| 暴 鹏,李 雪,孙丽莎,张晓冉,杨欣怡,李朝敏.天阳复脉汤对糖尿病周围神经病变小鼠临床疗效及其对JNK和p-JNK蛋白表达的影响[J].现代生物医学进展英文版,2020,(8):1436-1440. |
| 天阳复脉汤对糖尿病周围神经病变小鼠临床疗效及其对JNK和p-JNK蛋白表达的影响 |
| Clinical Effect of Tianyang Fumai Decoction on Diabetic Peripheral Neuropathy in Mice and Its Effect on the Expression of JNK and p-JNK Protein |
| Received:August 23, 2019 Revised:September 18, 2019 |
| DOI：10.13241/j.cnki.pmb.2020.08.008 |
| 中文关键词: 天阳复脉汤 糖尿病周围神经病变 临床疗效 c-jun氨基末端激酶 磷酸化c-jun氨基末端激酶 |
| 英文关键词: Tianyang Fumai decoction Diabetic peripheral neuropathy Clinical efficacy C-jun N-terminal kinase Phosphorylated c-jun N-terminal kinase |
| 基金项目:国家自然科学基金项目 （81870544） |
| Author Name | Affiliation | E-mail | | BAO Peng | Department of Endocrinology, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, 610075, China | chenya654@sina.com | | LI Xue | Department of Endocrinology, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, 610075, China | | | SUN Li-sha | Department of Endocrinology, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, 610075, China | | | ZHANG Xiao-ran | Department of Endocrinology, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, 610075, China | | | YANG Xin-yi | Department of Endocrinology, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, 610075, China | | | LI Zhao-min | Department of Endocrinology, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, 610075, China | |
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| 中文摘要: |
| 摘要 目的：探索天阳复脉汤对糖尿病周围神经病变小鼠临床疗效及其对JNK和p-JNK蛋白表达的影响。方法：选用7周的db/db雄性小鼠120只，随机分成模型组、硫辛酸组和天阳复脉汤高、中、低剂量组等五组，每组24只。选取同窝生7周龄db/m雄性小鼠24只为正常组。模型组和正常组给予等体积蒸馏水，而硫辛酸组灌胃给药，剂量为78 mg/kg，高剂量组为6.8 g/kg、中剂量组为3.4 g/kg、低剂量组为1.7 g/kg。对比和评估六组小鼠足底热敏反应时间、运动神经传导速度、血清因子及JNK及p-JNK蛋白表达等指标。结果：与正常组和模型组相比，硫辛酸组和天阳复脉汤组小鼠足底热敏反应时间均显著缩短，MNCV速度均显著提高，血清TNF-α和IL-1β水平明显降低，JNK和p-JNK蛋白表达显著下降，差异均具有统计学意义(P<0.05)。结论：天阳复脉汤应用于糖尿病周围神经病变小鼠，可有效增加其运动神经传导速度，缩短其足底热敏反应时间，降低血清TNF-α和IL-1β浓度，进而下调JNK和p-JNK蛋白表达。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the clinical effect of Tianyang Fumai decoction on diabetic peripheral neuropathy in mice and its effect on the expression of JNK and p-JNK protein. Methods: 120 cases of mice were selected as the observation subjects, they were divided into the model group, lipoic acid group and Tianyang Fumai decoction high, middle and low dose group according to the random table method, 24 cases were in each group. Besides, 24 7-week-old db/m male mice born in the same litter were selected as normal group. The plantar thermal response time, motor nerve conduction velocity, serum factor and the expression of JNK and p-JNK protein were compared and evaluated in the six groups. Results: Compared with the normal group and the model group, the plantar thermosensitive reaction time was significantly shortened, the MNCV speed was significantly increased, the serum TNF-α and IL-1β levels were significantly decreased, and the expression of JNK and p-JNK protein were significantly decreased in the lipoic acid group and Tianyang Fumai decoction group, the differences were statistically significant (P<0.05). Conclusion: Tianyang Fumai decoction in the treatment of diabetic peripheral neuropathy mice can effectively increase the motor nerve conduction velocity, shorten the plantar thermosensitive reaction time, reduce the concentration of serum TNF-α and IL-1β, and then down-regulate the expression of JNK and p-JNK protein. |
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