| 胡 娜,姜 维,于娇妍,年 轮,张 琰.白芷冰片对皮下炎症模型的血流变及抗炎作用研究[J].现代生物医学进展英文版,2020,(21):4013-4018. |
| 白芷冰片对皮下炎症模型的血流变及抗炎作用研究 |
| Study on the Effect of Angelica-Borneol on Hemorheology and Anti-inflammatory on Subcutaneous Inflammatory Model |
| Received:April 15, 2020 Revised:May 11, 2020 |
| DOI：10.13241/j.cnki.pmb.2020.21.003 |
| 中文关键词: 白芷 冰片 皮下炎症 血流变 炎症因子 |
| 英文关键词: Angelica Borneol Subcutaneous inflammation Blood flow Inflammatory factor |
| 基金项目:空军军医大学军事医学"珠峰工程"；陕西省重点研发计划一般项目（2019SF-279） |
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| 中文摘要: |
| 摘要 目的：从血流变和抑制炎症因子角度探讨白芷冰片的抗皮肤炎症作用及机制。方法：将60只SD大鼠随机分为 6 组: 正常组、模型组、复方醋酸地塞米松乳膏组、白芷组、冰片组和白芷冰片合用组。除正常组外，其余各实验组均使用角叉菜胶注射足底建立足皮下炎症模型。建模成功1 h后每组按设计分别在致炎处涂抹各组药物，给药4h后使用激光多普勒血流成像系统检测各组大鼠足趾血流变化，并测量足趾肿胀度，建模4 h后取炎症组织，以Elisa法检测肿胀足组织中SOD、HIS、MDA、PGE2、PGD2、COX-2以及IL-6、IL-1β、IL-8、IL-10的水平。结果：与正常组比，炎症模型组的足趾肿胀率、血流变显著增加；各给药组与模型组比较足趾肿胀率明显降低，各给药组对血流变影响差异显著，其中白芷对末梢微循环的作用明显，而冰片对足趾枝干血流变影响更显著，合用后对总体血流变有改善作用；相关细胞因子结果显示，模型组除SOD外，其余HIS、MDA、PGE2、PGD2、COX-2、IL-6、IL-1β、IL-8、IL-10显著性升高，白芷、冰片及合用组与模型组比较，各项指标因子均有不同程度的改善，其中冰片对SOD和HIS作用较弱，白芷与合用组可明显降低PGD2、COX-2、IL-6、IL-1β、IL-8指标( P ＜ 0.05 )；白芷、冰片及合用组对IL-10无明显作用。结论：白芷联合冰片对角叉菜胶诱导的急性炎症有明显的抗炎治疗作用，可显著降低水肿反应，抑制微循环扩张引起的血流变增加，其机制可能与PGE2、COX-2以及多种白介素有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the anti-skin inflammation effect and mechanism of Angelicae and Borneol from the perspective of blood rheology and inhibition of inflammatory factors. Methods: The 60 SD rats were randomly divided into 6 groups:Normal group, Model group, Positive control group, Borneol group and Angelica-Borneol group. In addition to the Normal group, the rest of the group are established using carrageen glue injection of plantar foot skin, Inflammation model modeling success in each group after 1 h in inflammatory place daub each group se-parately according to the design of drugs, dosage 4 h after Laser Doppler blood flow imaging system were used to detect each group rats toes blood flow changes, and to measure toes swelling degree, modeling, inflammation tissue after 4 h with Elisa method to detect swollen foot organiz ation of SOD HIS PGE2, MDA PGD2 COX-2 and IL - 6、IL-1β, IL-8 level. Results: The toe swelling rate of the inflammatory Model group is increased significantly compared to the Normal group. After exposure to different drugs, the rate decreased sharply. And, there are more clear distinctions of the hemorheology between each group, especially the Angelica group which had a dramatic changein the branches of the toes, and the Borneol group had a more significant amelioration on peripheral microcirculation. The combination of two drug improved hemorheology globally .The evaluation of related cytokines showed that, except SOD, the model group showed significant increment of HIS, MDA, PGE2, PGD2, COX-2, Il-6, IL-1β, and IL-8 (P < 0.05). After treatment, Baizhi, Borneol and the combined group eased these indicators in different degrees compared to the model group. Borneol had a weaker effect on SOD and HIS, and Baizhi and the combined group could significantly reduce PGD2, COX-2, IL-6, IL -1β, IL-8 (P＜ 0.05); while they all had no obvious effect on IL-10. Conclusion: Angelica combined with Borneol has obvious anti-inflammatory effect on acute inflammation. Also, this combination group can decrease the side effects of edema and inhibit the increase of hemorheology caused by the expansion of microcirculation. The mechanism may be related to PGE2, COX-2 and A variety of interleukins. |
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