| 李姝蒙,刘忠瀚,俞 邦,孙 悦,朱泠霏,武慧超,朱明瑾,毛 萌.二十味沉香丸对卒中后PTSD模型大鼠行为学及5-HT1AR的调节作用探析[J].现代生物医学进展英文版,2020,(11):2019-2023. |
| 二十味沉香丸对卒中后PTSD模型大鼠行为学及5-HT1AR的调节作用探析 |
| Regulatory Effect of Ershiwei Chenxiang Pill on Behavior and 5-HT1AR in PTSD Rats after Stroke |
| Received:December 30, 2019 Revised:January 24, 2020 |
| DOI：10.13241/j.cnki.pmb.2020.11.004 |
| 中文关键词: 卒中后PTSD 二十味沉香丸 5-HT1AR |
| 英文关键词: PTSD after Stroke Ershiwei Chenxiang pill 5-HT1AR |
| 基金项目:青年教师项目基金课题（2019-Jyb-JS-001）；国家级大学生创新创业训练项目（201910026006）；国家自然科学基金青年基金项目（81403498） |
| Author Name | Affiliation | E-mail | | LI Shu-meng | Beijing University of Chinese Medicine, Beijing, 100029, China | 15901013381@163.com | | LIU Zhong-han | Beijing University of Chinese Medicine, Beijing, 100029, China | | | YU Bang | Beijing University of Chinese Medicine, Beijing, 100029, China | | | SUN Yue | Beijing University of Chinese Medicine, Beijing, 100029, China | | | ZHU Ling-fei | Beijing University of Chinese Medicine, Beijing, 100029, China | | | WU Hui-chao | Beijing University of Chinese Medicine, Beijing, 100029, China | | | ZHU Ming-jin | Beijing University of ChineseMedicine, Third Affiliated Hospital, Beijing, 100029, China | | | MAO Meng | Beijing University of Chinese Medicine, Beijing, 100029, China | |
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| 中文摘要: |
| 摘要 目的：探究藏药二十味沉香丸对卒中后PTSD模型大鼠行为和5-HT1AR的调节作用。方法：将48只大鼠随机分为假手术组、模型组、二十味沉香丸组、帕罗西汀组。每组各12只。造模后未达到观察时点死亡的大鼠排除本研究，筛选出符合条件的大鼠并通过随机抽样原则补齐动物。造模后每天分别给予相应的药物或生理盐水进行灌胃，灌胃2周后采用旷场实验检测各组剩余大鼠行为学变化。每组各取6只，采用实时荧光定量PCR和 Western blot 法分别检测海马组织中5-HT1AR基因和蛋白水平的变化。结果：与假手术组相比较，模型组运动总距离（P<0.01）、单次最大运动距离（P<0.05）、穿格次数（P<0.01）、直立次数（P<0.01）、直立时间（P<0.01）、修饰时间（P<0.01）、修饰次数（P<0.05）减少差异有统计学意义，5-HT1AR基因（P<0.05）及蛋白表达水平下降；与模型组相比较，二十味沉香丸组运动总距离（P<0.05）、穿格次数（P<0.05）、直立时间（P<0.05）、直立次数（P<0.01）增加差异具有统计学意义，且5-HT1AR的基因（P<0.05）及蛋白水平表达升高；与模型组相比较，帕罗西汀组穿格次数（P<0.05）、运动总距离（P<0.05）、直立时间（P<0.05）增加差异具有统计学意义，5-HT1AR的基因（P<0.01）及蛋白表达水平升高。结论：本实验成功复制了卒中后PTSD模型，所选取的中药二十味沉香丸能在一定程度上改善卒中后PTSD模型大鼠的焦虑样行为，其效用与西药帕罗西汀类似，且作用机制可能与调节5-HT1AR有关。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effect of the Tibetan medicine ErShiwei Chenxiang Pill on the regulation of rat behavior and 5-HT1AR in PTSD model after stroke. Methods: 48 rats were randomly divided into sham operation group, model group, Ershiwei Chenxiang pill group and paroxetine group. There are 12 rats in each group. The rats that did not die at the observation time after modeling were excluded from this study, and the qualified rats were screened out and the animals were supplemented by the principle of random sampling. After the model was established, the corresponding drugs or saline were given intragastrically every day. 2 weeks later, the behavioral changes of the remaining rats in each group were detected by open field test. Each group has its own The changes of 5-HT1AR gene and protein in hippocampus were detected by real-time fluorescence quantitative PCR and Western blot, respectively. Results: Compared with the sham group, data of the model group, the total distance (P<0.01), the single maximum movement distance (P<0.05), times of spanning lattice (P<0.01), vertical activity (P<0.01), the vertical time (P<0.01), the time of modification (P<0.01) and the number of modification (P<0.05) were of statistical significance. The expression level of 5-HT1AR gene was of statistical significance (P <0.05), and the level of protein expression was decreased. Compared with the model group, the data of the ESWCX, the total distance（P<0.05）, times of spanning lattice（P<0.05） the vertical time (P<0.05) and vertical activity (P<0.01), and the expression of 5-HT1AR gene(P<0.05) and protein level were increased. Compared with the model group, paroxetine group had a statistically significant difference in the number of times of penetration (P<0.05), the total distance (P<0.05), and the vertical time (P<0.05). The gene of 5-HT1AR (P<0.01) and the level of protein expression were increased. Conclusion: This experiment has successfully copied the PTSD model after stroke, and the selected Chinese medicine Ershiwei Chenxiang pill can be used to improve the anxiety-like behavior of the PTSD model in the post-stroke PTSD model, the utility is similar to that of the western medicine paroxetine, and the mechanism of action may be related to the regulation of 5-HT1AR. |
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