| 章雨微,高晶晶,程 意,韩锋锋,李 刚,罗 勇.不同香烟烟雾暴露强度对大鼠骨骼肌线粒体功能的影响[J].现代生物医学进展英文版,2019,19(17):3208-3212. |
| 不同香烟烟雾暴露强度对大鼠骨骼肌线粒体功能的影响 |
| Effects of Various Intensity Ranks of Cigarette Smoke Exposure on the Mitochondrial Function in the Skeletal Muscles of Rats |
| Received:March 04, 2019 Revised:March 28, 2019 |
| DOI：10.13241/j.cnki.pmb.2019.17.002 |
| 中文关键词: 慢性阻塞性肺疾病 线粒体功能障碍 氧化代谢 骨骼肌功能障碍 |
| 英文关键词: Chronic obstructive pulmonary disease Mitochondrial dysfunction Oxidative metabolism Skeletal muscle atrophy |
| 基金项目:上海市卫生和计划生育委员会资助项目(20134141)；崇明区科学技术委员会科研攻关计划项目（CKY2017-13） |
| Author Name | Affiliation | E-mail | | ZHANG Yu-wei | Department of Respiratory Medicine, Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, 200092, China | zhangyw16@sjtu.edu.cn | | GAO Jing-jing | Department of Respiratory Medicine, Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, 200092, China | | | CHENG Yi | Department of Respiratory Medicine, Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, 200092, China | | | HAN Feng-feng | Department of Respiratory Medicine, Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, 200092, China | | | LI Gang | Department of Respiratory Medicine, Chongming Branch of Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, 202150, China | | | LUO Yong | 1 Department of Respiratory Medicine, Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, 200092, China
2 Department of Respiratory Medicine, Chongming Branch of Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, 202150, China | |
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| 中文摘要: |
| 摘要 目的：探讨不同烟熏暴露强度对大鼠外周骨骼肌线粒体功能的影响。方法：将SD大鼠随机分成4个组，分别接受正常空气或3个周期烟熏暴露(分别是4周、8周、12周)。采用western blot检测大鼠外周骨骼肌与线粒体生成有关的过氧化物酶体增殖物激活受体γ共激活因子1(PGC-1α)以及涉及氧化磷酸化的细胞色素c氧化酶(COX)4的蛋白表达，RT-PCR检测与线粒体氧化代谢有关的Sdhb、线粒体转录因子A(TFAM)的基因表达以及比色法检测琥珀酸脱氢酶(SDH)活性，电镜检测线粒体形态结构的改变。结果：香烟烟雾暴露能够下调大鼠伸趾长肌(EDL)PGC-1α和COX4的蛋白表达，PGC-1α的表达下调呈明显的暴露强度依赖性。香烟烟雾暴露能够诱发大鼠比目鱼肌Sdhb、TFAM基因水平的下调，降低大鼠比目鱼肌SDH活性并呈明显的暴露强度依赖性。电镜显示香烟烟雾暴露诱发伸趾长肌线粒体发生空泡样变性。结论：香烟烟雾暴露诱发大鼠骨骼肌线粒体功能障碍，但该作用与骨骼肌的纤维类型组成无明显相关性。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of cigarette smoke exposure of various intensity ranks on mitochondrial function during skeletal muscles in rats. Methods: Sprague Dawley rats were randomly divided into 4 groups and received normal air or one of three durations of cigarette smoke exposure(4 weeks, 8 weeks and 12 weeks).Western blot was adopted to detect the protein expression of peroxisome-proliferator-activated receptor-γ coactivator-1 (PGC-1α) and cytochrome c oxidase (COX) 4 related to mitochondrial biogenesis and oxidative phosphorylation respectively in peripheral skeletal muscle of rats. And the gene expressions of Sdhb and transcription factor A of mitochondria (TFAM) associated with mitochondrial oxidative metabolism were assayed by RT-PCR. Succinate dehydrogenase (SDH) activity was assessed colorimetrically. Besides, electron microscopy can be uesd for morphological and ultrastructural observation for mitochodria. Results: Cigarette smoke exposure can downregulate the protein expressions of PGC-1α and COX4 during extensor digitorum longus (EDL) of rats in a significant intensity-dependent manner. Cigarette smoke exposure induced the downregulation of the gene expression of Sdhb and TFAM and reduced the SDH activity during soleus muscle of rats in a significant intensity- dependent manner. Electron microscopy presents cigarette smoke exposure induced vacuolar degeneration during EDL. Conclusion: Cigarette smoke exposure can induce the mitochondrial dysfunction in the skeletal muscles of rats, which is independent of the fiber type compositon of skeletal muscles. |
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