| 王玉静,弥 娜,张文强,谢晓莉,侯云飞,杨巧妮.磷酸肌酸钠对高糖培养的心肌细胞凋亡与IL-6、TNF-α表达的影响[J].现代生物医学进展英文版,2021,(6):1036-1039. |
| 磷酸肌酸钠对高糖培养的心肌细胞凋亡与IL-6、TNF-α表达的影响 |
| Effect of Creatine Phosphate Sodium on Cardiomyocyte Apoptosis and Expression of IL-6 and TNF-α Cultured in High Glucose |
| Received:October 02, 2020 Revised:October 25, 2020 |
| DOI：10.13241/j.cnki.pmb.2021.06.008 |
| 中文关键词: 磷酸肌酸钠 心肌细胞 心衰 白介素-6 肿瘤坏死因子-α |
| 英文关键词: Creatine phosphate sodium Cardiomyocytes Heart failure Interleukin-6 Tumor necrosis factor-α |
| 基金项目:陕西省社会发展领域项目（2020SF-263） |
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| 中文摘要: |
| 摘要 目的：探讨磷酸肌酸钠对高糖培养的心肌细胞凋亡与白介素(Interleukin，IL)-6、肿瘤坏死因子(Tumor necrosis factor，TNF)-α表达的影响。方法：SD大鼠心肌细胞分为三组-正常组、心衰组、磷酸肌酸钠组，心力衰竭组用含血清的高糖DMEM培养基(33 mmol/L葡萄糖)培养；磷酸肌酸钠组用含血清的高糖DMEM培养基(33 mmol/L葡萄糖)和100 μmol/L磷酸肌酸钠培养；正常组用含10 %血清的DMEM培养基(5.5 mmol/L葡萄糖)培养。采用MTT法检测细胞增殖指数，流式细胞术检测细胞凋亡指数，酶联免疫检测上清IL-6、TNF-α含量，Western blot法检测半胱氨酸蛋白酶-3(Caspase-3)、B淋巴细胞瘤-2(B-cell lymphoma-2，Bcl-2)蛋白水平。结果：处理后24 h、48 h，磷酸肌酸钠组心肌细胞增殖指数高于心衰组(P<0.05)，细胞凋亡指数、Caspase-3和Bcl-2蛋白、IL-6、TNF-α含量相对表达量低于心衰组(P<0.05)，与正常组对比差异无统计学意义(P>0.05)。结论：磷酸肌酸钠在大鼠心肌细胞心力衰竭模型中的应用能降低心肌细胞Caspase-3、Bcl-2蛋白表达，抑制细胞凋亡，提高细胞增殖指数。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of creatine phosphate sodium on cardiomyocyte apoptosis and the expression of interleukin (IL)-6 and tumor necrosis factor (TNF)-α in high glucose cultured. Methods: The cardiomyocytes of SD rats were divided into three groups-normal group, heart failure group, and sodium creatine phosphate group. The heart failure group was cultured with serum-containing high glucose DMEM medium (33 mmol/L glucose); creatine phosphate sodium group was cultured with serum-containing high glucose DMEM medium (33 mmol/L glucose) and 100 μmol/L creatine phosphate sodium; the normal group was cultured with 10 % serum-containing DMEM medium (5.5 mmol/L glucose). MTT method was used to detect cell proliferation index; flow cytometry was used to detect cell apoptosis index; enzyme-linked immunosorbent assay was used to detect the content of IL-6 and TNF-α in the supernatant; Western blot method was used to detect Caspase-3, B-cell lymphoma-2 (Bcl-2) protein level. Results: 24 h and 48 h after treatment, the proliferation indexes of myocardial cells in the sodium phosphate creatine group were higher than those in the heart failure group (P<0.05), and the apoptosis index, Caspase-3 and Bcl-2 protein, IL-6, TNF-α The relative expression of α content were lower than those in the heart failure group (P<0.05), and there was no significant difference compared with the normal group (P>0.05). Conclusion: The application of creatine phosphate sodium in rat cardiomyocyte heart failure model can reduce the expression of Caspase-3 and Bcl-2 protein in cardiomyocytes, inhibit cell apoptosis and increase cell proliferation index. |
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