白芷对过敏性皮炎组织的干预作用。方法：将60只雄性纯白豚鼠随机分为正常组、模型组、白芷组、川芎组、川芎白芷合用组和阳性药组。除正常组外，其余各实验组均使用2.4-二硝基氯苯法建立变应性接触性皮下炎症模型。建模成功后每组按设计分别在致炎各处涂抹各组药物，末次给药4 h后使用激光多普勒血流成像系统检测各组豚鼠患侧耳血流变化，取各实验组豚鼠耳组织，制备HE染色的病理切片，并使用酶联免疫吸附试验(ELISA)法检测耳组织中炎症指标因子IL-1β、TNF-α、LTB4、LTD4、PGE2和PGD2的水平。结果：与正常组比，致敏模型组的耳枝干与末端血管血流急剧增加，各给药组与模型组比较耳血管血流变明显降低，其中川芎白芷合用对炎症组织枝干血管血流影响最显著；模型的相关炎性因子水平均显著升高，各给药组炎症因子指标显著下降，LTB4、LTD4、PGD2、PGE2水平下降。川芎组与白芷组相比，抑制白细胞三烯指标LTB4、LTD4的作用更显著，白芷在IL-1β、IL-8作用较明显，川芎白芷合用组的作用优于川芎和白芷单用组。结论：川芎白芷对急性皮下炎症模型有治疗作用，可抑制组织中炎症反应，减少前列腺素类及白三烯类物质的释放，改善炎症引起的血流变反应。川芎白芷对皮下急性炎症的作用机制有差别，在抑制白细胞三烯类和白介素类炎症介质过程中有协同配伍效果。 |
| 英文摘要: |
| ABSTRACT Objective: To study the intervention effect of Angelica Ligusticum chuanxiong on the tissues of allergic dermatitis from the perspective of subcutaneous blood rheology and inflammatory factor expression. Methods: 60 male pure white guinea pigs were randomly divided into control group, model group, Angelica dahurica group, Ligusticum chuanxiong group, Angelica combined Ligusticum chuanxiong group and positive drug group. Except for the normal group, the allergic contact subcutaneous inflammation model was established by 2.4-dinitrochlorobenzene method in all experimental groups except the normal group. After successful modeling, each group was designed to smear each group of drugs at inflammation sites. Four hours after the last administration, the blood flow changes in the affected ear of guinea pigs in each group were detected by laser Doppler flow imaging system. The ear tissues of guinea pigs in each experimental group were taken and pathological sections stained with HE were prepared. The inflammatory indicators IL-1beta, TNF-alpha, LTB4, LTD4, PGE in ear tissues were detected by enzyme-linked immunosorbent assay (ELISA). Results: Compared with the normal group, the blood flow of the ear branch trunk and terminal vessels of the sensitized model group increased sharply, and the blood flow of the ear vessels of each administration group was significantly lower than that of the model group. in which the combination of Ligusticum Chuanxiong and Angelica dahuricae had the most significant effect on the blood flow of inflamed tissue branches and trunks; the level of related inflammatory factors in the model is significantly increased, the inflammatory factor indexes of each administration group are significantly decreased, and the levels of LTB4, LTD4, PGD2, and PGE2 are decreased. Compared with the Angelicae group, the Ligusticum chuanxiong group had more significant effects on inhibiting the leukotriene indicators LTB4 and LTD4, and the Angelicae had more obvious effects on IL-1β and IL-8. The combined use of Ligusticum chuanxiong and Angelicae group had better effects than the chuanxiong and Angelicae single-use group. Conclusion: Ligusticum chuanxiong Angelica has a therapeutic effect on the acute subcutaneous inflammation model. It can inhibit the inflammation in the tissue, reduce the release of prostaglandins and leukotrienes, and improve the hemorheological response caused by inflammation. The mechanism of action of Chuanxiong Angelica on acute subcutaneous inflammation is different, and it has a synergistic effect in inhibiting inflammatory mediators of leukotrienes and interleukins. |
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