| 李凤晓,史雪莹,初向华,卢晓东,董加花,于良健,张 宁.钙敏感受体在大鼠脑缺血再灌注损伤时细胞凋亡中的作用[J].现代生物医学进展英文版,2020,(6):1043-1046. |
| 钙敏感受体在大鼠脑缺血再灌注损伤时细胞凋亡中的作用 |
| Role of Calcium Sensing Receptor on Cerebral Ischemia-reperfusion Injury in Rats |
| Received:July 07, 2019 Revised:July 30, 2019 |
| DOI：10.13241/j.cnki.pmb.2020.06.009 |
| 中文关键词: 受体 钙敏感 再灌注损伤 脑 细胞凋亡 |
| 英文关键词: Receptors calcium-sensing Reperfusion injury Brain Apoptosis |
| 基金项目:国家自然科学基金项目（81771415） |
| Author Name | Affiliation | E-mail | | LI Feng-xiao | Department of Pharmacy, Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266003, China | blws512@126.com | | SHI Xue-ying | Department of Pharmacy, Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266003, China | | | CHU Xiang-hua | Department of Pharmacy, Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266003, China | | | LU Xiao-dong | Department of Pharmacy, Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266003, China | | | DONG Jia-hua | Department of Pharmacy, Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266003, China | | | YU Liang-jian | Department of Pharmacy, Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266003, China | | | ZHANG Ning | Department of Anesthesiology, Qingdao Municipal Hospital, Qingdao, Shandong, 266011, China | |
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| 中文摘要: |
| 摘要 目的：评价钙敏感受体在大鼠脑缺血再灌注损伤时细胞凋亡中的作用。方法：健康成年雄性Wistar大鼠60只，体重250~300 g，采用随机数字表法分为3组（n=20）：假手术组(S组)、脑缺血再灌注组(I/R组)和钙敏感受体拮抗剂组(N组)。I/R组和N组采用线栓法经左侧颈外-颈内动脉插线制备大鼠脑缺血再灌注损伤模型，于脑缺血前10 min尾静脉注射等容量二甲基亚砜和钙敏感受体拮抗剂NPS-89636 1 mg/kg。于再灌注24 h时行神经功能评分，随后处死大鼠取脑组织，测定MDA含量和SOD活性，采用TUNEL法观察神经细胞凋亡情况，计算神经细胞凋亡指数，免疫组化法检测Caspase-3阳性细胞的表达，Western blot法检测Caspase-3蛋白的表达。结果：I/R组和N组MDA含量、神经细胞凋亡指数、Caspase-3阳性细胞和Caspase-3蛋白表达水平高于S组，神经功能评分和SOD活性低于S组，差异有统计学意义(P<0.05)；N组MDA含量、神经细胞凋亡指数、Caspase-3阳性细胞和Caspase-3蛋白表达水平低于I/R组，神经功能评分和SOD活性高于I/R组，差异有统计学意义(P<0.05)。结论：钙敏感受体参与大鼠脑缺血再灌注损伤和细胞凋亡的发生。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of calcium sensing receptor on cerebral ischemia-reperfusion (I/R)in rats. Methods: Sixty male Wistar rats, weighing 250-300 g, were randomly divided into three groups（n=20 each）using a random number table: sham operation group(group S), group I/R and calcium-sensing receptor antagonists group (group N). Animal models of middle cerebral artery occlusion/reperfusion in the later 2 groups were established by a filament method in the left external-internal carotid artery. Rats were injected with 1 mg/kg NPS-89636 in the calcium-sensing receptor antagonists group and the equal volume of dimethyl sulfoxide 10 minutes before the cerebral ischemia. Neurological function was assessed and scored at 24 h of reperfusion. The rats were sacrificed and then the brains were removed for determination of MDA content, SOD activity, apoptotic cell death was assessed by TUNEL staining，and the expression of Caspase-3 by immunohistochemical and Western blot. Results: The content of MDA, neuronal apoptosis index, expression levels of Caspase-3 positive cells and Caspase-3 protein in group I/R and N were higher than those in group S, and neurological function score and SOD activity were lower than those in group S(P<0.05); The content of MDA, neuronal apoptosis index, expression levels of Caspase-3 positive cells and Caspase-3 protein in group N were lower than those in group I/R, and neurological function score and SOD activity were higher than those in group I/R(P<0.05). Conclusion: Calcium-sensing receptor is involved in cerebral ischemia-reperfusion injury and cell apoptosis. |
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