| 吴进荣,黄 凯,邹海峰,张崇友,马一鸣,张浩强,王丹丹,房绍红.Irgm 1参与动脉粥样硬化斑块的形成[J].现代生物医学进展英文版,2019,19(11):2046-2050. |
| Irgm 1参与动脉粥样硬化斑块的形成 |
| Irgm 1 is Involved in the Formation of Atherosclerotic Plaque |
| Received:January 23, 2019 Revised:February 18, 2019 |
| DOI：10.13241/j.cnki.pmb.2019.11.009 |
| 中文关键词: Irgm 1 ApoE-/-Irgm 1+/+小鼠 动脉粥样硬化 动脉血管斑块 |
| 英文关键词: Irgm1 ApoE-/- Irgm 1+/+ mice Atherosclerosis Arterial plaque |
| 基金项目:哈尔滨市科学技术局项目(2015RAQYJ105)；国家自然科学基金项目(81870353)；黑龙江省自然科学基金项目(QC2014C018)；黑龙江省教育厅项目(UNPYSCT-2015026) |
| Author Name | Affiliation | E-mail | | WU Jin-rong | Department of Anesthesiology, first affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150020, China | wujinrong1976@hotmail.com | | HUANG Kai | Department of Neurobiology, Heilongjiang Provincial Key Laboratory of Neurobiology, Harbin Medical University, Harbin, Heilongjiang, 150086, China | | | ZOU Hai-feng | Department of Neurobiology, Heilongjiang Provincial Key Laboratory of Neurobiology, Harbin Medical University, Harbin, Heilongjiang, 150086, China | | | ZHANG Chong-you | Department of Neurobiology, Heilongjiang Provincial Key Laboratory of Neurobiology, Harbin Medical University, Harbin, Heilongjiang, 150086, China | | | MA Yi-ming | Department of Neurobiology, Heilongjiang Provincial Key Laboratory of Neurobiology, Harbin Medical University, Harbin, Heilongjiang, 150086, China | | | ZHANG Hao-qiang | Department of Neurobiology, Heilongjiang Provincial Key Laboratory of Neurobiology, Harbin Medical University, Harbin, Heilongjiang, 150086, China | | | WANG Dan-dan | Department of Neurobiology, Heilongjiang Provincial Key Laboratory of Neurobiology, Harbin Medical University, Harbin, Heilongjiang, 150086, China | | | FANG Shao-hong | Department of Cardiology, second affiliated Hospital of Harbin Medical University, The Key Laboratory of Myocardial Ischemia, Harbin Medical University, Ministry of Education, Harbin, Heilongjiang, 150086, China | |
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| 中文摘要: |
| 摘要 目的：探讨免疫相关GTP酶1(Irgm 1)对小鼠血管动脉粥样硬化(AS)斑块形成的影响。方法：高脂饲料喂养野生型(WT)、ApoE-/-Irgm 1+/+和ApoE-/-Irgm1+/-小鼠3个月，建立AS模型；取小鼠主动脉弓，免疫荧光染色方法观察WT和ApoE-/-Irgm 1+/+小鼠血管AS斑块中Irgm 1的表达情况及部位；Western blot方法检测WT和ApoE-/-Irgm 1+/+小鼠血管AS斑块中Irgm 1蛋白表达情况；Q-PCR方法检测WT和ApoE-/-Irgm 1+/+小鼠血管AS斑块中Irgm 1 mRNA表达情况；油红O染色观察ApoE-/-Irgm1+/+和ApoE-/-Irgm1+/-小鼠血管AS斑块形成情况；结果：与WT组相比，ApoE-/-Irgm 1+/+组小鼠主动脉弓AS斑块中Irgm 1+细胞明显增多，Irgm 1+细胞主要位于血管AS斑块的表面；与WT组相比，ApoE-/-Irgm 1+/+组小鼠血管AS斑块中Irgm 1蛋白表达显著增多(P<0.001)，Irgm 1 mRNA表达显著增多(P<0.01)；与ApoE-/-Irgm1+/-组相比，ApoE-/-Irgm1+/+组小鼠主动脉弓AS斑块面积显著增大(P<0.01)；结论：Irgm 1能够促进血管AS斑块的形成。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of Immunity-related GTPase family M protein (Irgm1) on atherosclerotic (AS) plaque formation. Methods: Wild type (WT), ApoE-/-Irgm1+/+ and ApoE-/-Irgm1+/- mice were fed with high fat for 3 months to establish AS model. The expression of Irgm1 in WT and ApoE-/-Irgm1+/+ AS plaques was detected by immunofluorescence, Western blot was used to detect the expression of Irgm 1 protein in AS plaques in WT and ApoE-/-Irgm1+/+ mice, and the expression of Irgm 1 mRNA in AS plaques of these two groups was detected by Q-PCR. Finally, the formation of AS plaques in ApoE-/-Irgm1+/+ and ApoE-/-Irgm1+/- mice was observed by Oil red O staining. Results: Compared with WT group, the expression of Irgm 1 in AS plaque in ApoE-/-Irgm1+/+ mice were significantly increased (P<0.001), and the increased Irgm 1+ cells mainly concentrated on the surface of AS plaque; the Irgm1 mRNA as well as its protein were also found significantly increased (P<0.01) in ApoE-/-Irgm1+/+ mice, compared with WT group. The AS plaque area in ApoE-/-Irgm1+/- group was significantly larger than that in ApoE-/-Irgm1+/+ group (P<0.01). Conclusion: Irgm 1 can promote the for- mation of vascular AS plaque. |
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