| 崔超扬,刘孟雪,余 意,龚吉红,阳小飞.L型多聚赖氨酸和D型多聚赖氨酸对神经细胞分化的影响[J].现代生物医学进展英文版,2019,19(14):2609-2613. |
| L型多聚赖氨酸和D型多聚赖氨酸对神经细胞分化的影响 |
| Influence of L-PL and D-PL on Nerve Cell Differentiation |
| Received:November 28, 2018 Revised:December 23, 2018 |
| DOI：10.13241/j.cnki.pmb.2019.14.002 |
| 中文关键词: L型多聚赖氨酸 D型多聚赖氨酸 神经细胞 分化率 |
| 英文关键词: Poly-L-lysine Poly-D-lysine Nerve cell Differentiation rate |
| 基金项目:国家自然科学基金项目(31670850)；中南民族大学科学基金引进人才科研启动基金自科项目(ZZ13002) |
| Author Name | Affiliation | E-mail | | CUI Chao-yang | Key Laboratory of Cognitive Science, Laboratory of Membrane Ion Channels and Medicine, College of Biomedical Engineering, College of Life Science, South-Central University for Nationalities, Wuhan, Hubei, 430074, China | 2451801053@qq.com | | LIU Meng-xue | Key Laboratory of Cognitive Science, Laboratory of Membrane Ion Channels and Medicine, College of Biomedical Engineering, College of Life Science, South-Central University for Nationalities, Wuhan, Hubei, 430074, China | | | YU Yi | Key Laboratory of Cognitive Science, Laboratory of Membrane Ion Channels and Medicine, College of Biomedical Engineering, College of Life Science, South-Central University for Nationalities, Wuhan, Hubei, 430074, China | | | GONG Ji-hong | Key Laboratory of Cognitive Science, Laboratory of Membrane Ion Channels and Medicine, College of Biomedical Engineering, College of Life Science, South-Central University for Nationalities, Wuhan, Hubei, 430074, China | | | YANG Xiao-fei | Key Laboratory of Cognitive Science, Laboratory of Membrane Ion Channels and Medicine, College of Biomedical Engineering, College of Life Science, South-Central University for Nationalities, Wuhan, Hubei, 430074, China | |
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| 中文摘要: |
| 摘要 目的：比较L型多聚赖氨酸(L-PL)和D型多聚赖氨酸(D-PL)浸泡的玻片培养小鼠大脑皮层神经元细胞对其分化的影响。方法：取昆明白小鼠的大脑皮层细胞，分别用L-PL和D-PL进行培养，统计和比较细胞的分化比率、突起数量以及突起生长长度。结果：L-PL和D-PL培养的神经细胞第1、2天的分化率比较差异具有统计学意义(P<0.05)，而突起数和突起长度比较差异无统计学意义(P>0.05)。当L-PL、D-PL的浓度分别为20 μL/mL、5 μL/mL时，其对1、2天细胞的分化产生显著影响，并且D型多聚赖氨酸培养的细胞分化率高于L型。500、100、20 μg/mL的L-PL中，100 μg/ml L-PL培养细胞的分化率最高，而125、25、5 μg/mL的D型多聚赖氨酸培养细胞分化率比较差异虽然无统计学意义，但25 μg/ml D-PL培养神经细胞的分化率总体趋势高于其他浓度。D-PL所形成的粒径大小与L-PL不同，且D-PL的总体趋势稍大于L-PL。结论：L型多聚赖氨酸和D型多聚赖氨酸会在神经细胞生长早期对分化率产生影响，但不影响突起数和突起长度。 |
| 英文摘要: |
| ABSTRACT Objective: To compare the effect of L-PL and D-PL soaked glass on the differentiation of nerve cells. Methods: The cerebral cortex cells of KM mice were cultured with D-PL and L-PL, respectively, the cell differentiation rate, branch number and branch length were counted. Results: The results showed that the differences of the differentiation rate but not the number and length of protuberances of nerve cells was significantly significant at day 1 and 2 between L-PL and D-PL(P<0.05). When the concentrations of L-PL and D-PL were 20 μg/mL and 5μg/mL respectively, the difference in differentiation rates of cells cultured on D-PL or L-PL at day 1 and 2 couldn't be eliminated , and the differentiation rate of cell cultured on D-PL was higher than that of L-PL. Among those 500, 100 and 20 μg/mL of L-PL, 100μg/ml L-PL had the highest differentiation rate. Similarly, among 125, 25 and 5 μg/mL of D-PL, although no statistical significance was found, 25 μg/mL D-PL showed a trend to have higher differentiation rate of nerve cells than other concentrations. Interestingly, bigger particle size was found in D-PL group than L-PL, it might contribute to the difference in the differentiation rates at early stage. Conclusion: L-PL and D-PL can affect the differentiation rate at the early stage of nerve cell growth, but do not affect the number of projections and the length of projections. |
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