Article Summary
章 萧,艾 芬,张莉红,刘 迪,张佩雯.微小RNA-30e调控EMT对胃癌侵袭和迁移影响的研究[J].现代生物医学进展英文版,2019,19(22):4208-4212.
微小RNA-30e调控EMT对胃癌侵袭和迁移影响的研究
Effects of microRNA-30e Regulating Epithelial Mesenchymal Transition on Invasion and Migration in Gastric Cancer
Received:May 08, 2019  Revised:May 30, 2019
DOI:10.13241/j.cnki.pmb.2019.22.002
中文关键词: 胃癌  上皮细胞间充质转化  微小RNA-30e  侵袭  迁移
英文关键词: Gastric cancer  EMT  miR-30e  Invasion  Migration
基金项目:湖北省卫生计生委科研项目(WJ2016MB007)
Author NameAffiliationE-mail
ZHANG Xiao Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science and Technology, Wuhan, Hubei, 430024, China zhangxiaoisis@163.com 
AI Fen Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science and Technology, Wuhan, Hubei, 430024, China  
ZHANG Li-hong Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science and Technology, Wuhan, Hubei, 430024, China  
LIU Di Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science and Technology, Wuhan, Hubei, 430024, China  
ZHANG Pei-wen Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science and Technology, Wuhan, Hubei, 430024, China  
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中文摘要:
      摘要 目的:探讨微小RNA-30e(miR-30e)对胃癌细胞迁移和侵袭能力的影响及可能的作用机制。方法:利用Transwell实验和细胞划痕实验检测胃癌细胞系BGC823侵袭和迁移的能力;以脂质体包裹合成miR-30e转染至BGC823细胞,并设空白载体作为对照组;Real-time PCR分别检测实验组和对照组细胞中miR-30e的表达。RT-PCR检测过表达miR-30e后对上皮细胞间充质转化(EMT)相关标记分子Snail、Vimentin、N-cadherin和E-cadherin表达的影响。结果:miR-30e转染至胃癌细胞后,抑制EMT通路主要因子Snail,Vimentin 和N-cadherin mRNA和蛋白质表达,而增加E-cadherin的mRNA和蛋白质表达;miR-30e通过TGF-β对BGC823细胞的侵袭和迁移能力有明显的抑制作用。结论:miR-30e可能是肿瘤细胞EMT过程的关键靶标靶点,阻断EMT过程,可以抑制胃癌细胞的侵袭和迁移能力。
英文摘要:
      ABSTRACT Objective: To investigate the effect of microRNA-30e (miR-30e) on the invasion and migration of gastric cancer and the potential mechanism. Methods: The transwell system and the assays of wound healing were used to assess the invasion and migration ability of gastric cell line BGC823. The miR-30e was transfected into BGC823 cell via lipofectamine 2000. Cells were also transfected with empty vectors to serve as controls. The expression of miR-30e was detected by real-time PCR, and western blot was used to assay the expression of Snail, Vimentin, N-cadherin, and E-cadherin which were the markers of epithelial-mesenchymal transition(EMT). Results: After the transfection of the miR-30e, which had significantly suppressed the major factors in EMT signaling pathway, such as the expression of mRNA and proteins of Snail, N-cadherin, and vimentin, increased the mRNA and proteins expression of E-cadherin. And the mimic also had suppressed the invasion and migration of BGC823 cell line induced by TGF-β. Conclusion: MiR-30e may be a key target in the EMT process of cancer cells, blocking the EMT process could inhibit the invasion and migration of gastric cancer cells.
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