| 彭金祥,姜卫东,陆晓晨,高 想,郑晓丹,陈爱兰,张锋莉,盛 炜.补肾活血汤联合卡托普利治疗老龄自发性高血压大鼠的机制研究[J].现代生物医学进展英文版,2020,(23):4418-4421. |
| 补肾活血汤联合卡托普利治疗老龄自发性高血压大鼠的机制研究 |
| Mechanism of Bushen Huoxue Decoction Combined with Captopril in the Treatment of Senile Spontaneously Hypertensive Rats |
| Received:May 28, 2020 Revised:June 21, 2020 |
| DOI：10.13241/j.cnki.pmb.2020.23.004 |
| 中文关键词: 补肾活血汤 卡托普利 老龄 自发性高血压 大鼠 炎症因子 血管内皮功能 |
| 英文关键词: Bushen Huoxue Decoction Captopril Senile Spontaneous hypertension Rats Inflammatory factors Vascular endothelial function |
| 基金项目:国家中医药管理局科研专项（JDZX20160489）；江苏省卫生计生委科研项目（H20160342） |
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| 中文摘要: |
| 摘要 目的：探讨补肾活血汤联合卡托普利对老龄自发性高血压大鼠（SHR）的降压疗效并分析其作用机制。方法：选取60只SHR按照随机数字表法分为卡托普利组、补肾活血汤组、联合组、模型组，每组15只，另选取15只Wistar-Kyoto大鼠为空白组。联合组给予补肾活血汤联合卡托普利悬浊液灌胃干预，补肾活血汤组给予补肾活血汤灌胃干预，卡托普利组给予卡托普利悬浊液灌胃干预，模型组和空白组给予蒸馏水灌胃干预，连续干预8周。检测干预前、干预4周后、8周后各组大鼠尾动脉收缩压，对比各组大鼠干预8周后血清炎症因子C反应蛋白（CRP）和肿瘤坏死因子-α（TNF-α）水平以及血清内皮素（ET）、血管紧张素Ⅱ（AngⅡ）、一氧化氮（NO）浓度。结果：干预前后，SHR各组大鼠尾动脉收缩压高于空白组（P<0.05），干预4周后、8周后，联合组、补肾活血汤组、卡托普利组尾动脉收缩压均低于模型组，且联合组尾动脉收缩压低于卡托普利组、补肾活血汤组（P<0.05）；干预后补肾活血汤组与卡托普利组尾动脉收缩压比较差异无统计学意义（P>0.05）。干预8周后，模型组、卡托普利组、补肾活血汤组CRP、TNF-α水平与空白组比较显著升高（P<0.05），联合组CRP、TNF-α水平与空白组比较差异无统计学意义（P>0.05），卡托普利组、补肾活血汤组、联合组CRP、TNF-α水平呈逐渐降低趋势，组间比较差异有统计学意义（P<0.05）。干预8周后，模型组、卡托普利组、补肾活血汤组ET、AngⅡ浓度与空白组比较显著升高（P<0.05），联合组ET、AngⅡ浓度与空白组比较差异无统计学意义（P>0.05），联合组、补肾活血汤组、卡托普利组ET、AngⅡ浓度低于模型组，且联合组低于卡托普利组、补肾活血汤组（P<0.05）；模型组、卡托普利组NO浓度低于空白组、补肾活血汤组、联合组（P<0.05），空白组、补肾活血汤组、联合组NO浓度比较差异无统计学意义（P>0.05）。结论：补肾活血汤联合卡托普利对SHR有明显的降压作用，其机制可能与降低血清炎症因子、ET、AngⅡ含量和升高NO含量有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the antihypertensive effect of Bushen Huoxue Decoction Combined with captopril on senile spontaneously hypertensive rats(SHR) and to analyze its mechanism. Methods: 60 SHR were randomly divided into captopril group,Bushen Huoxue Decoction group, combined group and model group, with 15 rats in each group, 15 Wistar-Kyoto rats were selected as blank group. The combined group was treated with Bushen Huoxue Decoction Combined with captopril suspension, Bushen Huoxue Decoction group given Bushen Huoxue Decoction gavage intervention, the captopril group was given captopril suspension for gastric intervention, Model group and blank group were treated with distilled water for intragastric intervention, continuous intervention for 8 weeks. The systolic blood pressure of the tail arteries of the rats was measured before, 4 weeks and 8 weeks after intervention. The levels of serum inflammatory factors C reactive protein (CRP) and tumor necrosis factor -α (TNF-α), serum endothelin (ET), angiotensin II (Ang II) and nitrogen oxide (NO) were compared for 8 weeks after intervention. Results: Before and after the intervention, the systolic blood pressure of tail artery in each SHR group was higher than that in blank group (P<0.05). After 4 weeks and 8 weeks of intervention, the systolic blood pressure of tail artery in combined group, Bushen Huoxue Decoction group and captopril group was lower than that in model group, and the systolic blood pressure of tail artery in combined group was lower than that in captopril group and Bushen Huoxue Decoction group (P<0.05); after the intervention, the systolic blood pressure of tail artery in was no significant difference between Bushen Huoxue Decoction group and captopril group (P>0.05). After 8 weeks of intervention, the levels of CRP and TNF-α in the model group, captopril group and Bushen Huoxue Decoction group were significantly higher than those in the blank group (P<0.05), there was no significant difference in the levels of CRP and TNF-α between the combined group and the blank group (P>0.05), and the levels of CRP and TNF-α in the captopril group, Bushen Huoxue Decoction group, combined group decreased gradually, and there was significant difference between the groups (P<0.05). After 8 weeks of intervention, the concentrations of ET and AngⅡ in the model group, captopril group and Bushen Huoxue Decoction group were significantly higher than those in the blank group (P<0.05), there was no significant difference in the concentrations of ET and AngⅡ between the combined group and the blank group (P>0.05). The concentrations of ET and AngⅡ in the combined group, Bushen Huoxue Decoction group and captopril group were lower than those in the model group, and the combined group was lower than those in the captopril group and Bushen Huoxue Decoction group (P<0.05). The concentrations of NO in model group and captopril group was lower than that in blank group, Bushen Huoxue Decoction group and combination group (P<0.05), and there was no significant difference in NO concentration among blank group, Bushen Huoxue Decoction group and combination group (P>0.05). Conclusion: Bushen Huoxue Decoction Combined with captopril has obvious antihypertensive effect on SHR, and its mechanism may be related to the decrease of serum inflammatory factors, ET, Ang II content and increasing NO content. |
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