| 白国栋,全建峰,魏 辉,贾 勇,江 静.低氧诱导因子基因沉默对胃癌细胞BGC-823微血管生成和血管内皮生长因子表达的影响[J].现代生物医学进展英文版,2020,(22):4229-4233. |
| 低氧诱导因子基因沉默对胃癌细胞BGC-823微血管生成和血管内皮生长因子表达的影响 |
| Effects of Hypoxia-inducible Factor Gene Silencing on Microangiogenesis and Expression of Vascular Endothelial Growth Factor in Gastric Cancer Cell BGC-823 |
| Received:May 08, 2020 Revised:May 31, 2020 |
| DOI：10.13241/j.cnki.pmb.2020.22.006 |
| 中文关键词: 低氧诱导因子 胃癌 微血管生成 血管内皮生长因子 基因沉默 |
| 英文关键词: Hypoxia-inducible factor Gastric cancer Microangiogenesis Vascular endothelial growth factor Gene silencing |
| 基金项目:陕西省中医管理局中医药科研项目（JCPT011） |
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| 中文摘要: |
| 摘要 目的：探讨低氧诱导因子基因沉默对胃癌细胞BGC-823微血管生成和血管内皮生长因子表达的影响。方法：对数生长期的BGC-823细胞株分为三组-实验组、对照组与空白组，分别转染200 ng/mL shRNA-HIF-1α、200 ng/mL shRNA-NC与等体积的磷酸盐缓冲液。采用CCK法检测细胞增殖，流式细胞仪检测细胞凋亡，Transwell小室实验检测细胞迁移与侵袭，Western blot检测蛋白表达，qRT-PCR检测基因表达。结果：细胞转染后24 h与48 h，实验组的HIF-1α相对表达量、细胞增殖指数、细胞迁移与侵袭指数显著低于空白组和对照组(P＜0.05)，细胞凋亡指数显著高于空白组和对照组(P＜0.05)。细胞转染后48 h，与对照组和空白组相比，实验组的血管内皮生长因子(vascular endothelial growth factor，VEGF) 蛋白相对表达水平显著降低(P＜0.05)。结论：沉默HIF-1α表达能抑制胃癌细胞BGC-823微血管生成和VEGF表达，从而抑制胃癌细胞增殖、转移与侵袭，促进细胞凋亡。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of hypoxia-inducible factor gene silencing on microangiogenesis and expression of vascular endothelial growth factor in gastric cancer cell BGC-823. Methods: BGC-823 cell lines in logarithmic growth phase were divided into three groups-experimental group, control group and blank group, respectively transfected with 200 ng/mL shRNA-HIF-1α, 200 ng/mL shRNA-NC and equal volume phosphate buffer. Cell proliferation were detected by CCK method, cell apoptosis were detected by flow cytometry, cell migration and invasion were detected by Transwell cell experiment, protein expression were detected by Western blot, and gene expression were detected by qRT-PCR. Results: 24 h and 48 h after cell transfection, the relative expression level of HIF-1α, cell proliferation index, cell migration and invasion index in the experimental group were significantly lower than those in the blank group and control group (P<0.05), and the apoptosis index were significantly higher Blank group and control group (P<0.05). At 48 h after cell transfection, compared with the control group and the blank group, the relative expression level of VEGF protein in the experimental group were significantly reduced (P<0.05). Conclusion: Silencing the expression of HIF-1α can inhibit the microangiogenesis and VEGF expression of gastric cancer cell BGC-823, thereby inhibiting the proliferation, metastasis and invasion of gastric cancer cells and promoting apoptosis. |
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