| 罗绮薇,蔡燕丽,黄炎珍,陈伟意,严红金,林汉标,马天宝,许 超,钟惠敏,魏 伟.采集到制备的不同时长对脐带血干细胞冻前质量的影响[J].现代生物医学进展英文版,2020,(14):2609-2613. |
| 采集到制备的不同时长对脐带血干细胞冻前质量的影响 |
| Impact of Time Interval from Collection to Processing on Quality of Umbilical Cord Blood |
| Received:January 23, 2020 Revised:February 17, 2020 |
| DOI：10.13241/j.cnki.pmb.2020.14.002 |
| 中文关键词: 脐带血 冻存 有核细胞活性 时长 质量 |
| 英文关键词: Umbilical cord blood Cryopreservation Viability of nucleated cells Time interval Quality |
| 基金项目:广东省科技计划项目（2015B020227003） |
| Author Name | Affiliation | E-mail | | LUO Qi-wei | 1 The Second Clinical Medical College of Southern Medical University, Guangzhou, Guangdong, 510515, China
2 Department of Obstetrics and Gynecology, He Xian Memorial Hospital of Guangzhou Panyu District, Guangzhou, Guangdong, 511400, China | gdcbbkeyan@163.com | | CAI Yan-li | Guangdong Experimental Center of Cord Blood Stem Cell Bank, Guangzhou, Guangdong, 510663, China | | | HUANG Yan-zhen | Department of Obstetrics and Gynecology, He Xian Memorial Hospital of Guangzhou Panyu District, Guangzhou, Guangdong, 511400, China | | | CHEN Wei-yi | Department of Obstetrics and Gynecology, He Xian Memorial Hospital of Guangzhou Panyu District, Guangzhou, Guangdong, 511400, China | | | YAN Hong-jin | Guangdong Experimental Center of Cord Blood Stem Cell Bank, Guangzhou, Guangdong, 510663, China | | | LIN Han-biao | Guangdong Experimental Center of Cord Blood Stem Cell Bank, Guangzhou, Guangdong, 510663, China | | | MA Tian-bao | Guangdong Experimental Center of Cord Blood Stem Cell Bank, Guangzhou, Guangdong, 510663, China | | | XU Chao | Guangdong Experimental Center of Cord Blood Stem Cell Bank, Guangzhou, Guangdong, 510663, China | | | ZHONG Hui-min | Department of Obstetrics, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, 510260, China | | | WEI Wei | Guangdong Experimental Center of Cord Blood Stem Cell Bank, Guangzhou, Guangdong, 510663, China | |
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| 中文摘要: |
| 摘要 目的：分析采集到制备不同的时长对脐带血干细胞的冻前相关质量指标的影响，为确定合适的采集到制备时长提供依据。方法：选取1712份脐带血样本，按照采集到制备的时长分为<12 h组、(12-24) h组、(24-36) h组和(36-48) h组，分析脐带血干细胞冻前有核细胞数量、有核细胞活性、CD34+细胞百分比及CFU-GM集落数的变化。结果：（1）冻前有核细胞数量随着时间延长而下降，但各组冻前有核细胞数量的差异无统计学意义(P>0.05)；（2）各组的有核细胞活性结果比较发现，(12-24) h组和(24-36) h组的有核细胞活性差异无统计学意义（P>0.05），其余各组间有核细胞活性比较差异均有统计学意义(P<0.05)；（3）CD34+细胞百分比随时间延长有微弱的升高趋势，但是差异无统计学意义（P>0.05）；（4）各组的CFU-GM集落数比较差异均无统计学意义(P>0.05)。结论：随着脐带血采集到制备的时长增加，有核细胞活性会逐渐下降。脐带血采集后在36小时内制备是安全的，其干细胞的质量指标可稳定保持在较高水平， 脐带血在36-48小时制备虽然质量有微弱下降，其干细胞的冻前质量也满足临床出库需求。 |
| 英文摘要: |
| ABSTRACT Objective: In order to provide the appropriate time intervals from collection to processing of umbilical cord blood (UCB), we analyzing the relevant quality indicators from distinct groups of time intervals and its relationship. Methods: The amount of 1712 UCBs divided into distinct groups based on different time interval (each 12 hours increment) were assessed by the indicators including pre-cryopreservation total nucleated cells, viability of nucleated cells, CD34+ cell rates and CFU-GM. Results: (1) The pre-cryopreservation total nucleated cells declared due to the extension of transportation time, and there was no statistical difference (P>0.05). (2) The viability of nucleated cells was affected by time interval between collection and processing, there was no significant difference in the activity of nuclear cells between (12-24) h group and (24-36) h group (P>0.05), and there was significant difference in the activity of nuclear cells between the other groups (P<0.05). (3) The CD34+ % had an slight upward trend with the extension of time interval without statistical difference (P＞0.05). (4) CFU-GM was not affected by time interval from collection to processing in each group (P＞0.05). Conclusion: The extension of the time from collection to processing of UCBs can reduce the quality by decreasing cell viability. UCBs was safe to cryopreservation within 36 hours, and its related quality indicators can be stably maintained at a high level. Though the quality of UCBs was slightly decreased in 36-48 hours, it was still fit the standards for clinical transplantation. |
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