| 苏保雄,梁 璐,方 瑜,葛繁梅,汪梅花,冯延丽.髓细胞性白血病患儿PTEN蛋白表达及其与免疫表型的关系[J].现代生物医学进展英文版,2019,19(10):1953-1957. |
| 髓细胞性白血病患儿PTEN蛋白表达及其与免疫表型的关系 |
| Expression of PTEN Protein and Its Relationship with Immunophenotype in Children with Myeloid Leukemia |
| Received:September 24, 2018 Revised:October 18, 2018 |
| DOI：10.13241/j.cnki.pmb.2019.10.032 |
| 中文关键词: 髓细胞性白血病 PTEN蛋白 免疫表型 相关性 |
| 英文关键词: Myeloid leukemia PTEN protein Immunophenotype Correlation |
| 基金项目:陕西省中医管理局中医药科研项目(JCMS036) |
| Author Name | Affiliation | E-mail | | SU Bao-xiong | Department of hematology, affiliated hospital of Yan 'an university in Shaanxi province, Yan'an, Shaanxi, 716000, China | Subaoxiong42378@163.com | | LIANG Lu | Department of anesthesiology, affiliated hospital of Yan 'an university in Shaanxi province, Yan'an Shaanxi, 716000, China | | | FANG Yu | Diagnostics teaching and research section, Shaanxi University Of Chinese Medicine, Xianyang, Shaanxi, 712046, China | | | GE Fan-mei | Department of hematology, affiliated hospital of Yan 'an university in Shaanxi province, Yan'an, Shaanxi, 716000, China | | | WANG Mei-hua | Department of hematology, affiliated hospital of Yan 'an university in Shaanxi province, Yan'an, Shaanxi, 716000, China | | | FENG Yan-li | Department of hematology, affiliated hospital of Yan 'an university in Shaanxi province, Yan'an, Shaanxi, 716000, China | |
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| 中文摘要: |
| 摘要 目的：探讨髓细胞性白血病(AML)患儿PTEN蛋白表达及其与免疫表型的关系。方法：选择AML患儿143例，根据免疫表型的不同分为两组：免疫分型为LY+AML型59例，LY-AML84例。所有患儿都给予免疫表型分析，检测PTEN蛋白表达情况并进行相关性分析。结果：LY+AML患儿CD34+阳性、CD117+阳性比例显著高于LY-AML患儿（P <0.05），染色体核型异常比例显著低于LY-AML患儿（P <0.05）。LY+AML患儿的PTEN蛋白表达量为（65.33±2.34）%，阳性表达率为94.9%；而LY-AML分别为（20.11±4.11）%和13.1%，与LY+AML患儿对比差异都有统计学意义(P <0.05)。在AML患儿中，Spearman相关分析显示PTEN蛋白表达水平与免疫分型呈现显著相关性(r =0.653，P =0.000)。多因素logistic回归方法显示PTEN蛋白表达、CD34+阳性、CD117+阳性、染色体核型异常为影响AML患儿免疫表型的主要独立危险因素(OR=1.098、1.045、1.092、0.294，P <0.05)。结论：AML患儿骨髓单个核细胞的PTEN蛋白表达上调使得LY+AML型发生风险显著增加，可作为AML患儿病情判断与预后预测的参考指标之一。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the expression of PTEN protein and its relationship with immunophenotype in children with acute myeloid leukemia (AML). Methods: 143 children with AML were selected. According to the different of immunophenotype, they were divided into two groups: 59 cases of LY+AML type and 84 cases of LY-AML. All the children were given immunophenotypic analysis, and the expression of PTEN protein were detected and given the correlation analysis. Results: The proportion of CD34+ positive and CD117+ positive in children with LY+AML were significantly higher than that in children with LY-AML (P <0.05), and the abnormal proportion of karyotype were significantly lower than that in children with LY-AML (P <0.05). The expression of PTEN protein in children with LY+AML were (65.33±2.34)%, and the positive expression rates were 94.9%. The LY-AML were (20.11±4.11)% and 13.1%, respectively, compared he difference were statistically significant in children with LY+AML(P <0.05). In children with AML, Spearman correlation analysis showed a significant correlation between PTEN protein expression and immunophenotyping (r =0.653, P=0.000). Multivariate logistic regression showed that PTEN protein expression, CD34+ positive, CD117+ positive, and karyotypic abnormalities were the main independent risk factors affected the immunophenotype of children with AML(OR =1.098, 1.045, 1.092, 0.294, P<0.05). Conclusion: The upregulation of PTEN protein expression in the bone marrow mononuclear cells can significantly increase the risk of LY+AML. It can be used as the reference index of the prognosis of children with AML. |
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