| 练新荣,承耀中,董彦鹏,朱倩梅,孙 莉.老龄小鼠术后认知功能障碍和肠道菌群失调的关系[J].现代生物医学进展英文版,2021,(10):1801-1805. |
| 老龄小鼠术后认知功能障碍和肠道菌群失调的关系 |
| Relationship between Postoperative Cognitive Dysfunction and Intestinal Dysbacteriosis in Aged Mice |
| Received:October 28, 2020 Revised:November 23, 2020 |
| DOI：10.13241/j.cnki.pmb.2021.10.001 |
| 中文关键词: 认知功能障碍 肠道菌群失调 16S rDNA测序 |
| 英文关键词: Cognitive dysfunction Intestinal dysbacteriosis 16s rDNA sequencing |
| 基金项目:深圳市医疗卫生三名工程项目（SZSM201812069） |
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| 中文摘要: |
| 摘要 目的：评价肠道菌群失调和老龄小鼠术后认知功能障碍的潜在作用机制和关系。方法：清洁级健康雄性老龄小鼠 C57BL/6J 20只，16-17月龄，体重28-35ｇ，采用随机数字表法分为两组：对照组（A=10只）和手术组（AS=10只）。AS组小鼠行剖腹探查术。术后48小时采集小鼠粪便和血清标本，16S rDNA测序检测粪便肠道细菌丰度变化，Elisa法检测血清的IL-1β、IL-6和TNF-α含量。术后3天行Morris水迷宫实验。结果：与A组比较，AS组小鼠术后逃避潜伏期延长，空间探索试验在目标象限所花的时间减少（P＜0.05）；α多样性与β多样性指数分析两组小鼠粪便肠道菌群的丰富度和多样性发生差异性改变，AS组的肠道菌群结构中门（6种）和属（15种）水平相对丰度的差异较显著（P＜0.05）；两组小鼠血清中IL-1β、IL-6和TNF-α水平表达对比差异无统计学意义（P＞0.05）。结论：肠道菌群失调改变可能与老龄小鼠术后认知功能障碍的发病机制有关。 |
| 英文摘要: |
| ABSTRACT Objective: To evaluate the relationship between postoperative cognitive dysfunction and intestinal flora imbalance in elderly mice. Methods: Twenty pathogen-free healthy male mice C57BL/6J mice, aged 16-17 months, weighing 28-35 g, were divided into 2 groups (n=10 each) by random number table method: control group (group A) and operation group (group AS). Exploratory laparotomy was performed in AS group. Fecal and serum samples were collected 48 hours after the operation. The fecal intestinal bacterial abundance was detected by 16S rDNA sequencing. The serum levels of IL-1, IL-6 and TNF-α levels were detected by ELISA. Morris water maze test was performed at 3 days after surgery. Results: Compared with group A, the escape latency was significantly prolonged, the time of Probe Trains in the target quadrant was reduced (P＜0.05). α diversity and β diversity analysis showed that levels of phylum (6 species) and genus (15 species) in as group were significantly changed (P＜0.05). There was no significant difference in the expression of IL-1β, IL-6 and TNF-α between the two groups (P＞0.05). Conclusion: The intestinal dysbacteriosis may be related to the pathogenesis of postoperative cognitive dysfunction in aged mice. |
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