王若男,赵德喜,孙晓舟,崔洪玉,吴九如.红细胞诱导BV-2小胶质细胞制作脑出血吞噬模型的研究[J].现代生物医学进展英文版,2020,(6):1038-1042. |
红细胞诱导BV-2小胶质细胞制作脑出血吞噬模型的研究 |
RBC Induced Microglia of BV-2 to Produce Intracerebral Hemorrhage Phagocytosis Model |
Received:October 27, 2019 Revised:November 22, 2019 |
DOI:10.13241/j.cnki.pmb.2020.06.008 |
中文关键词: 红细胞 小胶质细胞 脑出血 吞噬 |
英文关键词: RBC BV-2 Intracerebral Hemorrhage Swallowing |
基金项目:国家自然科学基金面上项目(81774224);吉林省教育厅"十三五"科学技术项目(JJKH20181247KJ) |
Author Name | Affiliation | E-mail | WANG Ruo-nan | Changchun University Of Chinese Medicine the college of traditional Chinese medicine, Changchun, Jilin, 130117, China | 871684963@qq.com | ZHAO De-xi | Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, Jilin, 130021, China | | SUN Xiao-zhou | Changchun University Of Chinese Medicine the college of traditional Chinese medicine, Changchun, Jilin, 130117, China | | CUI Hong-yu | Changchun University Of Chinese Medicine the college of traditional Chinese medicine, Changchun, Jilin, 130117, China | | WU Jiu-ru | Changchun University Of Chinese Medicine the Acupuncture and massage, Changchun, Jilin, 130000, China | |
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中文摘要: |
摘要 目的:观察不同储存时间、不同比例的红细胞(RBC)与小鼠BV-2小胶质细胞共培养不同时间对BV-2吞噬RBC的影响。方法:将用阿氏液保存1天、7天、14天、21天、30天的小鼠RBC按照5:1、10:1、20:1、40:1的比例加入BV-2小胶质细胞中,共培养1 h、3 h、6 h、9 h, 应用流式细胞术观察 BV-2对于RBC的吞噬情况。结果:加入不同保存时间的RBC与BV-2小胶质细胞共培养均可见到吞噬情况,其中保存28天的效果最明显;加入不同比例的RBC与BV-2小胶质细胞共培养,均出现吞噬情况,其中40:1最明显;RBC与BV-2小胶质细胞共培养各时间点均可观察到吞噬情况,其中6小时最明显。结论:应用RBC诱导小鼠BV-2小胶质细胞制作大鼠脑出血体外模型,选择保存28天RBC与BV-2小胶质细胞的比例为40:1共培养6小时,此时BV-2小胶质细胞吞噬清除RBC的效果最明显。 |
英文摘要: |
ABSTRACT Objective: Observe the influences that BV-2, co-cultured with mice' glial cell for different time, exerts to the swallowing RBC with different storage time and different proportion. Methods:Store mice' RBC with flow cytometry for 1 day, 7 days, 14 days, 21 days and 30 days respectively. Add the stored RBC into BV-2 glial cell with the proportion of 5:1, 10:1, 20:1 and 40:1. Co-culture them for 1 h, 3 h, 6 h, 9 h, and observe the swallowing condition between BV-2 and RBC. Results: The swallowing phenomenon can be observed after adding RBC with different stored time into BV-2 glial cell. The phenomenon is the most obvious after storing for 28 days; After co-culturing BV-2 and RBC with different proportion, the swallowing phenomenon is also observed. In which the phenomenon is the most obvious with the proportion of 40:1. The swallowing phenomenon can be observed in each co-culture time, in which the phenomenon is the most obvious after 6 h. Conclusion: Apply RBC to induce BV2 glial cell making in vitro intracerebral hemorrhage model. Choose RBC which stored for 28 days and co-culture it with BV-2 for 6h with proportion of 40:1. Thus, the swallowing phenomenon between BV2 glial cell and RBC is the most obvious. |
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