| 连婧阁,郑林丰,段 猛,李茂林,嵇 颖,高 国,李康安.Fe3O4-PEG-CD56/Avastin@Ce6纳米探针的合成及细胞MR成像初步研究[J].现代生物医学进展英文版,2020,(16):3006-3010. |
| Fe3O4-PEG-CD56/Avastin@Ce6纳米探针的合成及细胞MR成像初步研究 |
| Pilot Study of Synthesis and In Vitro MRI of Fe3O4-PEG-CD56/Avastin@Ce6 Nanoprobes |
| Received:January 29, 2020 Revised:February 25, 2020 |
| DOI：10.13241/j.cnki.pmb.2020.16.002 |
| 中文关键词: 分子探针 靶向成像 自然杀伤细胞 |
| 英文关键词: Molecular probe Targeting imaging Natural kill cell |
| 基金项目:国家自然科学基金项目（81972872，11826020）；上海市科委科技创新项目（17441900700）；上海市申康科研项目（16CR3091B）；上海交通大学医工交叉基金项目（YG2015MS31） |
| Author Name | Affiliation | E-mail | | LIAN Jing-ge | Department of Radiology, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai, 200080, China | liankanlian@126.com | | ZHENG Lin-feng | Department of Radiology, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai, 200080, China | | | DUAN Meng | Department of Instrument Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China | | | LI Mao-lin | Department of Radiology, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai, 200080, China | | | JI Ying | Department of Radiology, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai, 200080, China | | | GAO Guo | Department of Instrument Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China | | | LI Kang-an | Department of Radiology, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai, 200080, China | |
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| 中文摘要: |
| 摘要 目的：探讨Fe3O4-PEG-CD56/Avastin@Ce6靶向探针与NK92细胞的结合能力并进行细胞体外MRI成像。方法：制备Fe3O4-PEG-CD56/Avastin@Ce6纳米探针，对合成的材料进行表征。应用凋亡试剂盒测定不同浓度的材料对NK92的细胞毒性，通过流式细胞术分析纳米材料与NK92细胞的结合能力和应用MRI对细胞进行体外成像并分析其T2信号强度的改变。结果：合成的纳米探针具有较好的生物相容性，且对NK92细胞的影响较小，不同浓度下细胞凋亡水平基本一致，与NK92细胞结合的材料随浓度的增加而逐渐增加。MRI检查提示不同浓度探针孵育的NK92细胞T2加权像(T2WI)的信号均降低。结论：Fe3O4-PEG-CD56/Avastin@Ce6探针对NK92细胞具有靶向性，3.0 T MR扫描仪可对其进行体外监测。 |
| 英文摘要: |
| ABSTRACT Objective: To explore ability of Fe3O4-PEG-CD56/Avastin @ Ce6 targeted probe to bind to NK92 cells and perform MRI to cells in vitro. Methods: The nanoprobes of Fe3O4-PEG-CD56/Avastin@Ce6 were prepared and were characterized. Then the cytotoxicity of this nano-materials to NK92 was determined by apoptotic kit and the cellular uptake of nanomaterials to NK92 cells was analyzed by flow cytometry. Finally, MRI was used to research the signal intensity of cells. Results: The nanoprobes with good biocompatibility were synthesized successfully. The cytotoxicity of the nanoprobe was low and the apoptosis levels were basically the same at different concentrations for NK92 cells. Flow cytometry showed that the nanomaterials bound to NK92 cells increased with the raised concentration. MRI showed that the signal of T2-weighted image (T2WI) was declined significantly with the rise of Fe3O4-PEG-CD56/Avastin@Ce6 concentrations. Conclusion: The Fe3O4-PEG-CD56/Avastin@Ce6 nanoprobe has potential to targeting NK92 cells and can monitor using the 3.0T MR scanner in vitro. |
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