| 戴金芬,吴鹏波,朱 姗,王 波,胡 松,陈 怡.汉黄芩素通过调控上皮间质转化抑制胃癌细胞的侵袭转移[J].现代生物医学进展英文版,2020,(6):1022-1027. |
| 汉黄芩素通过调控上皮间质转化抑制胃癌细胞的侵袭转移 |
| Wogonin Inhibition of Cell Migration and Invasion in Human Gastric Cancer by Disruption of Epithelial-mesenchymal Transition |
| Received:October 08, 2019 Revised:October 31, 2019 |
| DOI：10.13241/j.cnki.pmb.2020.06.005 |
| 中文关键词: 汉黄芩素 胃癌 EMT 迁移 侵袭 |
| 英文关键词: Wogonin Gastric cancer Epithelial-mesenchymal transition Migration Invasion |
| 基金项目:湖北省科技厅青年基金项目（2018CFB236） |
| Author Name | Affiliation | E-mail | | DAI Jin-fen | Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China | brilliant_510@126.com | | WU Peng-bo | Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China | | | ZHU Shan | Department of Thyroid Breast Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China | | | WANG Bo | Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China | | | HU Song | Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China | | | CHEN Yi | Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China | |
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| 中文摘要: |
| 摘要 目的：汉黄芩素是中药黄芩中的一种黄酮，具有体内外抗癌活性。然而，汉黄芩素对人胃癌细胞的作用尚不十分清楚。本研究拟探讨汉黄芩素对人胃癌细胞MGC-803侵袭转移能力的影响及其对上皮间质转化（Epithelial to Mesenchymal Transition, EMT）的作用机制。方法：采用MTT法测定汉黄芩素对人胃癌细胞MGC-803增殖能力的影响，通过划痕实验、Transwell试验检测汉黄芩素对人胃癌细胞MGC-803迁移、侵袭能力的影响。通过免疫印迹法和免疫荧光法分析汉黄芩素对EMT的影响。结果：20 μM以上浓度的汉黄芩素能抑制人胃癌细胞MGC-803的增殖，不同浓度的汉黄芩素能抑制人胃癌细胞MGC-803的迁移和侵袭，且呈浓度依赖性。此外，汉黄芩素能抑制间质标记蛋白波形蛋白(Vimentin)和锌指蛋白E-盒结合同源异形盒-1(ZEB1)的表达，促进 上皮标记蛋白E-钙黏蛋白(E-cadherin）的表达。结论：汉黄芩素能抑制胃癌细胞的侵袭和迁移，这一作用可能与其抑制EMT的发生有关。 |
| 英文摘要: |
| ABSTRACT Objective: Wogonin, a flavone identified in Scutellariae radix, shows in vitro and in vivo anti-cancer properties. However, the mechanism of action of wogonin on human gastric cancer (GC) cells remains unclear. Herein, this strudy was performed to investigate the influence of wogonin on cell migration and invasion in human GC cells MGC803 and its role in epithelial-mesenchymal transition (EMT). Methods: Effection on cell proliferation of human GC cells MGC803 was assessed by 3-(4,5)-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Wound-healing, transwell migration, and invasion assays were carried out to determine changes in MGC-803 cell migration and invasion. The influence of the wogonin on EMT was analyzed by both Western blotting and immunofluorescence. Results: Wogonin with the concentration of more than 20 ?滋M effectively suppressed the proliferation of human GC MGC-803 cells. Different concentrations of wogonin inhibited the migration and invasion of human GC MGC-803 cells in a concentration-dependent manner. Furthermore, the application of wogonin resulted in the down-regulation of the mesenchymal biomarkers vimentin and ZEB1 but up-regulated the epithelial biomarker E-cadherin. Conclusion: Our findings indicate that wogonin imparts inhibitory effects on cell invasion and migration in GC, possibly by disrupting EMT. |
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