| 于鹏艳,温继梨,范 杰,杨 柳,丁 丽,王 骞,赵 欣.多巴胺联合NE对感染性休克致急性肝损伤大鼠肝功能、炎性因子及NF-κB p65蛋白的影响[J].现代生物医学进展英文版,2021,(6):1019-1022. |
| 多巴胺联合NE对感染性休克致急性肝损伤大鼠肝功能、炎性因子及NF-κB p65蛋白的影响 |
| Effects of Training Dopamine Combined with NE on Rats with Acute Liver Injury Caused by Septic Shock |
| Received:September 28, 2020 Revised:October 23, 2020 |
| DOI：10.13241/j.cnki.pmb.2021.06.004 |
| 中文关键词: 多巴胺 NE 感染性休克 急性肝损伤 肝功能 |
| 英文关键词: Dopamine NE Septic shock Acute liver injury Liver function |
| 基金项目:内蒙古自治区自然科学基金项目（2019LH08013） |
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| 中文摘要: |
| 摘要 目的：本研究通过研究多巴胺(Dopamine，DA)和去甲肾上腺素( Noradrenaline，NE)对感染性休克致急性肝损伤大鼠肝功能、炎性因子及NF-NF-κB p65蛋白的影响，以期为临床治疗提供一定的试验依据。方法：以48只SPF级健康雄性SD大鼠为研究对象，根据随机数字表法分为四组，每组12只，分别为对照组，脂多糖( lipopolysaccharide，LPS)组，NE组，DA+NE组。LPS、NE和DA+NE建立感染性休克模型，NE组静脉输注去甲肾上腺素，DA+NE组在NE组的基础上静脉输注DA。对各组大鼠肝功能、炎性因子和NF-κB p65蛋白水平进行检测。结果：与对照组相比，LPS、NE和DA+NE组血清天冬氨酸转氨酶(aspartate transaminase，AST)和丙氨酸转氨酶（alanine Transaminase，ALT）水平均显著升高(P＜0.05)，与LPS组相比，NE和DA+NE组大鼠血清AST和ALT均有不同程度的降低(P<0.05)，与NE组相比，DA+NE组大鼠血清AST和ALT水平降低更显著(P<0.05)。与对照组相比，LPS、NE和DA+NE组血清白细胞介素6(interleukin-6，IL-6)和肿瘤坏死因子(tumornecrosis factor，TNF-α)水平均显著升高(P＜0.05)，与LPS组相比，NE和DA+NE组大鼠血清IL-6和TNF-α均有不同程度的降低(P<0.05)，与NE组相比，DA+NE组大鼠血清IL-6和TNF-α水平降低更显著(P<0.05)。与对照组相比，LPS、NE和DA+NE组NF-κB p65蛋白表达水平均显著升高(P＜0.05)，与LPS组相比，NE和DA+NE组大鼠NF-κB p65蛋白表达均有不同程度的降低(P<0.05)，与NE组相比，DA+NE组大鼠NF-κB p65蛋白表达水平降低更显著(P<0.05)。结论：多巴胺联合NE对对大鼠感染性休克所导致的急性肝损伤具有良好的保护作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of DA combined with NE on liver function, inflammatory factors and NF-NF-κB p65 protein in rats with acute liver injury caused by septic shock, in order to provide a certain experimental basis for clinical treatment. Methods: A total of 48 SPF-grade healthy male SD rats were chosen as research subjects and were randomly divided into four groups,i.e. the control group (n=12), LPS group (n=12), NE group (n=12), and DA+NE group (n=12). The septic shock models were established in the LPS, NE and DA+NE group. The NE group received intravenous infusion of norepinephrine, and the DA+NE group received intravenous infusion of DA on the basis of the NE group's therapy. The liver function, inflammatory factors and NF-κB p65 protein levels of rats in each group were detected. Results: Compared with the control group, the serum AST and ALT levels of the LPS, NE and DA+NE group were significantly increased(P＜0.05). Compared with the LPS group, the serum AST and ALT levels of the rats in the NE and DA+NE groups were decreased to varying degrees(P<0.05). Compared with the NE group, the serum AST and ALT levels of the DA+NE group decreased more significantly(P<0.05). Compared with the control group, the serum IL-6 and TNF-α levels in the LPS, NE and DA+NE group were significantly increased(P<0.05). Compared with the LPS group, the serum IL-6 and TNF-α in the NE and DA+NE group decreased to varying degrees (P<0.05). Compared with the NE group, the serum IL-6 and TNF-α levels in the DA+NE group decreased more significantly(P<0.05). Compared with the control group, the expression of NF-κB p65 protein in the LPS, NE and DA+NE group was significantly increased(P＜0.05). Compared with the LPS group, the expressions of NF-κB p65 protein in the NE and DA+NE group were reduced to varying degrees(P<0.05). Compared with the NE group, the expression of NF-κB p65 protein in the DA+NE group decreased more significantly(P<0.05). Conclusion: Dopamine combined with NE has a good protective effect on acute liver injury caused by septic shock in rats. |
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