| 祁亚宁,马佑平,李 刚,王利新,刘 辉.酶联免疫法和胶体金法在胃镜检查前检测乙肝表面抗原的结果观察[J].现代生物医学进展英文版,2021,(5):903-906. |
| 酶联免疫法和胶体金法在胃镜检查前检测乙肝表面抗原的结果观察 |
| Observation on Results of ELISA and Colloidal Gold Method in Detection of Hepatitis B Surface Antigen Before Gastroscopy |
| Received:August 06, 2020 Revised:August 31, 2020 |
| DOI：10.13241/j.cnki.pmb.2021.05.021 |
| 中文关键词: 酶联免疫法 胶体金法 胃镜检查 乙肝表面抗原 检测结果 |
| 英文关键词: Enzyme linked immunosorbent assay Colloidal gold method Gastroscopy Hepatitis B surface antigen Detection results |
| 基金项目:宁夏回族自治区科学技术厅基金项目（2018AAC03262） |
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| 中文摘要: |
| 摘要 目的：比较酶联免疫法和胶体金法在胃镜检查前检测乙肝表面抗原的结果，以探讨酶联免疫法和胶体金法在检验与病理科乙肝表面抗原检测中的应用价值。方法：选择2017年1月～2019年12月我院进行胃镜检查的100例上消化道疾病患者，均在胃镜检查前检测乙肝表面抗原，对照组使用胶体金法进行检测，观察组使用酶联免疫法进行检测。比较酶联免疫法和胶体金法在胃镜检查前检测乙肝表面抗原的阳性率、敏感度以及特异度。结果：酶联免疫法和胶体金法在胃镜检查前检测乙肝表面抗原的阳性率分别为35.00 %（35/100）和33.00 %（33/100），两种方法相比较没有明显的差异（P>0.05）；酶联免疫法在胃镜检查前检测乙肝表面抗原的敏感度（97.34 %）与特异度（98.12 %）均明显高于胶体金法（P<0.05）。结论：酶联免疫法和胶体金法在胃镜检查前对乙肝表面抗原均有比较高的阳性检测率，但是酶联免疫法具有更高的敏感度以及特异度，仍然是现今检测乙肝表面抗原的主要方法，胶体金法由于检测方法更加简便和直观、方便携带、结果更加快捷，而且不需要特殊的仪器设备，更加适用于临床上紧急状态下患者的检测以及感染者初筛等情况。 |
| 英文摘要: |
| ABSTRACT Objective: To compare the results of enzyme-linked immunosorbent assay and colloidal gold method in the detection of hepatitis B surface antigen before gastroscopy. Methods: A total of 100 patients with upper gastrointestinal diseases, who underwent gastroscopy in Ningxia General Hospital of Armed Police Force from January 2017 to December 2019, were selected and were detected HBsAg before gastroscopy. The control group was detected by colloidal gold method, and the observation group was detected by ELISA. The positive rate, sensitivity and specificity of ELISA and colloidal gold method in detecting HBsAg before gastroscopy were compared. Results: The positive rates of ELISA and colloidal gold in detecting HBsAg before gastroscopy were 35.00 %（35/100） and 33.00 %（33/100）, respectively. There was no significant difference between the two methods（P>0.05）. The sensitivity（97.34 %） and specificity（98.12 %）of ELISA in detecting HBsAg before gastroscopy were significantly higher than those of colloidal gold method（P<0.05）. Conclusion: Two enzyme linked immunosorbent assay and colloidal gold method have high positive detection rate for HBsAg before gastroscopy. However, ELISA is still the main method to detect HBsAg due to its higher sensitivity and specificity. Colloidal gold method is more simple and intuitive, convenient to carry, faster results, and does not need special equipment, which is more suitable for the detection of patients in clinical emergency and the initial screening of infected persons. |
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