| 闫小敏,王大秀,王 楠,李旭辉,杨小明,孟宪红,胡凤丽,韩继武.FGF-21抑制HSC-T6细胞活化的抗肝纤维化作用及其机制的研究[J].现代生物医学进展英文版,2019,19(11):2076-2080. |
| FGF-21抑制HSC-T6细胞活化的抗肝纤维化作用及其机制的研究 |
| Effect of Fibroblast Growth Factor 21 on Hepatic Stellate Cell-T6 and Its Mechanism |
| Received:March 06, 2019 Revised:March 30, 2019 |
| DOI：10.13241/j.cnki.pmb.2019.11.015 |
| 中文关键词: 成纤维细胞生长因子21 肝纤维化 Notch2 炎症反应 |
| 英文关键词: Fibroblast growth factor 21 Hepatic fibrosis Notch2 Inflammation |
| 基金项目:黑龙江省教育厅科技研究面上项目（12521357） |
| Author Name | Affiliation | E-mail | | YAN Xiao-min | Department of Gastroenterology, The fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150001, China | 1214571162@qq.com | | WANG Da-xiu | Department of Gastroenterology, The fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150001, China | | | WANG Nan | College of Life Sciences, Northeast Agricultural University, Harbin, Heilongjiang, 150030, China | | | LI Xu-hui | Heilongjiang province the Red Coss Hospital(Heilongjiang province Forestry General Hospital, Harbin, Heilongjiang, 150040, China | | | YANG Xiao-ming | Department of Gastroenterology, The fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150001, China | | | MENG Xian-hong | Department of Gastroenterology, The fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150001, China | | | HU Feng-li | Department of Gastroenterology, The fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150001, China | | | HAN Ji-wu | Department of Gastroenterology, The fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150001, China | |
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| 中文摘要: |
| 摘要 目的：探索成纤维细胞生长因子21（FGF-21）对肝星形细胞T6（HSC-T6）活化的作用及其作用机制。方法：1640+10 %胎牛血清的培养基培养HSC-T6细胞，实验分为5组：正常对照组（Control）、模型组（Model 20 mM乙醇处理细胞12 h）、低剂量FGF-21组（LFGF-21，0.5 μmol/L）、中剂量FGF-21组（MFGF-21，1.0 μmol/L）和高剂量FGF-21组（HFGF-21，2.0 μmol/L）。Real-time PCR检测α-平滑肌肌动蛋白（α-SMA）、胶原蛋白Ⅰ（CollagenⅠ）、基质金属蛋白酶-2（MMP2）、基质金属蛋白酶-9（MMP9）和Notch2的mRNA水平，Western blot检测CollagenⅠ、α-SMA、MMP2、MMP9和Notch2的蛋白水平，ELISA检测IL-1β、TNF-α的蛋白水平。结果：模型组α-SMA、CollagenⅠ、MMP2、MMP9、IL-1β、TNF-α、Notch2的水平高于对照组（P＜0.05），HFGF-21组α-SMA、CollagenⅠ、MMP2、MMP9、IL-1β、TNF-α、Notch2的水平均低于模型组（P＜0.05）。结论：FGF-21可抑制Notch2的表达，抑制炎症反应，从而抑制HSC的活化，发挥抗肝纤维化作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of fibroblast growth factor 21 (FGF-21) on hepatic stellate cell-T6(HSC-T6) and the mechanism of its action. Methods: Culturing HSC-T6 with 1640 medium supplemented with 10% fetal bovine serum and 1%antibiotics (penicillin-streptomycin). HSC-T6 were randomly divided into five groups: control group, model group (20 mM, ethanol), low dose FGF-21(LFGF-21, 0.5 μmol/L), middle dose FGF-21(MFGF-21, 1.0 μmol/L) and high dose FGF-21(HFGF-21, 2.0 μmol/L). The mRNA contents of α-SMA, CollagenⅠ, MMP2, MMP9 and Notch2 were determined by Real-time PCR. The protein levels of α-SMA, Colla- genⅠ, MMP2, MMP9 and Notch2 were determined by Western blot. The protein levels of IL-1β and TNF-α were measured by ELISA kit. Results: The levels of α-SMA, CollagenⅠ, MMP2, MMP9, IL-1β, TNF-α and Notch2 in the model group were significantly higher than those in the control group (P＜0.05), and those in the treatment group decreased significantly compared with the model group (P＜0.05). Conclusion: FGF-21 can inhibit the expression of Notch2 and inflammation, and then inhibit HSC activation to attenuate hepatic fibrogenesis. |
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