| 胡 朗,李 巍,唐代诗,亓秉超,邱继欢,常 盼,付 锋,李 妍.烟酰胺核糖抑制db/db小鼠糖尿病心肌病的作用及机制研究[J].现代生物医学进展英文版,2019,19(9):1644-1648. |
| 烟酰胺核糖抑制db/db小鼠糖尿病心肌病的作用及机制研究 |
| Study on Effect and Mechanism of Nicotinamide Ribose Inhibiting Diabetic Cardiomyopathy in db/db Mice |
| Received:October 21, 2018 Revised:November 17, 2018 |
| DOI：10.13241/j.cnki.pmb.2019.09.009 |
| 中文关键词: 烟酰胺核糖 糖尿病心肌病 沉默调节蛋白1 凋亡 氧化应激 |
| 英文关键词: Nicotinamide riboside Diabetic cardiomyopathy SIRT1 Apoptosis Oxidative stress |
| 基金项目:国家自然科学基金项目（81570252；81770369）；陕西省科技厅一般项目（2018SF-129） |
| Author Name | Affiliation | E-mail | | HU Lang | Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | medhlang@qq.com | | LI Wei | Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | | | TANG Dai-shi | Department of Endocrinology, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning, 116000, China | | | QI Bing-chao | Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | | | QIU Ji-huan | Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | | | CHANG Pan | Department of Physiology, School of Basic Medicine, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | | | FU Feng | Department of Physiology, School of Basic Medicine, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | | | LI Yan | Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | |
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| 中文摘要: |
| 摘要 目的：探讨烟酰胺核糖（NR）对2型糖尿病小鼠心肌病的治疗作用及其机制。方法：2型糖尿病模型db/db鼠和及其严格对照小鼠db/+小鼠，将小鼠分为Con (db/+)组，DM (db/db)组，DM+NR组。采用超声测小鼠心脏功能，western-blot及免疫组化测SIRT1表达含量，DHE染色、MDA含量和MnSOD活性检测反映氧化应激水平。结果：与对照组相比，db/db小鼠心脏功能显著下降(LVEF：42.3±7.2vs 73.7±10.2, P<0.01；LVFS：22.1±4.2vs 42.7±6.9, P<0.01)，SIRT1表达量显著下调（P<0.01）。NR喂养提高SIRT1表达量（P<0.01），并有效改善db/db小鼠心脏功能(LVEF：53.1±8.1vs 42.3±7.2, P<0.01；LVFS：33.4±6.9vs 22.1±4.2, P<0.01)。同时，NR喂养显著降低了db/db小鼠心肌组织的凋亡水平和氧化应激水平（P<0.05）。结论：NR有效改善了db/db小鼠的心功能障碍，降低了db/db小鼠的心肌凋亡水平和氧化应激水平，这些作用的发挥可能与NR增加SIRT1的表达量有关。 |
| 英文摘要: |
| ABSTRACT Objective: To clarify the therapeutic effect of nicotinamide ribose (NR) on type 2 diabetic myocardial injury in mice and its potential underlying mechanism. Methods: Type 2 diabetes model mice db/db mice and wild C57 mice were purchased at 8 weeks old. The mice were divided into Con(db/+) group, DM (db/db) group and DM+NR (db/db+NR) group. Echocardiology was used to mea- sure the heart function of mice. The expression of SIRT1 was detected by western-blot and immunohistochemistry. ROS level, MDA content and MnSOD activity was detected to reflect oxidative stress level. Results: Compared with the control group, the cardiac function of db/db mice decreased significantly (LVEF: 42.3±7.2vs 73.7±10.2, P<0.01; LVFS: 22.1±4.2vs 42.7±6.9, P<0.01), and the expres- sion of SIRT1 was significantly down-regulated (P<0.01). NR feeding increased SIRT1 expression (P<0.01) and effectively improved cardiac function in diabetic db/db mice (LVEF: 53.1±8.1vs 42.3±7.2, P<0.01; LVFS: 33.4±6.9vs 22.1±4.2, P<0.01). At the same time, NR feeding effectively reduced the level of apoptosis and oxidative stress in myocardial tissue of diabetic db/db mice (P<0.01). Conclusion: NR effectively improved cardiac dysfunction, reduced myocardial apoptosis and oxidative stress levels in diabetic db/db mice, and these effects may be exerted by the increased expression level of SIRT1 by NR. |
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