| 范子琪,徐 勤,秦金保,黄 群,吴昭瑜.酶标仪法测定氨茶碱血药浓度的研究[J].现代生物医学进展英文版,2021,(7):1234-1238. |
| 酶标仪法测定氨茶碱血药浓度的研究 |
| Study on Microplate Reader Method Analysis of the Aminophylline |
| Received:August 23, 2020 Revised:September 18, 2020 |
| DOI：10.13241/j.cnki.pmb.2021.07.007 |
| 中文关键词: 氨茶碱 酶标仪法 血药浓度 房室模型 |
| 英文关键词: Aminophylline Microplate reader method Blood concentration Compartment model |
| 基金项目:国家自然科学基金项目(81660668) |
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| 中文摘要: |
| 摘要 目的：通过酶标仪法，建立一种研究氨茶碱在新西兰兔体内的药代动力学参数及房室模型的分析方法。方法：新西兰兔以剂量15 mg/kg静脉注射氨茶碱后，应用酶标仪法，测定在274 nm 波长处吸光度值，采用直线相关与回归分析进行数据统计分析氨茶碱在体内的药代动力学参数、回收率实验及房室模型分析。结果：血清茶碱在浓度5～30 μg/mL范围内线性关系良好，回归方程为：A= 0.0013C+0.0074，r2=0.991。各浓度氨茶碱回收率均大于90%，平均回收率为95.83±3.83，RSD均小于15%，回收效果良好。通过氨茶碱体内房室模型拟合得：二室模型拟合显示：由消除相，得外推线浓度线性回归方程：lgC=-0.1271t+1.0562；由分布相，得残数浓度线性回归方程为：lgCr =-0.5829t+1.030，综合得其二室模型的药动学方程为：C=22.000e-1.342t +11.381e-0.292t 、T1/2（α）= 0.516 h、T1/2（β）= 2.369 h。一室模型拟合显示：一室模型药动学方程为：lgC= -0.2131t + 1.315，其药时方程为：C= 20.649e-0.491t 、T1/2= 1.412 h；结论：可以用酶标仪法测定氨茶碱体内药代动力学相关参数，其回收率良好，体内代谢符合二室模型，消除半衰期较长。 |
| 英文摘要: |
| ABSTRACT Objective: A microplate reader method was established to study the pharmacokinetic parameters and compartment model analysis of aminophylline in New Zealand rabbits. Methods: After intravenous injection of aminophylline at a dose of 15 mg/kg in New Zealand rabbits, the absorbance at 274 nm was measured by microplate reader method. The pharmacokinetic parameters, recovery experiment and compartment model analysis of aminophylline in vivo were analyzed by linear correlation and regression analysis. Results: The linear relationship of serum theophylline was good in the range of 5～30 μg/mL. The regression equation is A=0.0013C+0.0074, r2=0.991. The recovery rate of aminophylline for each concentration is more than 90%, the average recovery rate is 95.83 ±3.83, RSD is less than 15% and the recovery effect is good. The two-compartment model fitting showed that the linear regression equation of extrapolation concentration is obtained by eliminating the phase: lgC=-0.1271t+1.0562. From the distribution phase, the linear regression equation of residual concentration is lgCr =-0.5829t+1.030, and the pharmacokinetic equation of two-compartment model is C=22.000e-1.342t +11.381e-0.292t , T1/2（α）=0.516 h, T1/2（β）=2.369 h. The fitting of compartment model in aminophylline showed that the pharmacokinetic equation of one-compartment model is: lgC=-0.2131t+1.315, and the drug-time equation is C= 20.649e-0.491t , T1/2 =1.412 h. Conclusion: The pharmacokinetic parameters of aminophylline in vivo can be determined by microplate reader method. The recovery rate is good. Its metabolism in body matches the double compartment model, and the elimination half-life is longer. |
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