| 胡 斌,王成举,杨 望,常 琴,屈福祥,陈鹏慧,张雨平.缺氧缺血诱导对原代新生大鼠前脑混合细胞髓鞘形成和细胞骨架的影响[J].现代生物医学进展英文版,2020,(4):624-628. |
| 缺氧缺血诱导对原代新生大鼠前脑混合细胞髓鞘形成和细胞骨架的影响 |
| Effect of Hypoxic-ischemic-induced on Myelin Formation and Cytoskeleton of Mixed Forebrain Cells in Newborn Rats |
| Received:May 28, 2019 Revised:June 23, 2019 |
| DOI：10.13241/j.cnki.pmb.2020.04.005 |
| 中文关键词: 缺氧缺血诱导 髓鞘 细胞骨架 前脑混合细胞 |
| 英文关键词: Hypoxic-ischemic-induced Myelination Cytoskeleton Mixed forebrain cells |
| 基金项目:国家自然科学基金项目（81671496） |
| Author Name | Affiliation | E-mail | | HU Bin | Department of Pediatrics, Second Affiliated Hospital of Army Medical University, Chongqing, 400037, China | 58127652@qq.com | | WANG Cheng-ju | Department of Pediatrics, Second Affiliated Hospital of Army Medical University, Chongqing, 400037, China | | | YANG Wang | Department of Pediatrics, Second Affiliated Hospital of Army Medical University, Chongqing, 400037, China | | | CHANG Qin | Department of Pediatrics, Second Affiliated Hospital of Army Medical University, Chongqing, 400037, China | | | QU Fu-xiang | Department of Pediatrics, Second Affiliated Hospital of Army Medical University, Chongqing, 400037, China | | | CHEN Peng-hui | Department of Neurobiology, School of Basic Medical Sciences, Army Medical University, Chongqing, 400038, China | | | ZHANG Yu-ping | Department of Pediatrics, Second Affiliated Hospital of Army Medical University, Chongqing, 400037, China | |
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| 中文摘要: |
| 摘要 目的：该研究探讨了缺氧缺血诱导对新生大鼠前脑混合细胞髓鞘形成和细胞骨架的影响。方法：在原代培养的新生大鼠前脑混合细胞中，应用免疫组化染色髓鞘碱性蛋白（MBP）和劳克坚牢蓝（LFB）染色检测髓鞘和轴突的发育情况；Western印迹分析NF155蛋白的表达情况；免疫荧光分析参与细胞骨架调节的ERM-Rho GTP酶家族相关蛋白表达。结果：缺氧缺血对混合细胞的进一步分化和成熟起到抑制作用，MBP染色阳性率降低57 %，髓鞘和轴突染色阳性率降低74 %；NF155蛋白表达降低51 %；Rho GTP酶家族成员Rac1、Cdc42分别降低81 %和75 %。结论：缺氧缺血使细胞突起的形成和骨架重组受到阻碍，继而影响髓鞘的发育和成熟，这一过程与ERM-Rho GTP酶细胞骨架通路有关。 |
| 英文摘要: |
| ABSTRACT Objective: The aim of this work was to investigate the effects of hypoxic ischemic on myelin formation and cytoskeleton of mixed forebrain cells in newborn rats. Methods: The expression of myelin development-related proteins myelin basic protein was verified by immunohistochemical staining and Luxol fast blue staining was used to detect the development of myelin sheath and axon in primary cultured mixed forebrain cells; the expression of protein NF155 was verified by Western blotting; the expression of ERM-Rho GTPase related proteins involved in cytoskeleton were verified by immunofluorescence analysis. Results: The results showed that hypoxic ischemic inhibited the further differentiation and maturation of mixed cells. The mean density of MBP, myelin sheath and axon, NF155, Rac1 and Cdc42 were decreases 57 %, 74 %, 51 %, 81 % and 75 % resperctively. Conclusion: In conclusion the formation of cell processes, the recombination of cytoskeleton were hindered by hypoxic-ischemic-induced, which affected the development and maturation of myelin sheath, and this process is related to the obstruction of cytoskeleton network ERM-Rho GTase signal pathway. |
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