| 马文静,斯依提·阿木提,阿地力江·伊明,刘凤霞,侯鹏程.复合应激型ED大鼠模型促炎细胞因子IL-6、TNF-α和TGF-β1水平的研究[J].现代生物医学进展英文版,2021,(5):825-829. |
| 复合应激型ED大鼠模型促炎细胞因子IL-6、TNF-α和TGF-β1水平的研究 |
| Proinflammatory Cytokines IL-6, TNF-α and TGF-β1 Expression in Rats Model Induced by Compound Stress |
| Received:July 31, 2020 Revised:August 30, 2020 |
| DOI：10.13241/j.cnki.pmb.2021.05.005 |
| 中文关键词: 促炎细胞因子 冷刺激 环境雌激素 勃起功能障碍 纤维化 |
| 英文关键词: Proinflammatory cytokines Cold stress Environmental estrogen Erectile dysfunction Fibrosis |
| 基金项目:新疆维吾尔自治区自然科学基金项目（2017D01C239） |
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| 中文摘要: |
| 摘要 目的：探讨环境雌激素样饲料联合冷刺激诱导的ED大鼠血清与阴茎组织中促炎因子IL-6、TNF-α和TGF-β1的变化及其对ED大鼠阴茎组织纤维化的影响。方法：取100只SD雄性大鼠，20只为正常组（N），其余120只为建模组，以环境雌激素样饲料联合冷刺激予以复合干预，24周后通过APO阴茎勃起实验和交配实验筛选ED大鼠，并从中随机选取20只大鼠为勃起功能障碍组(ED组)，以ELISA和Western blot法分别检测各组大鼠血清和阴茎组织中IL-6、TNF-α和TGF-β1表达水平，Masson染色观察阴茎组织纤维化情况。结果：(1) ED组大鼠血清中IL-6、TNF-α和TGF-β1含量较N组显著升高（P<0.05）；(2)ED组大鼠阴茎组织中IL-6、TNF-α 和 TGF-β1 蛋白相对表达水平较N组显著升高(P<0.05)；（3）ED组大鼠阴茎组织平滑肌/胶原纤维比值显著低于N组(P<0.05)。结论：环境雌激素样饲料联合冷刺激建立的复合应激型ED大鼠阴茎组织发生纤维化是其发生ED的重要组织病理学原因之一，具体机制可能和血清和组织中促炎细胞因子高表达密切相关。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the changes of pro-inflammatory factors IL-6, TNF-α and TGF-β1 in the serum and penile tissue of ED rats induced by environmental estrogen-like diet combined with cold stimulation and their effects on the fibrosis of the penile tissue of ED rats. Methods: 120 male Sprague-Dawley rats were randomly divided into 2 groups: normal control group (N, n= 20) were feeding normal environment and diet, and model group (n = 100) were intervened in damp and cold environment and gave estrogen-like feed to model for 24w to screen ED rats, and then ELISA and Western-blot methods were used to detect the expression levels of IL-6, TNF-α and TGF-β1 in the serum and penile tissues of rats in each group, observe the fibrosis of penile tissues by Masson staining. Results: (1) IL-6, TNF-α and TGF-β1 in ED rats serum were significantly increased compared with N group(P<0.05); (2) The relative expression levels of IL-6, TNF-α and TGF-β1 protein in the penile tissue of the ED group were significantly higher compared with N group (P<0.05); (3) The penile tissue smooth muscle/collagen fiber ratio of the ED group was significantly lower compared with N group(P<0.05). Conclusion: The fibrosis of the penile tissue of compound stress ED rats induced by environmental estrogen-like diet combined with cold stimulation is one of the important histopathological reasons for the occurrence of ED. The specific mechanism may be closely related to the high expression of pro-inflammatory cytokines in serum and tissues. |
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