| 莎其尔,哈 斯,邹艳慧,乌达木,邢戈贝莉.内脂素对老年大鼠脑组织损伤和炎症反应的影响分析[J].现代生物医学进展英文版,2021,(8):1430-1433. |
| 内脂素对老年大鼠脑组织损伤和炎症反应的影响分析 |
| Analysis of the Effect of Visfatin on Brain Damage and Inflammation in Aged Rats |
| Received:October 01, 2020 Revised:October 23, 2020 |
| DOI：10.13241/j.cnki.pmb.2021.08.006 |
| 中文关键词: 内脂素 老年大鼠 脑组织损伤 炎症反应 神经元 |
| 英文关键词: Visfatin Aged rats Brain tissue injury Inflammation Neurons |
| 基金项目:国家科技部重点研发计划项目（2018YFC2002004） |
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| 中文摘要: |
| 摘要 目的：探讨与分析内脂素对老年大鼠脑组织损伤和炎症反应的影响。方法：10个月龄老年健康雄性Sprague-Dawley(SD)大鼠60只随机平分为两组-对照组与内脂素组，内脂素组给予腹腔注射重组人内脂素200 μg/次，对照组均给予等量蒸馏水灌胃，2次/w，连续给药4 w。对比两组给药前、给药第2 w、给药第4 w的逃避潜伏期、血清白介素(Interleukin，IL)-6与C-反应蛋白(C-reactive protein，CRP)含量，及给药第4 w的线粒体超氧化物歧化酶(Superoxide dismutase，SOD)与谷胱甘肽过氧化物酶(Glutathione peroxidase，GSH-Px)活性、海马与皮层区神经元凋亡率。结果：所有大鼠在实验期间均出现典型的绕池壁现象。两组给药前逃避潜伏期、血清IL-6与CRP含量对比差异无统计学意义(P>0.05)，给药后两组第2 w、给药第4 w的逃避潜伏期、血清IL-6与CRP含量均降低(P<0.05)，且内脂素组给药第2 w、给药第4 w的上述指标低于对照组，对比均有统计学意义(P<0.05)；内脂素组给药第4 w的线粒体SOD、GSH-Px活性高于对照组，海马与皮层区神经元凋亡率低于对照组，经对比差异有统计学意义(P<0.05)。结论：内脂素在老年大鼠的应用能缓解脑组织损伤，抑制炎症反应，有利于清除过量的自由基，降低神经元的凋亡率。 |
| 英文摘要: |
| ABSTRACT Objective: To explore and analyze the effect of visfatin on brain damage and inflammation in aged rats. Methods: 60 cases of 10-month-old healthy male Sprague-Dawley (SD) rats were equally randomly divided into two groups-the control group and the visfatin group. The visfatin group were given intraperitoneal injection of recombinant human visfatin 200 μg/time, the control group were given equal amount of distilled water by gavage, 2 times/week for 4 consecutive weeks. The escape latency, serum interleukin (IL)-6 and C-reactive protein (C-reactive protein, CRP) levels before administration, the 2nd week of administration, and the 4 th week of administration were compared between the two groups. 4 weeks of mitochondrial superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, hippocampal and cortical neuronal apoptosis rate. Results: All rats were showed typical phenomenon around the pool wall during the experiment. There was no significant difference in the escape latency, serum IL-6 and CRP content between the two groups before administration (P>0.05). The escape latency, serum IL-6 and serum IL-6 and CRP levels in the 2nd week and 4th week after administration The content of CRP was reduced (P<0.05), and the above indicators of the visfatin group were lower than those of the control group on the second and fourth weeks of administration, and the comparison was statistically significant (P<0.05); the visfatin group Mitochondrial SOD and GSH-Px activities were higher than those in the control group on the 4 th week of administration, and the apoptosis rate of neurons in the hippocampus and cortex was lower than that in the control group. The difference was statistically significant(P<0.05). Conclusion: The application of visfatin in aged rats can alleviate brain tissue damage, inhibit inflammatory response, help to remove excess free radicals, and reduce neuronal apoptosis rate. |
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