| 陈怡环,薛姗姗,顾婷婷,王化宁,彭正午.电针早期干预对创伤后应激模型大鼠的行为及前额叶皮质BDNF、IL-1β和IL-6水平的影响[J].现代生物医学进展英文版,2020,(9):1619-1623. |
| 电针早期干预对创伤后应激模型大鼠的行为及前额叶皮质BDNF、IL-1β和IL-6水平的影响 |
| Effect of Early Intervention with Electroacupuncture on Anxiety-like Behavior and Expression of BDNF, IL-1β and IL-6 in the Prefrontal Cortex of PTSD Rats Model |
| Received:October 28, 2019 Revised:November 24, 2019 |
| DOI：10.13241/j.cnki.pmb.2020.09.004 |
| 中文关键词: 电针 脑源性神经营养因子 白细胞介素-6 创伤后应激障碍 |
| 英文关键词: Electroacupuncture Brain-derived neurotrophic factor Interleukin-6 Posttraumatic stress disorder |
| 基金项目:国家自然科学基金项目(81571309) |
| Author Name | Affiliation | E-mail | | CHEN Yi-huan | Department of Psychosomatic, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | chenyihuan12345@163.com | | XUE Shan-shan | Department of Psychosomatic, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | | | GU Ting-ting | Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | | | WANG Hua-ning | Department of Psychosomatic, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | | | PENG Zheng-wu | Department of Psychosomatic, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | |
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| 中文摘要: |
| 摘要 目的：观察电针（Electroacupuncture，EA）早期干预对创伤后应激（Posttraumatic stress disorder，PTSD）模型大鼠的焦虑样行为及前额叶皮质（Prefrorntal cortex，PFC）中脑源性神经营养因子（Brain-derived neurotrophic factor，BDNF）、白介素1β（Interleukin-1beta，IL-1β）和白介素6（Interleukin-6，IL-6）水平的影响。方法：将32只雄性SD大鼠经环境适应后，随机分为Sham组、Sham + EA组、ESPS组和ESPS + EA组，每组8只。对ESPS组和ESPS + EA组大鼠进行增强型单次延长应激（Enhanced single prolonged stress，ESPS）造模处理，其他两组不接受ESPS，但是置于同一实验室环境。造模结束后24 h，各组大鼠进行EA干预：Sham + EA组和ESPS + EA组的大鼠每天接受EA刺激（百会穴，1 mA，2/15 Hz）30 min，连续1周；另外两组给予假刺激（无电流）每天30 min，连续1周。静置一周后，采用旷场和高架十字实验观察各组大鼠的行为，之后处死大鼠，分别用蛋白质印迹法和酶联免疫法（Enzyme-linked immunosorbent assay，ELISA）检测各组大鼠PFC中BDNF的表达水平以及IL-1β和IL-6的水平。结果：（1）ESPS处理导致大鼠焦虑样行为，在旷场中心区运动距离和探索时间百分比减少，在高架十字开臂运动距离及停留时间百分比减少。ESPS组大鼠PFC中BDNF表达下降，IL-1β和IL-6的水平升高；（2）EA早期干预可以改善大鼠的焦虑样行为，提高ESPS模型大鼠PFC中BDNF的表达，降低IL-6的水平，对IL-1β的影响无统计学差异。结论：EA早期干预改善了ESPS诱导的大鼠焦虑样的行为，这可能与其增加了PFC中BDNF表达，降低了炎性因子IL-6的表达有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of early intervention with Electroacupuncture (EA) on the anxiety-like behavior of ESPS-treated rat and the regulation of BDNF, IL-1β and IL-6 in the prefrontal cortex (PFC). Methods: Thirty - two male Sprague - Dawley rats were randomly divided into Sham, Sham + EA, ESPS and ESPS + EA group after adjusting the environment for 1 week. Rats in ESPS group and ESPS + EA group were exposed to ESPS, and rats in Sham and Sham + EA groups were placed in the same experimental environment but did not receive ESPS at the same time. 24 h later, rats in Sham + EA group and ESPS + EA group received EA treatment (Baihui, 1 mA, 2/15 Hz) and the other two groups received sham stimulation (acupuncture treatment without electricity) for 30 min every day for 1 week. The open field test and elevated-plus maze test were performed 1 week later. Then all rats were sacrificed and the level of BDNF and the level of IL-1β and IL-6 in PFC were measured by Western blot and ELISA, respectively. Results: (1) The traveled distance and the percentage of exploration time in central region of open field test and the percentage of exploration time in open arms of elevated-plus maze test were significantly decreased in ESPS group than that of other groups. The expression of BDNF in PFC was decreased, while the levels of IL-1β and IL-6 were increased in the ESPS group than that of other groups. (2) Early intervention of EA ameliorated the anxiety-like behavior of rats, elevated the expression of BDNF and reduced the level of IL-6 in PFC of ESPS-treated rats. However, there was no significant different between ESPS group and ESPS + EA group on the level of IL-1β. Conclusion: Early intervention with EA improved ESPS-induced anxiety-like behavior in rats, which might be related to the increase of BDNF expression and decrease of the level of IL-6 in PFC. |
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