| 聂雨泽,王 燕,杨 帆,杨 艳,孙 薇.天麻素通过下调Nav1.6通道表达缓解糖尿病神经病理性疼痛的研究[J].现代生物医学进展英文版,2021,(4):629-633. |
| 天麻素通过下调Nav1.6通道表达缓解糖尿病神经病理性疼痛的研究 |
| Gastrodin Alleviated Diabetic Neuropathic Pain via Downregulating the Expression of Nav1.6 in Rats |
| Received:July 23, 2020 Revised:August 18, 2020 |
| DOI：10.13241/j.cnki.pmb.2021.04.006 |
| 中文关键词: 糖尿病神经病理性疼痛 天麻素 Nav1.6通道 背根神经节 |
| 英文关键词: Diabetic neuropathic pain Gastrodin Nav1.6 channel Dorsal root ganglion |
| 基金项目:唐都医院科技创新发展基金项目（2019QYTS005）；陕西省重点研发计划项目（2020SF-164）；唐都医院苗子人才资助计划项目；国家自然科学基金项目（31300919） |
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| 中文摘要: |
| 摘要 目的：探究天麻素对 Ⅱ 型糖尿病神经病理性痛的镇痛作用以及天麻素对背根神经节Nav1.6通道的表达调控作用。方法：将60只雄性SD大鼠随机分为空白对照组、糖尿病组和天麻素处理组(10 mg?kg-1?d-1)。通过高脂饮食喂养4周，低剂量腹腔注射STZ (30 mg?kg-1) 的方法构建 Ⅱ 型糖尿病神经病理性痛大鼠模型，利用痛行为学检测观察各组大鼠的机械刺激足缩反应阈值变化，采用免疫荧光组织化学及Western blot方法观察各组大鼠背根神经节上Nav1.6通道的表达变化。结果：与空白对照组相比，糖尿病模型大鼠出现显著的机械刺激疼痛阈值下降 (P<0.05)，且模型组大鼠背根神经节神经元上的Nav1.6通道表达上调 (P< 0.05)。与糖尿病组相比，连续腹腔注射天麻素 3天、7天、14天后，模型动物的疼痛明显缓解 (P<0.05)，另外天麻素可以翻转背根神经节上Nav1.6通道的高表达 (P<0.05)。结论：天麻素可能通过降低Nav1.6通道的表达来缓解 Ⅱ 型糖尿病神经病理性疼痛，从而为天麻素缓解糖尿病神经病理性疼痛提供新的理论依据。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the inhibitory effect of gastrodin on allodynia in diabetic neuropathic pain and its mechanisms. Methods: 144 male SD rats were randomly divided into three groups: control group, diabetes group and gastrodin treatment group (10 mg?kg-1?d-1). Type Ⅱ Diabetic rats were fed high fat diet for 4 weeks and intraperitoneally injected with low-dose streptozotocin (STZ) (30 mg?kg-1). The paw withdrawal mechanical threshold (PWMT) was detected to reflect the pain behavioral changes. The expression of Nav1.6 was observed by immunofluorescence histochemistry and Western blot analysis. Results: Compared with control rats, type Ⅱ diabetic rats showed significant pain sensitization and lower PWMT (P<0.05). Upregulated expression of Nav1.6 channel was detected on dorsal root ganglion (DRG) neurons from these painful diabetic rats (P<0.05). After continuous injection of gastrodin for 3 days, 7 days and 14 days, the allodynia of diabetic rats was significantly relieved (P<0.05). Moreover, gastrodin could reverse the overexpression of Nav1.6 on dorsal root ganglion neurons (P<0.05). Conclusion: Gastrodin could alleviate diabetic neuropathic pain by downregulating the overexpression of Nav1.6. This research may provide new data for clinical therapy in diabetic neuropathic pain. |
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