徐蕾蕾,华 馥,李长华,周树林,徐 童.上皮性卵巢癌组织miR-338-3p、miR-1294的表达及临床意义[J].现代生物医学进展英文版,2020,(22):4293-4297. |
上皮性卵巢癌组织miR-338-3p、miR-1294的表达及临床意义 |
The Expressions and Clinical Significance of miR-338-3p, miR-1294 in Patients with Epithelial Ovarian Cancer |
Received:May 07, 2020 Revised:May 31, 2020 |
DOI:10.13241/j.cnki.pmb.2020.22.020 |
中文关键词: 上皮性卵巢癌 miR-338-3p miR-1294 预后 临床病理参数 |
英文关键词: Epithelial ovarian cancer miR-338-3p miR-1294 Prognosis Clinicopathological characteristics |
基金项目:国家自然科学基金面上项目(81472442) |
|
Hits: 657 |
Download times: 625 |
中文摘要: |
摘要 目的:探讨上皮性卵巢癌组织中微小RNA(miR)-338-3p、miR-1294表达与患者临床病理参数的关系,并分析其表达对上皮性卵巢癌预后的影响。方法:收集2010年6月~2015年6月我院行手术治疗的上皮性卵巢癌患者的石蜡组织样本(癌组织和癌旁正常组织),实时荧光定量PCR(RT-PCR)技术检测组织样本中miR-338-3p、miR-1294的表达情况;分析癌组织中miR-338-3p、miR-1294表达与患者临床病理参数的关系;Kaplan-Meier生存曲线分析miR-338-3p、miR-1294表达与患者预后的关系;Cox回归模型分析上皮性卵巢癌患者的预后影响因素。结果:与癌旁正常组织相比,上皮性卵巢癌组织中miR-338-3p、miR-1294表达均降低(均P<0.05);miR-338-3p表达与组织学分级、淋巴结转移、FIGO分期相关(均P<0.05);miR-1294表达与肿瘤直径、淋巴结转移、FIGO分期相关(均P<0.05)。Kaplan-Meier生存曲线结果显示,miR-338-3p低表达组患者、miR-1294低表达组患者的预后较差(均P<0.05)。Cox回归模型分析结果显示,淋巴结转移、较高FIGO分期、miR-338-3p低表达、miR-1294低表达是上皮性卵巢癌预后的危险因素(均P<0.05)。结论:上皮性卵巢癌组织中miR-338-3p、miR-1294均低表达,且均与较差临床病理参数、预后不良相关;miR-338-3p、miR-1294可能是上皮性卵巢癌患者预后预测的潜在指标。 |
英文摘要: |
ABSTRACT Objective: To explore the relationship between microRNA (miR)-338-3p, miR-1294 expression with the clinicopathological parameters in epithelial ovarian cancer tissues, and to analyze their impact on the prognosis in epithelial ovarian cancer. Methods: Paraffin tissue samples (cancer tissue and normal tissue adjacent to cancer) of epithelial ovarian cancer patients in our hospital from June 2010 to June 2015 were collected, real-time quantitative PCR (RT-PCR) was used to detect the expression of miR-338-3p and miR-1294 in tissue samples. The correlation between the miR-338-3p, miR-1294 expression with the clinicopathological characteristics were analyzed. The Kaplan Meier survival curve was used to analyze the relation of miR-338-3p, miR-1294 expression with the prognosis of patients. The Cox regression model was used to analyze the influence factors of prognosis. Results: Compared to the adjacent normal tissues, the miR-338-3p and miR-1294 expression were both down-regulated in the cancer tissues (all P<0.05). The miR-338-3p expression were related with histological grade, lymph node metastasis and the FIGO stage (all P<0.05). The miR-1294 expression were related with tumor diameter, lymph node metastasis and the FIGO stage (all P<0.05). Kaplan Meier survival curve results showed the prognosis of patients with miR-338-3p low expression and miR-1294 low expression were poorer (all P<0.05). Cox regression model analysis results showed that the lymph node metastasis, higher FIGO staging, miR-338-3p low expression and miR-1294 low expression were the risk factors for the prognosis (all P<0.05). Conclusion: In epithelial ovarian cancer, the miR-338-3p and the miR-1294 are all low expression, which are related to worse clinicopathological parameters and poorer prognosis. The miR-338-3p and miR-1294 may be potential prognostic indicators for patients with epithelial ovarian cancer. |
View Full Text
View/Add Comment Download reader |
Close |